scholarly journals LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA

1974 ◽  
Vol 140 (2) ◽  
pp. 494-507 ◽  
Author(s):  
M. E. Jobin ◽  
J. L. Fahey ◽  
Z. Price
Keyword(s):  
Multiple Myeloma ◽  
Cell Line ◽  
Human Fibroblasts ◽  
Myeloma Protein ◽  
Culture Viability ◽  
Feeder Layers ◽  
Normal Human ◽  
Stimulating Factor

Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons.

scholarly journals Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.

1996 ◽  
Vol 183 (6) ◽  
pp. 2551-2558 ◽  
Author(s):  
A L Petzer ◽  
P W Zandstra ◽  
J M Piret ◽  
C J Eaves
Keyword(s):  
Ex Vivo ◽  
Granulocyte Colony Stimulating Factor ◽  
Flt3 Ligand ◽  
Serum Free ◽  
Feeder Layers ◽  
Normal Human ◽  
Growth Factor Beta ◽  
Necessary And Sufficient ◽  
Stimulating Factor

A high proportion of the CD34+CD38- cells in normal human marrow are defined as long-term culture-initiating cells (LTC-IC) because they can proliferate and differentiate when co-cultured with cytokine-producing stromal feeder layers. In contrast, very few CD34+CD38- cells will divide in cytokine-containing methylcellulose and thus are not classifiable as direct colony-forming cells (CFC), although most can proliferate in serum-free liquid cultures containing certain soluble cytokines. Analysis of the effects of 16 cytokines on CD34+CD38- cells in the latter type of culture showed that Flt3-ligand (FL), Steel factor (SF), and interleukin (IL)-3 were both necessary and sufficient to obtain an approximately 30-fold amplification of the input LTC-IC population within 10 d. As single factors, only FL and thrombopoietin (TPO) stimulated a net increase in LTC-IC within 10 d. Interestingly, a significantly increased proportion of the CFC produced from the TPO-amplified LTC-IC were erythroid. Increases in the number of directly detectable CFC of > 500-fold were also obtainable within 10 d in serum-free cultures of CD34+CD38- cells. However, this required the presence of IL-6 and/or granulocyte/colony-stimulating factor and/or nerve growth factor beta in addition to FL, SF, and IL-3. Also, for this response, the most potent single-acting factor tested was IL-3, not FL. Identification of cytokine combinations that differentially stimulate primitive human hematopoietic cell self-renewal and lineage determination should facilitate analysis of the intracellular pathways that regulate these decisions as well as the development of improved ex vivo expansion and gene transfer protocols.

scholarly journals Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

2021 ◽  
Vol 51 ◽  
pp. 102193
Author(s):  
Jose Inzunza ◽  
Jonathan Arias-Fuenzalida ◽  
Juan Segura-Aguilar ◽  
Ivan Nalvarte ◽  
Mukesh Varshney
Keyword(s):  
Cell Line ◽  
Human Fibroblasts ◽  
Ips Cell ◽  
Normal Human

scholarly journals Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

BMC Cancer ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Camila ML Machado ◽  
Rafael Y Ikemori ◽  
Tatiana Q Zorzeto ◽  
Ana CMA Nogueira ◽  
Suse DS Barbosa ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line

scholarly journals Localized and indolent myeloma

Blood ◽  
1980 ◽  
Vol 56 (3) ◽  
pp. 521-525 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Multiple Myeloma ◽  
Bone Lesions ◽  
Term Outcome ◽  
Myeloma Protein ◽  
Long Term Outcome ◽  
Long Term Follow Up ◽  
Tumor Load ◽  
Localized Disease

