scholarly journals PGS/HAp Microporous Composite Scaffold Obtained in the TIPS-TCL-SL Method: An Innovation for Bone Tissue Engineering

2021 ◽  
Vol 22 (16) ◽  
pp. 8587
Author(s):  
Paweł Piszko ◽  
Marcin Włodarczyk ◽  
Sonia Zielińska ◽  
Małgorzata Gazińska ◽  
Przemysław Płociński ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Composite Scaffold ◽  
In Vitro Models ◽  
Structure Characterization ◽  
Precipitation Method ◽  
Water Contact ◽  
Thermally Induced ◽  
Salt Leaching ◽  
Biological Potential

In this research, we synthesize and characterize poly(glycerol sebacate) pre-polymer (pPGS) (1H NMR, FTiR, GPC, and TGA). Nano-hydroxyapatite (HAp) is synthesized using the wet precipitation method. Next, the materials are used to prepare a PGS-based composite with a 25 wt.% addition of HAp. Microporous composites are formed by means of thermally induced phase separation (TIPS) followed by thermal cross-linking (TCL) and salt leaching (SL). The manufactured microporous materials (PGS and PGS/HAp) are then subjected to imaging by means of SEM and µCT for the porous structure characterization. DSC, TGA, and water contact angle measurements are used for further evaluation of the materials. To assess the cytocompatibility and biological potential of PGS-based composites, preosteoblasts and differentiated hFOB 1.19 osteoblasts are employed as in vitro models. Apart from the cytocompatibility, the scaffolds supported cell adhesion and were readily populated by the hFOB1.19 preosteoblasts. HAp-facilitated scaffolds displayed osteoconductive properties, supporting the terminal differentiation of osteoblasts as indicated by the production of alkaline phosphatase, osteocalcin and osteopontin. Notably, the PGS/HAp scaffolds induced the production of significant amounts of osteoclastogenic cytokines: IL-1β, IL-6 and TNF-α, which induced scaffold remodeling and promoted the reconstruction of bone tissue. Initial biocompatibility tests showed no signs of adverse effects of PGS-based scaffolds toward adult BALB/c mice.

scholarly journals Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Ran Zhang ◽  
Xuewen Li ◽  
Yao Liu ◽  
Xiaobo Gao ◽  
Tong Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Marrow Mesenchymal Stem Cells

Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by in vitro and in vivo experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics in vitro and in a critical sized defect in Sprague-Dawley rats in vivo. It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts in vitro and induced more bone formation in vivo. To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.

Fabrication, characterisation and in vitro biological activities of a sulfuretin-supplemented nanofibrous composite scaffold for tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (56) ◽  
pp. 44943-44952 ◽  
Author(s):  
YoungWon Koo ◽  
Hyeongjin Lee ◽  
Suji Kim ◽  
No-Joon Song ◽  
Jin-Mo Ku ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Biological Activities ◽  
Tissue Growth ◽  
Biological Component

A biocomposite consisting of PCL/BMP-2 and sulfuretin/alginate was proposed. Evaluation of in vitro cellular activities demonstrated that the sulfuretin can act as an outstanding biological component for enhancing bone tissue growth.

scholarly journals Inhibition of SYK and cSrc kinases can protect bone and cartilage in preclinical models of osteoarthritis and rheumatoid arthritis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
F. N. Novikov ◽  
M. V. Panova ◽  
I. Y. Titov ◽  
V. S. Stroylov ◽  
O. V. Stroganov ◽  
...  
Keyword(s):  
Bone Tissue ◽  
In Vitro Models ◽  
Multikinase Inhibitor ◽  
Monosodium Iodoacetate ◽  
Disease Modifying ◽  
Cartilage Erosion ◽  
Disease Modifying Treatment ◽  
And Cartilage

