scholarly journals Possible Treatment of Myocardial Infarct Based on Tissue Engineering Using a Cellularized Solid Collagen Scaffold Functionalized with Arg-Glyc-Asp (RGD) Peptide

2021 ◽  
Vol 22 (22) ◽  
pp. 12563
Author(s):  
Olivier Schussler ◽  
Pierre E. Falcoz ◽  
Juan C. Chachques ◽  
Marco Alifano ◽  
Yves Lecarpentier
Keyword(s):  
Tissue Engineering ◽  
Ventricular Remodeling ◽  
Myocardial Infarct ◽  
Three Dimensional ◽  
Collagen Scaffold ◽  
Rgd Peptide ◽  
Polymer Backbone ◽  
Cell Engraftment ◽  
Contractile Tissue

Currently, the clinical impact of cell therapy after a myocardial infarction (MI) is limited by low cell engraftment due to low cell retention, cell death in inflammatory and poor angiogenic infarcted areas, secondary migration. Cells interact with their microenvironment through integrin mechanoreceptors that control their survival/apoptosis/differentiation/migration and proliferation. The association of cells with a three-dimensional material may be a way to improve interactions with their integrins, and thus outcomes, especially if preparations are epicardially applied. In this review, we will focus on the rationale for using collagen as a polymer backbone for tissue engineering of a contractile tissue. Contractilities are reported for natural but not synthetic polymers and for naturals only for: collagen/gelatin/decellularized-tissue/fibrin/Matrigel™ and for different material states: hydrogels/gels/solids. To achieve a thick/long-term contractile tissue and for cell transfer, solid porous compliant scaffolds are superior to hydrogels or gels. Classical methods to produce solid scaffolds: electrospinning/freeze-drying/3D-printing/solvent-casting and methods to reinforce and/or maintain scaffold properties by reticulations are reported. We also highlight the possibility of improving integrin interaction between cells and their associated collagen by its functionalizing with the RGD-peptide. Using a contractile patch that can be applied epicardially may be a way of improving ventricular remodeling and limiting secondary cell migration.

scholarly journals Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149799 ◽  
Author(s):  
Elsa C. Chan ◽  
Shyh-Ming Kuo ◽  
Anne M. Kong ◽  
Wayne A. Morrison ◽  
Gregory J. Dusting ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Collagen Scaffold ◽  
Engineering Applications

scholarly journals RGD Collagen for Engineering a Contractile Tissue and Cell Therapy after Myocardial Infarct

2021 ◽  
Author(s):  
Olivier schussler ◽  
pierre-emmanuel falcoz ◽  
Juan-Carlos Chachques ◽  
Marco Alifano ◽  
Yves lecarpentier
Keyword(s):  
Cell Therapy ◽  
Heart Development ◽  
Myocardial Infarct ◽  
Clinical Impact ◽  
Cardiac Cells ◽  
3D Scaffold ◽  
Secondary Migration ◽  
Cell Engraftment ◽  
Tumor Extract ◽  
Contractile Tissue

Currently, the clinical impact of cell therapy after a myocardial infarction (MI) is limited by low cell engraftment due to significant cell death, including apoptosis, in an infarcted, inflammatory, poor angiogenic environment, low cell retention and secondary migration. Cells interact with their environment through integrin mechanoreceptors that control their survival/apoptosis/differentiation/migration/proliferation. Optimizing these interactions may be a way of improving outcomes. The association of free cells with a 3D-scaffold may be a way to target their integrins. Collagen is the most abundant structural component of the extracellular matrix (ECM) and the best contractility levels are achieved with cellular preparations containing collagen, fibrin, or Matrigel (i.e. tumor extract). In the interactions between cells and ECM, 3 main proteins are recognised: collagen, laminin and RGD (Arg-Gly-Asp) peptide. The RGD plays a key role in heart development, after MI, and on cardiac cells. Cardiomyocytes secrete their own laminin on collagen. The collagen has a non-functional cryptic RGD and is thus suboptimal for interactions with associated cells. The use of a collagen functionalized with RGD may help to improve collagen biofunctionality. It may help in the delivery of paracrine cells, whether or not they are contractile, and in assisting tissue engineering a safe contractile tissue.

An administration on artificial skin for burning patient skin recovery through tissue engineering

2020 ◽  
Vol 04 ◽  
Author(s):  
Sourav Kumar Das
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Silk Fibroin ◽  
Human Body ◽  
Research Work ◽  
Collagen Scaffold ◽  
Artificial Skin ◽  
Skin Substitutes ◽  
Degumming Process

Background: Artificial skin has been tried to implement on subject which is damaged by burn effect.In this research i tried with Silk fibroin as a block biopolymer and which is very high biodegradability and more flexible for cell culture as being a part of human body.With this artificial skin of SF/Chitosan will help to produce more pores of tissue engineering which will create a good implantation system.Skin is the longest and most important organ of human body.Artificial skin is a collagen scaffold that causes skin regeneration in animals such as humans. The innovative biomaterial silk consists of this collagen.I also tried to introduce collagen consisting of silk nanofibers for human body linked to burning patients impacted areas via tissue engineering administration.Body comprises of three layers.They are sometimes called the hypodermis, epidermis, dermis and the fat layer. Purpose: An exploration to implement human compatible artificial skin for burned body. Materials and methods: At first turned silk fibroin through degumming process as a block biopolymer and then check oxygen permeability with silk fibroin and chitosan combination.Check mechanical properties and this total experiment done through tissue engineering and cell culture. Tissue-built skin substitutes may offer a viable helpful choice for the treatment of patients with skin harms. Results: Implementable long term sustainability and high biodegradability artificial skin through cell culture. Conclusion: In this research, artificial skin has been developed with chitosan/SF layer based with complete block biopolymer and i got a significant result which will be compatible for human original skin with high oxygen and water permeability with cell culture and it is effective and result oriented for burning patient skin recovery and accidental case in the replacement of skin. Discussion: Artificial skin is a collagen scaffold that induces regeneration of skin in mammals such as humans.The novel biomaterial silk consists of this collagen.In this research work,we tried to implement collagen consisting silk nanofibers for artificial skin for human body related to burning patients affected areas through tissue engineering administration.

