scholarly journals Methyl Jasmonate Induced Oxidative Stress and Accumulation of Secondary Metabolites in Plant Cell and Organ Cultures

2020 ◽  
Vol 21 (3) ◽  
pp. 716 ◽  
Author(s):  
Thanh-Tam Ho ◽  
Hosakatte Niranjana Murthy ◽  
So-Young Park
Keyword(s):  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Plant Cell ◽  
Large Scale ◽  
Cultured Cells ◽  
Food Additives ◽  
Plant Secondary Metabolites ◽  
Signal Molecules ◽  
Scale Production ◽  
Organ Cultures

Recently, plant secondary metabolites are considered as important sources of pharmaceuticals, food additives, flavours, cosmetics, and other industrial products. The accumulation of secondary metabolites in plant cell and organ cultures often occurs when cultures are subjected to varied kinds of stresses including elicitors or signal molecules. Application of exogenous jasmonic acid (JA) and methyl jasmonate (MJ) is responsible for the induction of reactive oxygen species (ROS) and subsequent defence mechanisms in cultured cells and organs. It is also responsible for the induction of signal transduction, the expression of many defence genes followed by the accumulation of secondary metabolites. In this review, the application of exogenous MJ elicitation strategies on the induction of defence mechanism and secondary metabolite accumulation in cell and organ cultures is introduced and discussed. The information presented here is useful for efficient large-scale production of plant secondary metabolites by the plant cell and organ cultures.

scholarly journals Sekonder Bitki Metabolitlerinin In Vitro Koşullarda Üretimi

2019 ◽  
Vol 7 (7) ◽  
pp. 971
Author(s):  
Tuncay Çalışkan ◽  
Rüştü Hatipoğlu ◽  
Saliha Kırıcı
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Plant Cell ◽  
Abiotic Factors ◽  
Food Additives ◽  
Plant Secondary Metabolites ◽  
Secondary Metabolite Production ◽  
Organ Cultures ◽  
Metabolite Production

Plant secondary metabolites are a group of organic compounds produced by plants to interact with biotic and abiotic factors and for the establishment of defence mechanism. Secondary metabolites are classified based on their biosynthetic origin and chemical structure. They have been used as pharmaceutical, agrochemical, flavours, fragrances, colours and food additives. Secondary metabolites are traditionally produced from the native grown or field grown plants. However, this conventional approach has some disadvantages such as low yield, instability of secondary metabolite contents of the plants due to geographical, seasonal and environmental variations, need for land and heavy labour to grow plants. Therefore, plant cell and organ cultures have emerged as an alternative to plant growing under field conditions for secondary metabolite production. In this literature review, present state of secondary metabolite production through plant cell and organ cultures, its problems as well as solutions of the problems were discussed.

scholarly journals Alkaloids Production and Cell Growth of Cinchona ledgeriana Moens: Effects of Fungal Filtrate and Methyl Jasmonate Elicitors

2021 ◽  
Vol 6 (1) ◽  
pp. 31-40
Author(s):  
Yustiny Andaliza Hasibuan ◽  
Diah Ratnadewi ◽  
Zainal Alim Mas’ud
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Woody Plant ◽  
Cultured Cells ◽  
Cinchona Alkaloids ◽  
Cell Biomass ◽  
Cinchona Ledgeriana ◽  
Culture Technology

Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination.  It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor than M-23 for this plant species. Filtrate of non-virulent fungi can elevate the biosynthesis of alkaloids. This reconfirms that cultured cells are capable to produce secondary metabolites and the productivity can be increased by using an appropriate elicitor.  

scholarly journals Large-scale production of pharmaceutical proteins in plant cell culture-the protalix experience

2015 ◽  
Vol 13 (8) ◽  
pp. 1199-1208 ◽  
Author(s):  
Yoram Tekoah ◽  
Avidor Shulman ◽  
Tali Kizhner ◽  
Ilya Ruderfer ◽  
Liat Fux ◽  
...  
Keyword(s):  
Cell Culture ◽  
Plant Cell ◽  
Plant Cell Culture ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production ◽  
Pharmaceutical Proteins

scholarly journals Remarkable Ability of Pandoraea pnomenusa B356 Biphenyl Dioxygenase To Metabolize Simple Flavonoids

2012 ◽  
Vol 78 (10) ◽  
pp. 3560-3570 ◽  
Author(s):  
Thi Thanh My Pham ◽  
Youbin Tu ◽  
Michel Sylvestre
Keyword(s):  
Secondary Metabolites ◽  
Plant Secondary Metabolites ◽  
Structural Features ◽  
Signal Molecules ◽  
Supporting Evidence ◽  
Catabolic Pathway ◽  
Ecological Functions ◽  
Content Type ◽  
Burkholderia Xenovorans ◽  
Double Substitution