Abstract Criteria were defined for recognizing 29 patients with a localized plasmacytoma of bone and 20 patients with an indolent variety of multiple myeloma in order to justify long-term follow-up without chemotherapy. All patients with indolent myeloma were asymptomatic from their low tumor mass disease, had a hemoglobin greater than 10 g/dl, and showed no more than 3 lytic bone lesions. The presence of more than 200 mg/day of Bence Jones protein was usually followed by disease progression within 2 yr. Serial assessments of myeloma protein level provided a useful index of changing tumor load and the need for chemotherapy. In patients with localized disease, radiotherapy usually reduced myeloma proteins markedly with subsequent disease control for many years, even though small serum peaks persisted. Chemotherapy for multiple myeloma was not required for a median of 8 yr in patients presenting with localized disease and of 3 yr in those with indolent myeloma. The additional survival from the start of drug treatment was similar to that of comparable patients treated promptly for overt multiple myeloma. The delay of chemotherapy until evidence of tumor progression did not affect the long-term outcome of patients with localized or indolent myeloma.

scholarly journals Design, Synthesis, and Structural Characterization of Novel Diazaphenothiazines with 1,2,3-Triazole Substituents as Promising Antiproliferative Agents

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4388 ◽  
Author(s):  
Morak-Młodawska ◽  
Pluta ◽  
Latocha ◽  
Jeleń ◽  
Kuśmierz
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Human Fibroblasts ◽  
Human Tumor ◽  
Design Synthesis ◽  
Human Tumor Cell Lines ◽  
Ic50 Values ◽  
Normal Human ◽  
Low Cytotoxicity

A series of novel 1,2,3-triazole-diazphenothiazine hybrids was designed, synthesized, and evaluated for anticancer activity against four selected human tumor cell lines (SNB-19, Caco-2, A549, and MDA-MB231). The majority of the synthesized compounds exhibited significant potent activity against the investigated cell lines. Among them, compounds 1d and 4c showed excellent broad spectrum anticancer activity, with IC50 values ranging from 0.25 to 4.66 μM and 0.25 to 6.25 μM, respectively. The most promising compound 1d, possessing low cytotoxicity against normal human fibroblasts NHFF, was used for gene expression analysis using reverse transcription–quantitative real-time PCR (RT–qPCR). The expression of H3, TP53, CDKN1A, BCL-2, and BAX genes revealed that these compounds inhibited the proliferation in all cells (H3) and activated mitochondrial events of apoptosis (BAX/BCL-2).

scholarly journals Characterization of cytotoxic effect of asbestos on normal human fibroblasts.

1983 ◽  
Vol 51 ◽  
pp. 237-239 ◽  
Author(s):  
M J Chang ◽  
N P Singh ◽  
A Turturro ◽  
R W Hart
Keyword(s):  
Cytotoxic Effect ◽  
Human Fibroblasts ◽  
Normal Human

Characterization of a new IgGλ myeloma plasma cell line (EJM): a further tool in the investigation of the biology of multiple myeloma

1990 ◽  
Vol 75 (3) ◽  
pp. 378-384 ◽  
Author(s):  
M. S. Hamilton ◽  
J. Ball ◽  
E. Bromidge ◽  
J. Lowe ◽  
I. M. Franklin
Keyword(s):  
Multiple Myeloma ◽  
Cell Line ◽  
Plasma Cell

scholarly journals Cell cycle progression after cleavage failure

2004 ◽  
Vol 165 (5) ◽  
pp. 609-615 ◽  
Author(s):  
Yumi Uetake ◽  
Greenfield Sluder
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Mammalian Cells ◽  
Cell Cycle Progression ◽  
Human Fibroblasts ◽  
Human Cell Line ◽  
Cycle Progression ◽  
Binucleate Cells ◽  
Normal Human ◽  
Cleavage Failure

Failure of cells to cleave at the end of mitosis is dangerous to the organism because it immediately produces tetraploidy and centrosome amplification, which is thought to produce genetic imbalances. Using normal human and rat cells, we reexamined the basis for the attractive and increasingly accepted proposal that normal mammalian cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1, thereby preventing their propagation. Using 10 μM cytochalasin to block cleavage, we confirm that most binucleate cells arrest in G1. However, when we use lower concentrations of cytochalasin, we find that binucleate cells undergo DNA synthesis and later proceed through mitosis in >80% of the cases for the hTERT-RPE1 human cell line, primary human fibroblasts, and the REF52 cell line. These observations provide a functional demonstration that the tetraploidy checkpoint does not exist in normal mammalian somatic cells.

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