Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line

Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 revealed some differentiation capacity and the simultaneous presence of known MSC markers CD73, CD90, and CD105 even after continuous long-term culture for more than one year and 32 passages. The expression of CD13, CD29, CD44, and CD166 were identified as further surface proteins, all of which were also simultaneously detectable in various other types of primary MSC populations derived from the umbilical cord, bone marrow, and placenta suggesting MSC-like properties in the cell line. Proliferating steady state MSC544 exhibited immune-modulatory activity similar to a subpopulation of long-term growth-inhibited MSC544 after 189d of continuous culture in confluency. This confluent connective cell layer with fibroblast-like morphology can spontaneously contract and the generated space is subsequently occupied by new cells with regained proliferative capacity. Accordingly, the confluent and senescence-associated beta-galactosidase-positive MSC544 culture with about 95% G0/G1 growth-arrest resumed re-entry into the proliferative cell cycle within 3d after sub-confluent culture. The MSC544 cells remained viable during confluency and throughout this transition which was accompanied by marked changes in the release of proteins. Thus, expression of proliferation-associated genes was down-modulated in confluent MSC544 and re-expressed following sub-confluent conditions whilst telomerase (hTERT) transcripts remained detectable at similar levels in both, confluent growth-arrested and proliferating MSC544. Together with the capability of connective cell layer formation for potential therapeutic approaches, MSC544 provide a long term reproducible human cell source with constant properties.

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Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

BMC Cancer ◽

10.1186/1471-2407-8-291 ◽

2008 ◽

Vol 8 (1) ◽

Cited By ~ 4

Author(s):

Camila ML Machado ◽

Rafael Y Ikemori ◽

Tatiana Q Zorzeto ◽

Ana CMA Nogueira ◽

Suse DS Barbosa ◽

...

Keyword(s):

Cell Line ◽

Glioma Cell ◽

Glioma Cell Line

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LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA

Journal of Experimental Medicine ◽

10.1084/jem.140.2.494 ◽

1974 ◽

Vol 140 (2) ◽

pp. 494-507 ◽

Cited By ~ 31

Author(s):

M. E. Jobin ◽

J. L. Fahey ◽

Z. Price

Keyword(s):

Multiple Myeloma ◽

Cell Line ◽

Human Fibroblasts ◽

Myeloma Protein ◽

Culture Viability ◽

Feeder Layers ◽

Normal Human ◽

Stimulating Factor

Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons.

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Characterization of a new chromosomal marker for acute lymphoblastic leukemia from a long-term cell line

Cancer Genetics and Cytogenetics ◽

10.1016/0165-4608(82)90073-5 ◽

1982 ◽

Vol 7 (3) ◽

pp. 257-269 ◽

Cited By ~ 8

Author(s):

R.J. Green ◽

H.W. Findley ◽

A.T.L. Chen ◽

A.H. Ragab

Keyword(s):

Acute Lymphoblastic Leukemia ◽

Cell Line ◽

Lymphoblastic Leukemia ◽

Chromosomal Marker

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Characterization of a long-term rat mTAL cell line

AJP Renal Physiology ◽

10.1152/ajprenal.00426.2006 ◽

2007 ◽

Vol 293 (4) ◽

pp. F1413-F1422 ◽

Cited By ~ 19

Author(s):

Ben Eng ◽

Somshuvra Mukhopadhyay ◽

Carlos P. Vio ◽

Paulina L. Pedraza ◽

Shoujin Hao ◽

...

Keyword(s):

