scholarly journals Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

2020 ◽  
Vol 21 (13) ◽  
pp. 4705
Author(s):  
Jiang Xiong ◽  
Lian Liu ◽  
Xiaochuan Ma ◽  
Feifei Li ◽  
Chaolan Tang ◽  
...  
Keyword(s):  
Poncirus Trifoliata ◽  
Luciferase Assay ◽  
Allene Oxide ◽  
Biological Processes ◽  
Allene Oxide Synthase ◽  
Trifoliate Orange ◽  
Chloroplast Targeting ◽  
Novel Proteins ◽  
Oxide Synthase

Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P−532 (−532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P−532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.

scholarly journals Characterization of novel imidazole derivative, JM-8686, a potent inhibitor of allene oxide synthase

FEBS Letters ◽  
2006 ◽  
Vol 580 (24) ◽  
pp. 5791-5796 ◽  
Author(s):  
Keimei Oh ◽  
Tadao Asami ◽  
Kenji Matsui ◽  
Gregg A. Howe ◽  
Noboru Murofushi
Keyword(s):  
Potent Inhibitor ◽  
Imidazole Derivative ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Oxide Synthase

Characterization of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Artemisia annua

2011 ◽  
Vol 39 (3) ◽  
pp. 2267-2274 ◽  
Author(s):  
Xu Lu ◽  
Fangyuan Zhang ◽  
Weimin Jiang ◽  
Xiuyan Lin ◽  
Yunfei Chen ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Artemisia Annua ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Pathway Gene ◽  
Oxide Synthase

scholarly journals Wounding stimulates ALLENE OXIDE SYNTHASE gene and increases the level of jasmonic acid in Ipomoea nil cotyledons

2016 ◽  
Vol 85 (1) ◽  
Author(s):  
Emilia Wilmowicz ◽  
Agata Kućko ◽  
Kamil Frankowski ◽  
Barbara Zabrocka-Nowakowska ◽  
Katarzyna Panek ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Stress Responses ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Lipoxygenase Pathway ◽  
Ipomoea Nil ◽  
Evolutionarily Conserved ◽  
Strong Stimulation ◽  
Oxide Synthase

<em>Allene oxide synthase</em> (<em>AOS</em>) encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA) formation. In this study we report the molecular cloning and characterization of <em>InAOS</em> from <em>Ipomoea nil</em>. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 motif, which is evolutionarily conserved and characteristic for functional enzymatic proteins. We have shown that wounding led to a strong stimulation of the examined gene activity in cotyledons and an increase in JA level, which suggest that this compound may be a modulator of stress responses in <em>I. nil</em>.

Molecular Characterization of the Gene Encoding Rice Allene Oxide Synthase and Its Expression

2002 ◽  
Vol 66 (12) ◽  
pp. 2719-2722 ◽  
Author(s):  
Suk-Bong HA ◽  
Bong-Choon LEE ◽  
Da-Eun LEE ◽  
Yong In KUK ◽  
A-Young LEE ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Gene Encoding ◽  
Oxide Synthase

scholarly journals Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

2006 ◽  
Vol 281 (51) ◽  
pp. 38981-38988 ◽  
Author(s):  
Bénédicte Bakan ◽  
Mats Hamberg ◽  
Ludivine Perrocheau ◽  
Daniel Maume ◽  
Hélène Rogniaux ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Lipid Transfer ◽  
Plant Lipid ◽  
Transfer Protein ◽  
Oxide Synthase

scholarly journals Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

2018 ◽  
Vol 19 (8) ◽  
pp. 2440 ◽  
Author(s):  
Qiyuan Peng ◽  
Ying Zhou ◽  
Yinyin Liao ◽  
Lanting Zeng ◽  
Xinlan Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Jasmonic Acid ◽  
Camellia Sinensis ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Tea Leaves ◽  
Ethylene Formation ◽  
Horticultural Crops ◽  
Metabolite Profiles ◽  
Oxide Synthase

Jasmonic acid (JA) is reportedly involved in the interaction between insects and the vegetative parts of horticultural crops; less attention has, however, been paid to its involvement in the interaction between insects and the floral parts of horticultural crops. Previously, we investigated the allene oxide synthase 2 (AOS2) gene that was found to be the only JA synthesis gene upregulated in tea (Camellia sinensis) flowers exposed to insect (Thrips hawaiiensis (Morgan)) attacks. In our present study, transient expression analysis in Nicotiana benthamiana plants confirmed that CsAOS2 functioned in JA synthesis and was located in the chloroplast membrane. In contrast to tea leaves, the metabolite profiles of tea flowers were not significantly affected by 10 h JA (2.5 mM) treatment as determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, and gas chromatography-mass spectrometry. Moreover, JA treatment did not significantly influence ethylene formation in tea flowers. These results suggest that JA in tea flowers may have different functions from JA in tea leaves and other flowers.

scholarly journals Jasmonates: what ALLENE OXIDE SYNTHASE does for plants

2019 ◽  
Vol 70 (13) ◽  
pp. 3373-3378 ◽  
Author(s):  
Edward E Farmer ◽  
Alain Goossens
Keyword(s):  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Oxide Synthase
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