scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

scholarly journals Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

2016 ◽  
Vol 106 (11) ◽  
pp. 1344-1351 ◽  
Author(s):  
K. Esvelt Klos ◽  
T. Gordon ◽  
P. Bregitzer ◽  
P. Hayes ◽  
X. M. Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Resistance Qtl ◽  
Barley Stripe Rust

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a ‘Lenetah’ × ‘Grannelose Zweizeilige’ (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a ‘95SR316A’ × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

scholarly journals Effective Resistance to Wheat Stripe Rust in a Region with High Disease Pressure

Plant Disease ◽  
2014 ◽  
Vol 98 (7) ◽  
pp. 891-897 ◽  
Author(s):  
B. Bai ◽  
J. Y. Du ◽  
Q. L. Lu ◽  
C. Y. He ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Hot Spot ◽  
Infection Type ◽  
Durable Resistance ◽  
Gansu Province ◽  
Stripe Rust Resistance ◽  
Adult Plant

Stripe rust is a major fungal disease of wheat. It frequently becomes epidemic in southeastern Gansu province, a stripe rust hot spot in China. Evaluations of wheat germplasm response are crucial for developing cultivars to control the disease. In total, 57 wheat cultivars and lines from Europe and other countries, comprising 36 cultivars with documented stripe rust resistance genes and 21 with unknown genes, were tested annually with multiple races of Puccinia striiformis f. sp. tritici in the field at Tianshui in Gansu province from 1993 to 2013. Seven wheat lines were highly resistant, with infection type (IT) 0 during the entire period; 16 were moderately resistant (IT 0;-2); and 26 were moderately susceptible (IT 0;-4), with low maximum disease severity compared with the susceptible control Huixianhong. ‘Strampelli’ and ‘Libellula’, with three and five quantitative trait loci, respectively, for stripe rust resistance have displayed durable resistance in this region for four decades. Ten cultivars, including ‘Lantian 15’, ‘Lantian 26’, and ‘Lantian 31’, with stripe rust resistance derived from European lines, were developed in our breeding program and have made a significant impact on controlling stripe rust in southeastern Gansu. Breeding resistant cultivars with multiple adult-plant resistance genes seems to be a promising strategy in wheat breeding for managing stripe rust in this region and other hot spots.

scholarly journals Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars From the Huang-Huai-Hai Region of China

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1763-1770
Author(s):  
Liang Huang ◽  
Xing Zhi Xiao ◽  
Bo Liu ◽  
Li Gao ◽  
Guo Shu Gong ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Molecular Testing ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Yr Genes

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula

2012 ◽  
Vol 63 (6) ◽  
pp. 539 ◽  
Author(s):  
M. A. Asad ◽  
B. Bai ◽  
C. X. Lan ◽  
J. Yan ◽  
X. C. Xia ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Powdery Mildew ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Adult Plant

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a fungal disease that causes significant yield losses in many wheat-growing regions of the world. Previously, five quantitative trait loci (QTLs) for adult-plant resistance (APR) to stripe rust resistance were identified in Italian wheat cultivar Libellula. The objectives of this study were to map QTLs for APR to powdery mildew in 244 F2 : 3 lines of Libellula/Huixianhong, to analyse the stability of detected QTLs across environments, and to assess the association of these QTLs with stripe rust resistance. Powdery mildew response was evaluated for 2 years in Beijing and for 1 year in Anyang. The correlation between averaged maximum disease severity (MDS) and averaged area under disease progress curve (AUDPC) over 2 years in Beijing was 0.98, and heritabilities of MDS and AUDPC were 0.65 and 0.81, respectively, based on the mean values averaged across environments. SSR markers were used to screen the parents and mapping population. Five QTLs were identified by inclusive composite interval mapping, designated as QPm.caas-2DS, QPm.caas-4BL.1, QPm.caas-6BL.1, QPm.caas-6BL.2, and QPm.caas-7DS. Three QTLs (QPm.caas-2DS and QPm.caas-6BL.1, and QPm.caas-6BL.2) seem to be new resistance loci for powdery mildew. QTLs QPm.caas-2DS and QPm.caas-4BL.1 were identified at the same position as previously mapped QTLs for stripe rust resistance in Libellula. The QTL QPm.caas-7DS, derived from Libellula, coincided with the slow rusting and slow mildewing locus Lr34/Yr18/Pm38. These results and the identified markers could be useful for wheat breeders aiming for durable resistance to both powdery mildew and stripe rust.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Identification of Candidate Genes and Genomic Regions Associated with Adult Plant Resistance to Stripe Rust in Spring Wheat