Atpase Activity ◽

Cell Line ◽

Specific Activity ◽

Primary Cultures ◽

Pcr Analysis ◽

Specific Marker ◽

Thick Ascending Limb ◽

Medullary Thick Ascending Limb

A medullary thick ascending limb (mTAL) cell line, termed raTAL, has been established from freshly isolated rat mTAL tubules and cultured continuously for up to 75 passages; it retains characteristics of mTAL cells even after retrieval from storage in liquid nitrogen for several months. The cells express Tamm-Horsfall glycoprotein (THP), a TAL-specific marker, grow to confluence, exhibit a polygonal morphology characteristic of epithelial cells, and form “domes.” Detection of THP, Na+-K+-2Cl− cotransporter (NKCC2), Na+-K+-ATPase, and renal outer medullary K+ channel (ROMK) was achieved using indirect immunofluorescence and confocal microscopy. Western blot analysis of NKCC2 expression using two different antibodies revealed a band of ∼160 kDa, and RT-PCR analysis demonstrated the presence of NKCC2 isoforms A and F, which was confirmed by DNA sequencing; transport of Cl− into raTAL cells was inhibited by furosemide. Ouabain- and bumetanide-sensitive oxygen consumption, an index of ion transport activity in the mTAL, was observed in raTAL cells, and the number of domes present was reduced significantly when cells were incubated in the presence of ouabain or bumetanide. The specific activity of Na+-K+-ATPase activity was determined in raTAL cells (0.67 ± 0.18 nmol Pi·μg protein−1·min−1), primary cultures of mTAL cells (0.39 ± 0.08 nmol Pi·μg protein−1·min−1), and freshly isolated mTAL tubules (1.10 ± 0.29 nmol Pi·μg protein−1·min−1), and ∼30–50% of total cellular ATPase activity was inhibited by ouabain, in accord with other mTAL preparations. This cell line will be used in studies that address biochemical, molecular, and physiological mechanisms in the mTAL.

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Characterization of a long-term T-helper-cell line which produces IL-2 and induces immunoglobulin secretion

Cellular Immunology ◽

10.1016/0008-8749(84)90275-2 ◽

1984 ◽

Vol 85 (1) ◽

pp. 34-44 ◽

Cited By ~ 2

Author(s):

Florence Tsui ◽

Brian J. Underdown ◽

Michel Klein ◽

Barry Zimmerman

Keyword(s):

Cell Line ◽

T Helper Cell ◽

Helper Cell ◽

T Helper ◽

Immunoglobulin Secretion

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Characterization of a canine long-term T cell line (DLC 01) established from a dog with Sézary syndrome and producing retroviral particles

Leukemia ◽

10.1038/sj.leu.2401480 ◽

1999 ◽

Vol 13 (8) ◽

pp. 1281-1290 ◽

Cited By ~ 15

Author(s):

I Ghernati ◽

C Auger ◽

L Chabanne ◽

A Corbin ◽

C Bonnefont ◽

...

Keyword(s):

T Cell ◽

Cell Line ◽

Sézary Syndrome ◽

Sezary Syndrome

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Characterization of Artificial Pneumothorax-Unrelated Pyothorax-Associated Lymphoma

Journal of Oncology ◽

10.1155/2021/3869438 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Guang-Liang Chen ◽

Zu-Guang Xia ◽

Jia Jin ◽

Bao-Hua Yu ◽

Junning Cao

Keyword(s):

Treatment Guideline ◽

B Cell Lymphoma ◽

Barr Virus ◽

Artificial Pneumothorax ◽

History Of ◽

Tuberculous Pleurisy ◽

Epstein Barr ◽

One Year

Pyothorax-associated lymphoma (PAL) is a rare disease developing from a long-term pleural cavity inflammation. Most reported PAL cases have a history of artificial pneumothorax. However, the clinical features of artificial pneumothorax-unrelated PAL remain largely unknown. Here, we reported two PAL cases diagnosed from our center in the past ten years. One case developed from asymptomatic pyothorax after pneumonectomy with a latency of 28 years, while the other case showed a relatively short latency of one year. Then we reviewed the literature of artificial pneumothorax-unrelated PAL by searching PubMed and Google Scholar from 2007. In total, nine artificial pneumothorax-unrelated PAL cases were found, predominantly in old male with median age of 76 years (ranging from 51 to 88). Most cases were diagnosed with diffuse large B-cell lymphoma (DLBCL) (n = 8, 88.9%) and had evidence of Epstein-Barr virus (EBV) infection (n = 6, 66.7%) or tuberculous pleurisy (n = 5, 55.6%). Notably, four cases (44.4%) had short intervals (no more than two years) between pleuritis and PAL. Regarding the overall survival, one-third cases survived more than 5 years after the diagnosis of PAL. In conclusion, the features of artificial pneumothorax-unrelated PAL are comparable with the classic type of PAL, except for some patients with short duration of pleuritis, and need to be identified. Treatment guideline of DLBCL is recommended for the management of PAL.

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