AbstractThe pathophysiology of osteoarthritis (OA) includes the destruction of subchondral bone tissue and inflammation of the synovium. Thus, an effective disease-modifying treatment should act on both of these pathogenetic components. It is known that cSrc kinase is involved in bone and cartilage remodeling, and SYK kinase is associated with the inflammatory component. Thus the aim of this study was to characterize the mechanism of action and efficacy of a small molecule multikinase inhibitor MT-SYK-03 targeting SYK and cSrc kinases among others in different in vitro and in vivo arthritis models. The selectivity of MT-SYK-03 kinase inhibition was assayed on a panel of 341 kinases. The compound was evaluated in a set of in vitro models of OA and in vivo OA and RA models: surgically-induced arthritis (SIA), monosodium iodoacetate-induced arthritis (MIA), collagen-induced arthritis (CIA), adjuvant-induced arthritis (AIA). MT-SYK-03 inhibited cSrc and SYK with IC50 of 14.2 and 23 nM respectively. Only five kinases were inhibited > 90% at 500 nM of MT-SYK-03. In in vitro OA models MT-SYK-03 reduced hypertrophic changes of chondrocytes, bone resorption, and inhibited SYK-mediated inflammatory signaling. MT-SYK-03 showed preferential distribution to joint and bone tissue (in rats) and revealed disease-modifying activity in vivo by halving the depth of cartilage erosion in rat SIA model, and increasing the pain threshold in rat MIA model. Chondroprotective and antiresorptive effects were shown in a monotherapy regime and in combination with methotrexate (MTX) in murine and rat CIA models; an immune-mediated inflammation in rat AIA model was decreased. The obtained preclinical data support inhibition of cSrc and SYK as a viable strategy for disease-modifying treatment of OA. A Phase 2 clinical study of MT-SYK-03 is to be started.

scholarly journals Preparation and Evaluation of Biocompatible Composite for Bone Tissue Engineering

2017 ◽  
Vol 4 (3) ◽  
pp. 7-14
Author(s):  
Masud Rana Md. ◽  
Naznin Akhtar ◽  
Zahid Hasan Md. ◽  
Asaduzzaman S M
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Application ◽  
Bone Tissue Regeneration ◽  
Precipitation Method ◽  
Tissue Engineering Scaffolds ◽  
Fixed Amount ◽  
Co Precipitation

Bone tissue engineering with cells and synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissue compared with the transplantation of bone. Hydroxyapatite (HA) and its composite with biopolymer are extensively developed and applied in bone tissue regeneration. The main aim of this study was to fabricate and characterize of HA apatite based biocompatible scaffold for bone tissue engineering. Scaffolds with different ratio of polymers (chitosan & alginate), and fixed amount of synthetic HA were prepared using in situ co precipitation method and mineral to polymer ratio was 1:1 to 1: 2 . A cross linker agent, 2-Hydroxylmethacrylate (HEMA) was added at different percentage (0.5-2%) into the selected composition and irradiated at 5- 25 kGy to optimize the proper mixing of components at the presence of HEMA. Fabricated scaffolds were analyzed to determine porosity, density, biodegradability, morphology and structural properties. Porosity and density of the prepared scaffold were 75 to 92% and 0.21 to 0.42 g/cm3 respectively. However, the swelling ratio of the fabricated scaffolds was ranged from 133 to 197%. Nonetheless, there had a reasonable in-vitro degradation of prepared scaffolds. Flourier transform infrared spectroscopy (FTIR) analysis showed intermolecular interaction between components in the scaffold. Pore size of scaffold was measured by scanning electron microscope and the value was 162-510 μm. It could be proposed that this scaffold fulfills all the main requirements to be considered as a bone substitute for biomedical application in near future.

scholarly journals Nanometric Hydroxyapatite Particles as Active Ingredient for Bioinks: A Review

Macromol ◽  
2022 ◽  
Vol 2 (1) ◽  
pp. 20-29
Author(s):  
Edilberto Ojeda ◽  
África García-Barrientos ◽  
Nagore Martínez de Cestafe ◽  
José María Alonso ◽  
Raúl Pérez-González ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Active Ingredient ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Temporal Organization ◽  
Advanced Materials ◽  
Special Focus ◽  
Spatio Temporal ◽  
Biomimetic Hydroxyapatite