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2585
Author(s):  
Amira M. I. Mourad ◽  
Mohamed A. Abou-Zeid ◽  
Shamseldeen Eltaher ◽  
P. Stephen Baenziger ◽  
Andreas Börner
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Yield ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
P Value ◽  
Dart Markers ◽  
Single Marker Analysis ◽  
Gene Models

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two growing seasons 2018/2019 and 2019/2020. A high genetic variation was found among the tested genotypes. Single marker analysis was conducted using a subset of 71 genotypes and 424 diversity array technology (DArT) markers, well distributed across the genome. Out of the tested markers, 13 stable markers were identified that were significantly associated with resistance in both years (p-value ≤ 0.05). By using the sequence of the DArT markers, the chromosomal position of the significant DArT markers was detected, and nearby gene models were identified. Two markers on chromosomes 5A and 5B were found to be located within gene models functionally annotated with disease resistance in plants. These two markers could be used in marker-assisted selection for stripe rust resistance under Egyptian conditions. Two German genotypes were carrying the targeted allele of all the significant DArT markers associated with stripe rust resistance and could be used to improve resistance under Egyptian conditions.

Genetics of stripe rust resistance in 'Karamu' wheat (Triticum aestivum L.)

2005 ◽  
Vol 56 (6) ◽  
pp. 619 ◽  
Author(s):  
M. Imtiaz ◽  
M. G. Cromey ◽  
J. G. Hampton ◽  
F. C. Ogbonnaya
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Triticum Aestivum L ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Dh Lines ◽  
Disease Pressure

The New Zealand wheat (Triticum aestivum L.) cv. Karamu (same parentage as the US cv. Anza) was originally believed to carry gene Yr18, which provides adult plant resistance to stripe rust (Puccinia striiformis f. sp. tritici), in addition to the seedling resistance gene YrA. Following the detection of virulence to the stripe rust resistance gene YrA in 1995, much of the resistance of Karamu was eroded and the cultivar suffered from occasional severe stripe rust outbreaks. This meant that either one or more new races of Puccinia striiformis f. sp. tritici with virulence to Yr18 had developed, or that Yr18 conferred inadequate resistance under high disease pressure. Karamu was crossed with cv. Otane, which carries Yr18, and 140 double haploid (DH) lines obtained from the F1 progeny were evaluated for seedling and adult plant resistance under greenhouse and field conditions. Evaluation of F1 plants against stripe rust pathotype 106E139A+ revealed that the resistance was recessive and that none of the resistance genes present was effective at the seedling stage. Segregation in the DH lines at the adult plant stage indicated that the resistance measured through infection type in both the greenhouse and the field was based on 3 genes, 1 from Karamu and 2 from Otane. However, the resistance gene from Karamu did not contribute towards resistance measured through final disease severity, but acted additively with genes from Otane in providing slow-rusting resistance as expressed by lower values for area under the disease progress curve. It was concluded that Karamu does not have gene Yr18, but rather possesses a recessive minor gene, which explains its adult plant susceptibility under high disease pressure. However, this Karamu gene did interact with Otane resistance genes to provide increased resistance.

Genetics of stripe rust resistance in two barley (Hordeum vulgare L.) genotypes

2019 ◽  
Vol 79 (02) ◽  
Author(s):  
Vishnu Kumar ◽  
S. C. Bhardwaj ◽  
A. S. Kharub ◽  
G. P. Singh
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
Malting Quality ◽  
Stripe Rust Resistance ◽  
Yield Reduction ◽  
Adult Plant ◽  
Hordeum Vulgare L ◽  
F2 Population

Stripe rust inflicted by the pathogen Puccinia striiformis Westend. f. sp. hordei Eriks can cause marked grain yield reduction and deterioration of the malting quality in barley. Two barley genotypes, DWRB137 (DWR28/DWRUB64) and DWRB143 (DWRB73/DWR83) were identified as resistant (R) for three stripe rust races viz., 57 (0S0), M (1S0) and Q (5S0) at seedling and adult plant stages. These genotypes were crossed with two stripe rust susceptible (S) barley cultivars, RD2035 and Lakhan to study mode of inheritance of stripe rust resistance. All the F1 plants showed resistance against stripe rust race, 57 (0S0) indicating that the resistance (R) is dominant. The F2 generations derived from the crosses namely, Lakhan/DWRB137, Lakhan/DWRB143, RD2035/DWRB137 and RD2035/DWRB143 were tested with inoculum of race, 57 (0S0) under artificially inoculated conditions. The observed frequency of segregants in each F2 generation fit well in a theoretical ratio of 3(R):1(S) (χ 2(T) less than 0.01) indicating that the resistance against the tested race is controlled by a single dominant gene in the genotypes, DWRB137 and DWRB143. To validate the F2 hypothesis, the F3 progenies were also tested under above described conditions and followed discrete segregation of 1 (R): 2 (Segregating): 1(S) ratio in all the four crosses. Test of allelism was also conducted to establish the identity of resistance gene(s) present in the resistant genotypes. The F2 population derived from DWRB137/DWRB143 (R × R) cross fit to 15(R):1(S) ratio showing that the two genotypes had different resistance genes.