ABSTRACTMany investigations have provided evidence that plant secondary metabolites, especially flavonoids, may serve as signal molecules to trigger the abilities of bacteria to degrade chlorobiphenyls in soil. However, the bases for this interaction are largely unknown. In this work, we found that BphAEB356, the biphenyl/chlorobiphenyl dioxygenase fromPandoraea pnomenusaB356, is significantly better fitted to metabolize flavone, isoflavone, and flavanone than BphAELB400fromBurkholderia xenovoransLB400. Unlike those of BphAELB400, the kinetic parameters of BphAEB356toward these flavonoids were in the same range as for biphenyl. In addition, remarkably, the biphenyl catabolic pathway of strain B356 was strongly induced by isoflavone, whereas none of the three flavonoids induced the catabolic pathway of strain LB400. Docking experiments that replaced biphenyl in the biphenyl-bound form of the enzymes with flavone, isoflavone, or flavanone showed that the superior ability of BphAEB356over BphAELB400is principally attributable to the replacement of Phe336 of BphAELB400by Ile334 and of Thr335 of BphAELB400by Gly333 of BphAEB356. However, biochemical and structural comparison of BphAEB356with BphAEp4, a mutant of BphAELB400which was obtained in a previous work by the double substitution Phe336Met Thr335Ala of BphAELB400, provided evidence that other residues or structural features of BphAEB356whose precise identification the docking experiment did not allow are also responsible for the superior catalytic abilities of BphAEB356. Together, these data provide supporting evidence that the biphenyl catabolic pathways have evolved divergently among proteobacteria, where some of them may serve ecological functions related to the metabolism of plant secondary metabolites in soil.

scholarly journals Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

2021 ◽  
Author(s):  
Christoph Wawrosch ◽  
Sergey B. Zotchev
Keyword(s):  
Tissue Culture ◽  
Metabolic Engineering ◽  
Secondary Metabolites ◽  
Chemical Synthesis ◽  
Plant Cell ◽  
Plant Secondary Metabolites ◽  
Heterologous Production ◽  
In Vitro Production ◽  
Vitro Production

AbstractMedicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cultivated raw material. The biotechnological manufacturing of such compounds offers a number of advantages like predictable, stable, and year-round sustainable production, scalability, and easier extraction and purification. Plant cell and tissue culture represents one possible alternative to the extraction of phytochemicals from plant material. Although a broad commercialization of such processes has not yet occurred, ongoing research indicates that plant in vitro systems such as cell suspension cultures, organ cultures, and transgenic hairy roots hold a promising potential as sources for bioactive compounds. Progress in the areas of biosynthetic pathway elucidation and genetic manipulation has expanded the possibilities to utilize plant metabolic engineering and heterologous production in microorganisms. This review aims to summarize recent advances in the in vitro production of high-value plant secondary metabolites of medicinal importance.Key points• Bioactive plant secondary metabolites are important for current and future use in medicine• In vitro production is a sustainable alternative to extraction from plants or costly chemical synthesis• Current research addresses plant cell and tissue culture, metabolic engineering, and heterologous production Graphical abstract

scholarly journals Serratia marcescens Quinoprotein Glucose Dehydrogenase Activity Mediates Medium Acidification and Inhibition of Prodigiosin Production by Glucose

2012 ◽  
Vol 78 (17) ◽  
pp. 6225-6235 ◽  
Author(s):  
James E. Fender ◽  
Cody M. Bender ◽  
Nicholas A. Stella ◽  
Roni M. Lahr ◽  
Eric J. Kalivoda ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Serratia Marcescens ◽  
Large Scale ◽  
Transcriptional Control ◽  
Glucose Dehydrogenase ◽  
Model Organism ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
Scale Production ◽  
Content Type

ABSTRACTSerratia marcescensis a model organism for the study of secondary metabolites. The biologically active pigment prodigiosin (2-methyl-3-pentyl-6-methoxyprodiginine), like many other secondary metabolites, is inhibited by growth in glucose-rich medium. Whereas previous studies indicated that this inhibitory effect was pH dependent and did not require cyclic AMP (cAMP), there is no information on the genes involved in mediating this phenomenon. Here we used transposon mutagenesis to identify genes involved in the inhibition of prodigiosin by glucose. Multiple genetic loci involved in quinoprotein glucose dehydrogenase (GDH) activity were found to be required for glucose inhibition of prodigiosin production, including pyrroloquinoline quinone and ubiquinone biosynthetic genes. Upon assessing whether the enzymatic products of GDH activity were involved in the inhibitory effect, we observed thatd-glucono-1,5-lactone andd-gluconic acid, but notd-gluconate, were able to inhibit prodigiosin production. These data support a model in which the oxidation ofd-glucose by quinoprotein GDH initiates a reduction in pH that inhibits prodigiosin production through transcriptional control of the prodigiosin biosynthetic operon, providing new insight into the genetic pathways that control prodigiosin production. Strains generated in this report may be useful in large-scale production of secondary metabolites.

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