Additive manufacturing (AM), frequently cited as three-dimensional (3D) printing, is a relatively new manufacturing technique for biofabrication, also called 3D manufacture with biomaterials and cells. Recent advances in this field will facilitate further improvement of personalized healthcare solutions. In this regard, tailoring several healthcare products such as implants, prosthetics, and in vitro models, would have been extraordinarily arduous beyond these technologies. Three-dimensional-printed structures with a multiscale porosity are very interesting manufacturing processes in order to boost the capability of composite scaffolds to generate bone tissue. The use of biomimetic hydroxyapatite as the main active ingredient for bioinks is a helpful approach to obtain these advanced materials. Thus, 3D-printed biomimetic composite designs may produce supplementary biological and physical benefits. Three-dimensional bioprinting may turn to be a bright solution for regeneration of bone tissue as it enables a proper spatio-temporal organization of cells in scaffolds. Different types of bioprinting technologies and essential parameters which rule the applicability of bioinks are discussed in this review. Special focus is made on hydroxyapatite as an active ingredient for bioinks design. The goal of such bioinks is to reduce the constraints of commonly applied treatments by enhancing osteoinduction and osteoconduction, which seems to be exceptionally promising for bone regeneration.

scholarly journals The Osteogenesis of Bone Marrow Stem Cells on mPEG-PCL-mPEG/Hydroxyapatite Composite Scaffold via Solid Freeform Fabrication

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Han-Tsung Liao ◽  
Yo-Yu Chen ◽  
Yu-Ting Lai ◽  
Ming-Fa Hsieh ◽  
Cho-Pei Jiang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Solid Freeform Fabrication ◽  
Bone Marrow Stem Cells ◽  
High Porosity ◽  
Tissue Scaffold

The study described a novel bone tissue scaffold fabricated by computer-aided, air pressure-aided deposition system to control the macro- and microstructure precisely. The porcine bone marrow stem cells (PBMSCs) seeded on either mPEG-PCL-mPEG (PCL) or mPEG-PCL-mPEG/hydroxyapatite (PCL/HA) composite scaffold were cultured under osteogenic medium to test the ability of osteogenesisin vitro. The experimental outcomes indicated that both scaffolds possessed adequate pore size, porosity, and hydrophilicity for the attachment and proliferation of PBMSCs and the PBMSCs expressed upregulated genes of osteogensis and angiogenesis in similar manner on both scaffolds. The major differences between these two types of the scaffolds were the addition of HA leading to higher hardness of PCL/HA scaffold, cell proliferation, and VEGF gene expression in PCL/HA scaffold. However, thein vivobone forming efficacy between PBMSCs seeded PCL and PCL/HA scaffold was different from thein vitroresults. The outcome indicated that the PCL/HA scaffold which had bone-mimetic environment due to the addition of HA resulted in better bone regeneration and mechanical strength than those of PCL scaffold. Therefore, providing a bone-mimetic scaffold is another crucial factor for bone tissue engineering in addition to the biocompatibility, 3D architecture with high porosity, and interpored connection.

Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110557-110565 ◽  
Author(s):  
Yinxian Yu ◽  
Sha Hua ◽  
Mengkai Yang ◽  
Zeze Fu ◽  
Songsong Teng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Tissue Engineering Scaffold ◽  
Fabrication And Characterization

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.

Production of Bioactive Nano-Hydroxyapatite/Polysaccharide Composites for Bone Tissue Engineering

2008 ◽  
Vol 587-588 ◽  
pp. 22-26
Author(s):  
Ana L. Daniel-da-Silva ◽  
A.M. Gil ◽  
Rui N. Correia
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Mechanical Performance ◽  
Calcium Phosphates ◽  
Thermally Induced ◽  
Co Precipitation

Porous κ-carrageenan based composites with potential application in bone tissue engineering have been prepared by in situ co-precipitation of nanoparticles of calcium phosphates, followed by thermally induced gelification and freeze-drying. The scaffolds showed macroporous structure with interconnected porosity. The variation of the biopolymer concentration affected the microstructure and compressive mechanical performance of the composites. The in vitro bioactivity was assessed by soaking the composites in simulated body fluid (SBF) and the formation of an apatite layer on their surface was found.

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