scholarly journals Seedling and Slow Rusting Resistance to Stripe Rust in Chinese Common Wheats

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1302-1312 ◽  
Author(s):  
Z. F. Li ◽  
X. C. Xia ◽  
X. C. Zhou ◽  
Y. C. Niu ◽  
Z. H. He ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Gene Pyramiding ◽  
Stripe Rust Resistance ◽  
Slow Rusting ◽  
Cropping Seasons ◽  
Wheat Lines ◽  
Rust Resistance Genes

Identification of seedling and slow stripe rust resistance genes is important for gene pyramiding, gene deployment, and developing slow-rusting wheat cultivars to control the disease. A total of 98 Chinese lines were inoculated with 26 pathotypes of Puccinia striiformis f. sp. tritici for postulation of stripe rust resistance genes effective at the seedling stage. A total of 135 wheat lines were planted at two locations to characterize their slow rusting responses to stripe rust in the 2003-2004 and 2004-2005 cropping seasons. Genes Yr2, Yr3a, Yr4a, Yr6, Yr7, Yr9, Yr26, Yr27, and YrSD, either singly or in combinations, were postulated in 72 lines, whereas known resistance genes were not identified in the other 26 accessions. The resistance genes Yr9 and Yr26 were found in 42 and 19 accessions, respectively. Yr3a and Yr4a were detected in two lines, and four lines may contain Yr6. Three lines were postulated to possess YrSD, one carried Yr27, and one may possess Yr7. Thirty-three lines showed slow stripe rusting resistance at two locations in both seasons.

Stripe rust resistance among western Canadian spring wheat and triticale varieties

2012 ◽  
Vol 92 (4) ◽  
pp. 713-722 ◽  
Author(s):  
H. Randhawa ◽  
B. J. Puchalski ◽  
M. Frick ◽  
A. Goyal ◽  
T. Despins ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Spring Wheat ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Western Canada ◽  
Resistant Varieties ◽  
Plant Resistance Gene ◽  
Moderately Resistant

Randhawa, H., Puchalski, B. J., Frick, M., Goyal, A., Despins, T., Graf, R. J., Laroche, A. and Gaudet, D. A. 2012. Stripe rust resistance among western Canadian spring wheat and triticale varieties. Can. J. Plant Sci. 92: 713–722. Stripe rust (Puccinia striiformis Westend.) is an important pathogen of wheat in western Canada and worldwide. One hundred and four spring wheat and triticale varieties and cultivars were evaluated for resistance to stripe rust in nurseries at Lethbridge and Creston, BC, during 2009 and 2010. In the Canada Prairie Spring Red (CPSR) wheat class, newer varieties were more resistant compared with many of the older varieties. Among the white Canada Prairie Spring White (CPSW) wheats, Vista was moderately resistant, whereas Snowhite475 and Snowhite476 were susceptible. Little useful resistance was observed within the Canada Western Hard White Spring (CWHWS) class. Sixty percent of the Canada Western Red Spring (CWRS) wheats, including the currently popular varieties Lillian, Harvest and Kane, were resistant. Susceptible CWRS varieties that are extensively seeded in western Canada include AC Barrie, Superb and McKenzie, but also include the recently registered CDC Kernen and Vesper. The varieties were tested for the presence of the stripe rust genes Yr10, Yr17, Yr18 and Yr36 using molecular markers. Much of the stripe rust resistance, particularly in the CWRS, Canada Western Extra Strong (CWES), and CPSR wheat classes was attributed to the presence of adult plant resistance gene Yr18. Yr17 and Yr36 were also detected among CWRS and CWES varieties. However, the absence of markers for known genes in several resistant varieties indicated that uncharacterized genes for stripe rust occur among hexaploid wheats. Durum wheat and triticale varieties were universally resistant with the absence of tested markers. Therefore, there appear to be numerous sources of stripe rust resistance, both characterized and uncharacterized, among western Canadian spring wheat and triticale varieties.

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