Biocontrol and Action Mechanism of Bacillus amyloliquefaciens and Bacillus subtilis in Soybean Phytophthora Blight

With the improper application of fungicides, Phytophthora sojae begins to develop resistance to fungicides, and biological control is one of the potential ways to control it. We screened two strains of Bacillus; Bacillus amyloliquefaciens JDF3 and Bacillus subtilis RSS-1, which had an efficient inhibitory effect on P. sojae. They could inhibit mycelial growth, the germination of the cysts, and the swimming of the motile zoospores. To elucidate the response of P. sojae under the stress of B. amyloliquefaciens and B. subtilis, and the molecular mechanism of biological control, comparative transcriptome analysis was applied. Transcriptome analysis revealed that the expression gene of P. sojae showed significant changes, and a total of 1616 differentially expressed genes (DEGs) were detected. They participated in two major types of regulation, namely “specificity” regulation and “common” regulation. They might inhibit the growth of P. sojae mainly by inhibiting the activity of ribosome. A pot experiment indicated that B. amyloliquefaciens and B. subtilis enhanced the resistance of soybean to P. sojae, and their control effects of them were 70.7% and 65.5%, respectively. In addition, B. amyloliquefaciens fermentation broth could induce an active oxygen burst, NO production, callose deposition, and lignification. B. subtilis could also stimulate the systemic to develop the resistance of soybean by lignification, and phytoalexin.

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Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

Frontiers in Microbiology ◽

10.3389/fmicb.2021.746799 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yanan Duan ◽

Ran Chen ◽

Rong Zhang ◽

Weitao Jiang ◽

Xuesen Chen ◽

...

Keyword(s):

Bacillus Amyloliquefaciens ◽

Fermentation Broth ◽

Biological Control Agent ◽

Lateral Roots ◽

Dna Gyrase ◽

Inhibitory Effect ◽

Dilution Method ◽

Fertilizer Treatment ◽

Strong Inhibitory Effect ◽

Subunit B

Apple replant disease (ARD) is a common problem in major apple planting areas, and biological factors play a leading role in its etiology. Here, we isolated the bacterial strain QSB-6 from the rhizosphere soil of healthy apple trees in a replanted orchard using the serial dilution method. Strain QSB-6 was provisionally identified as Bacillus amyloliquefaciens based on its morphology, physiological and biochemical characteristics, carbon source utilization, and chemical sensitivity. Maximum likelihood analysis based on four gene sequences [16S ribosomal RNA gene (16S rDNA), DNA gyrase subunit A (gyrA), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB)] from QSB-6 and other strains indicated that it had 100% homology with B. amyloliquefaciens, thereby confirming its identification. Flat standoff tests showed that strain QSB-6 had a strong inhibitory effect on Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Fusarium oxysporum, Alternaria alternata, Aspergillus flavus, Phoma sp., Valsa mali, Rhizoctonia solani, Penicillium brasilianum, and Albifimbria verrucaria, and it had broad-spectrum antibacterial characteristics. Extracellular metabolites from strain QSB-6 showed a strong inhibitory effect on Fusarium hyphal growth and spore germination, causing irregular swelling, atrophy, rupture, and cytoplasmic leakage of fungal hyphae. Analysis of its metabolites showed that 1,2-benzenedicarboxylic acid and benzeneacetic acid, 3- hydroxy-, methyl ester had good inhibitory effects on Fusarium, and increased the length of primary roots and the number of lateral roots of Arabidopsis thaliana plantlet. Pot experiments demonstrated that a QSB-6 bacterial fertilizer treatment (T2) significantly improved the growth of Malus hupehensis Rehd. seedlings. It increased root length, surface area, tips, and forks, respiration rate, protective enzyme activities, and the number of soil bacteria while reducing the number of soil fungi. Fermentation broth from strain QSB-6 effectively prevented root damage from Fusarium. terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (qPCR) assays showed that the T2 treatment significantly reduced the abundance of Fusarium in the soil and altered the soil fungal community structure. In summary, B. amyloliquefaciens QSB-6 has a good inhibitory effect on Fusarium in the soil and can significantly promote plant root growth. It has great potential as a biological control agent against ARD.

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Comparative Transcriptome Analysis of <italic>Setosphaeria turcica</italic> Revealed its Responses Mechanisms to the Biological Control Agent <italic>Bacillus amyloliquefaciens</italic>

Scientia Sinica Vitae ◽

10.1360/n052016-00166 ◽

2016 ◽

Vol 46 (5) ◽

pp. 627-636 ◽

Cited By ~ 1

Author(s):

null TIAN XueLiang, ZHANG Kai, WANG GuoLiang & LIU WenDe ◽

null 田雪亮, 张恺, 王国梁, 刘文德

Keyword(s):

Biological Control ◽

Transcriptome Analysis ◽

Bacillus Amyloliquefaciens ◽

Biological Control Agent ◽

Control Agent ◽

Comparative Transcriptome ◽

Setosphaeria Turcica ◽

Comparative Transcriptome Analysis

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Isolation and Identification of Bacillus amyloliquefaciens IBFCBF-1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper

Journal of Phytopathology ◽

10.1111/jph.12522 ◽

2016 ◽

Vol 164 (11-12) ◽

pp. 1012-1021 ◽

Cited By ~ 8

Author(s):

Mengjun Zhang ◽

Jilie Li ◽

Airong Shen ◽

Shiyong Tan ◽

Zhun Yan ◽

...

Keyword(s):

Biological Control ◽

Bacillus Amyloliquefaciens ◽

Growth Promotion ◽

Phytophthora Blight ◽

Isolation And Identification

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Draft Genomic Sequences of Bacillus amyloliquefaciens CBMAI 1301 and Bacillus subtilis CBMAI 1302, Strains with Potential for the Biocontrol of Phytopathogens

Microbiology Resource Announcements ◽

10.1128/mra.00321-21 ◽

2021 ◽

Vol 10 (33) ◽

Author(s):

Isabella Takahashi Kitano ◽

Iron Amoreli de Figueiredo Ribeiro ◽

Vitor Lima Coelho ◽

Carlos Alberto Xavier Gonçalves ◽

Giulia Naranjo Aranha ◽

...

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Bacillus Amyloliquefaciens ◽

Genomic Sequences ◽

Soybean Seeds ◽

Content Type ◽

Potential Applications

Here, we report the draft genomic sequences of Bacillus amyloliquefaciens strain CBMAI 1301, isolated from soybean seeds, and Bacillus subtilis strain CBMAI 1302, isolated from soil. These strains have potential applications for the biological control of phytopathogens, and the sequencing of these two genomes could greatly benefit soybean cultivation.

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