scholarly journals Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes

2020 ◽  
Vol 21 (18) ◽  
pp. 6704
Author(s):  
Alexander Kopp ◽  
Laura Schunck ◽  
Martin Gosau ◽  
Ralf Smeets ◽  
Simon Burg ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Tensile Testing ◽  
Biomedical Applications ◽  
Toxic Chemicals ◽  
Membrane Properties ◽  
Limited Extent ◽  
Dissolution Method ◽  
Process Output ◽  
Silkworm Bombyx Mori

In this study, we describe the manufacturing and characterization of silk fibroin membranes derived from the silkworm Bombyx mori. To date, the dissolution process used in this study has only been researched to a limited extent, although it entails various potential advantages, such as reduced expenses and the absence of toxic chemicals in comparison to other conventional techniques. Therefore, the aim of this study was to determine the influence of different fibroin concentrations on the process output and resulting membrane properties. Casted membranes were thus characterized with regard to their mechanical, structural and optical assets via tensile testing, SEM, light microscopy and spectrophotometry. Cytotoxicity was evaluated using BrdU, XTT, and LDH assays, followed by live–dead staining. The formic acid (FA) dissolution method was proven to be suitable for the manufacturing of transparent and mechanically stable membranes. The fibroin concentration affects both thickness and transparency of the membranes. The membranes did not exhibit any signs of cytotoxicity. When compared to other current scientific and technical benchmarks, the manufactured membranes displayed promising potential for various biomedical applications. Further research is nevertheless necessary to improve reproducible manufacturing, including a more uniform thickness, less impurity and physiological pH within the membranes.

Fabrication and characterization of silk fibroin–gelatin/chondroitin sulfate/hyaluronic acid scaffold for biomedical applications

2014 ◽  
Vol 126 ◽  
pp. 207-210 ◽  
Author(s):  
Nopporn Sawatjui ◽  
Teerasak Damrongrungruang ◽  
Wilairat Leeanansaksiri ◽  
Patcharee Jearanaikoon ◽  
Temduang Limpaiboon
Keyword(s):  
Hyaluronic Acid ◽  
Chondroitin Sulfate ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Fabrication And Characterization

Fabrication and characterization of flexible silk fibroin films reinforced with graphene oxide for biomedical applications

RSC Advances ◽  
2014 ◽  
Vol 4 (76) ◽  
pp. 40312-40320 ◽  
Author(s):  
Lu Wang ◽  
Chunxiang Lu ◽  
Baoping Zhang ◽  
Bin Zhao ◽  
Feng Wu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Fabrication And Characterization

scholarly journals Preparation and Characterization of Electrospun PCL/Silk Fibroin Scaffolds

2021 ◽  
Vol 35 (1) ◽  
pp. 31-42
Author(s):  
Emi Govorčin Bajsić ◽  
T. Holjevac Grgurić ◽  
Budimir Mijović ◽  
I. Vrgoč Zimić ◽  
T. Dolenec ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Degree Of Crystallinity ◽  
Surface Wetting ◽  
Wetting Ability ◽  
Electrospun Scaffolds ◽  
Model Cell ◽  
The Degree Of Crystallinity ◽  
Wide Usage

Natural polymer-based scaffolds are generally considered as favourable matrices for <br /> the adhesion and growth of cells in tissue repair. One of the most popular materials in <br /> this respect is silk fibroin, known for its wide usage in biomedical applications. This work focuses on the development of electrospun scaffolds based on poly(ε-caprolactone) <br /> (PCL) and silk fibroin (SF) evaluated regarding the SF effect on their morphology, surface wetting ability, thermal properties, and HaCaT model cell line biocompatibility. The <br /> study revealed that the lowest PCL/SF concentration resulted in highest bead-like morphology formation, relatively thick fibers with the presence of random beads in the case of PCL, while uniform and thinner fibers in the case of increasing PCL/SF content scaffolds. The addition of SF reduced the degree of crystallinity in the PCL due to the less <br /> organized crystal structure, and decreased its thermal stability. Both SEM and MTT analyses showed cell presence on all scaffolds three days after cell seeding. Although SF <br /> improved PCL hydrophilicity, as shown quantitatively by the MTT assay for improved <br /> cytocompatibility properties, more structured electrospun PCL/SF scaffold strategies are <br /> required.

Preparation and Characterization of Nano-β-Tricalcium Phosphate/Silk Fibroin Scaffold for Tissue Engineering Applications

2011 ◽  
Vol 343-344 ◽  
pp. 882-888
Author(s):  
Jun Ou ◽  
Yu Min Jiang ◽  
Zhan He Zhang
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Tricalcium Phosphate ◽  
Biomedical Applications ◽  
3D Scaffold ◽  
Ft Ir ◽  
Ir Analysis ◽  
Cultivation Experiment

Silk fibroin (SF) and β-tricalcium phosphate (β-TCP) had been used in biomedical applications for these years. The potential of silk and β-TCP for application in tissue engineering is currently being explored. The purpose of this study was to prepare and characterize a 3D scaffold consisting of nano-β-TCP/SF composite. XRD and FT-IR analysis showed that predominant crystalline phase of calcium phosphate was β-TCP; a chelate effect between SF and Ca2+ was happened at complexing period of SF and β-TCP. The compressive strength of nano-β-TCP/SF composite was 42 MPa ± 0.12 MPa. In vitro cell cultivation experiment showed that the composite was a good matrix for the growth of osteoblasts. Conclusion: the incorporation of SF into nano-β-TCP can enhance both mechanical strength and bioactivity of the scaffold, which suggests that the β-TCP/SF composite may be a potential biomaterial for tissue engineering.

scholarly journals How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1510
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Magnetic Particles ◽  
Chemical Properties ◽  
Ternary Mixtures ◽  
Cross Linking ◽  
Advantages And Disadvantages ◽  
Physico Chemical ◽  
Binary And Ternary Mixtures ◽  
To Receive

This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

scholarly journals Comparative Study of Silk Fibroin-Based Hydrogels and Their Potential as Material for 3-Dimensional (3D) Printing

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3887
Author(s):  
Watcharapong Pudkon ◽  
Chavee Laomeephol ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont ◽  
Juthamas Ratanavaraporn
Keyword(s):  
3D Printing ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Structure Stability ◽  
3 Dimensional ◽  
Critical Technology ◽  
Box Structure ◽  
High Degree

Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1–3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.

Sign in / Sign up

Export Citation Format

Share Document