Reversible physical crosslinking strategy with optimal temperature for 3D bioprinting of human chondrocyte-laden gelatin methacryloyl bioink

2018 ◽  
Vol 33 (5) ◽  
pp. 609-618 ◽  
Author(s):  
Yawei Gu ◽  
Lei Zhang ◽  
Xiaoyu Du ◽  
Ziwen Fan ◽  
Long Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Optimal Temperature ◽  
Chondrocyte Viability ◽  
Human Chondrocyte ◽  
Tunable Mechanical Properties ◽  
Dimensional Cell ◽  
Physical Crosslinking ◽  
Better Than

Gelatin methacryloyl is a promising material in tissue engineering and has been widely studied in three-dimensional bioprinting. Although gelatin methacryloyl possesses excellent biocompatibility and tunable mechanical properties, its poor printability/processability has hindered its further applications. In this study, we report a reversible physical crosslinking strategy for precise deposition of human chondrocyte-laden gelatin methacryloyl bioink at low concentration without any sacrificial material by using extrusive three-dimensional bioprinting. The precise printing temperature was determined by the rheological properties of gelatin methacryloyl with temperature. Ten percent (w/v) gelatin methacryloyl was chosen as the printing formula due to highest biocompatibility in three-dimensional cell cultures in gelatin methacryloyl hydrogel disks. Primary human chondrocyte-laden 10% (w/v) gelatin methacryloyl was successfully printed without any construct deformation or collapse and was permanently crosslinked by ultraviolet light. The printed gelatin methacryloyl hydrogel constructs remained stable in long-term culture. Chondrocyte viability and proliferation that were printed under this optimal temperature were better than that of chondrocytes printed under lower temperatures and were similar to that of chondrocytes in the non-printed gelatin methacryloyl hydrogels. The results indicate that with this strategy, 10% (w/v) gelatin methacryloyl bioink presented excellent printability and printing resolution with high cell viability, which appears to be suitable for printing primary human chondrocytes in cartilage biofabrication and can be extensively applied in tissue engineering of other organs or in other biomedical fields.

Practical electron tomography

1995 ◽  
Vol 53 ◽  
pp. 742-743
Author(s):  
C.L. Woodcock
Keyword(s):  
Electron Tomography ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3D Shape ◽  
Computational Resources ◽  
Shape Of Particles

Despite the potential of the technique, electron tomography has yet to be widely used by biologists. This is in part related to the rather daunting list of equipment and expertise that are required. Thanks to continuing advances in theory and instrumentation, tomography is now more feasible for the non-specialist. One barrier that has essentially disappeared is the expense of computational resources. In view of this progress, it is time to give more attention to practical issues that need to be considered when embarking on a tomographic project. The following recommendations and comments are derived from experience gained during two long-term collaborative projects.Tomographic reconstruction results in a three dimensional description of an individual EM specimen, most commonly a section, and is therefore applicable to problems in which ultrastructural details within the thickness of the specimen are obscured in single micrographs. Information that can be recovered using tomography includes the 3D shape of particles, and the arrangement and dispostion of overlapping fibrous and membranous structures.

Custom Presurgical Planning for Midfacial Reconstruction

2020 ◽  
Vol 36 (06) ◽  
pp. 696-702
Author(s):  
Nolan B. Seim ◽  
Enver Ozer ◽  
Sasha Valentin ◽  
Amit Agrawal ◽  
Mead VanPutten ◽  
...  
Keyword(s):  
Multidisciplinary Approach ◽  
Functional Performance ◽  
Three Dimensional ◽  
Free Tissue Transfer ◽  
Long Term Effects ◽  
Presurgical Planning ◽  
Dental Rehabilitation ◽  
Midface Reconstruction ◽  
Reconstructive Algorithm

AbstractResection and reconstruction of midface involve complex ablative and reconstructive tools in head and oncology and maxillofacial prosthodontics. This region is extraordinarily important for long-term aesthetic and functional performance. From a reconstructive standpoint, this region has always been known to present challenges to a reconstructive surgeon due to the complex three-dimensional anatomy, the variable defects created, combination of the medical and dental functionalities, and the distance from reliable donor vessels for free tissue transfer. Another challenge one faces is the unique features of each individual resection defect as well as individual patient factors making each preoperative planning session and reconstruction unique. Understanding the long-term effects on speech, swallowing, and vision, one should routinely utilize a multidisciplinary approach to resection and reconstruction, including head and neck reconstructive surgeons, prosthodontists, speech language pathologists, oculoplastic surgeons, dentists, and/or craniofacial teams as indicated and with each practice pattern. With this in mind, we present our planning and reconstructive algorithm in midface reconstruction, including a dedicated focus on dental rehabilitation via custom presurgical planning.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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