scholarly journals Development of an Autochthonous Microbial Consortium for Enhanced Bioremediation of PAH-Contaminated Soil

2021 ◽  
Vol 22 (24) ◽  
pp. 13469
Author(s):  
Marta Roszak ◽  
Joanna Jabłońska ◽  
Xymena Stachurska ◽  
Kamila Dubrowska ◽  
Justyna Kajdanowicz ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Microbial Consortium ◽  
In Vitro Degradation ◽  
Multi Stage ◽  
Polycyclic Aromatic ◽  
Enhanced Bioremediation ◽  
Contaminated Area

The main objectives of this study were to isolate bacteria from soil chronically contaminated with polycyclic aromatic hydrocarbons (PAHs), develop an autochthonous microbial consortium, and evaluate its ability to degrade PAHs in their native contaminated soil. Strains with the best bioremediation potential were selected during the multi-stage isolation process. Moreover, to choose bacteria with the highest bioremediation potential, the presence of PAH-degrading genes (pahE) was confirmed and the following tests were performed: tolerance to heavy metals, antagonistic behavior, phytotoxicity, and antimicrobial susceptibility. In vitro degradation of hydrocarbons led to the reduction of the total PAH content by 93.5% after the first day of incubation and by 99.22% after the eighth day. Bioremediation experiment conducted in situ in the contaminated area resulted in the average reduction of the total PAH concentration by 33.3% after 5 months and by over 72% after 13 months, compared to the concentration recorded before the intervention. Therefore, this study implicates that the development of an autochthonous microbial consortium isolated from long-term PAH-contaminated soil has the potential to enhance the bioremediation process.

scholarly journals Long-term simulation of in situ biostimulation of polycyclic aromatic hydrocarbon-contaminated soil

2012 ◽  
Vol 23 (4) ◽  
pp. 621-633 ◽  
Author(s):  
Stephen D. Richardson ◽  
Maiysha D. Jones ◽  
David R. Singleton ◽  
Michael D. Aitken
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Contaminated Soil ◽  
Polycyclic Aromatic

scholarly journals Looking For Polycyclic Aromatic Hydrocarbon Metabolizing Microorganisms In The Oral Cavity of Smokers

2021 ◽  
Author(s):  
Lin Tao ◽  
M. Paul Chiarelli ◽  
Sylvia I. Pavlova ◽  
Joel L. Schwartz ◽  
James V. DeFrancesco ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Large Scale ◽  
Soil Microbes ◽  
Polycyclic Aromatic ◽  
Pah Exposure ◽  
Resistant Strains ◽  
Oral Microbes ◽  
Bacteria And Fungi

Abstract Certain soil microbes resist and metabolize polycyclic aromatic hydrocarbons (PAHs). The same is true for certain skin microbes. Oral microbes have the potential to oxidize tobacco PAHs to increase their ability to cause cancer. We hypothesized that oral microbes that resist high levels of PAH in smokers exist and can be identified based on their resistance to PAHs. We isolated bacteria and fungi that survived long term in minimal media with PAHs as the sole carbon source from the oral cavity in 11 of 14 smokers and only 1 of 6 nonsmokers. Of bacteria genera that included species that survived harsh PAH exposure in vitro, all were found at trace levels on the oral mucosa, except for Staphylococcus and Actinomyces. Two PAH-resistant strains of Candida albicans (C. albicans) were isolated from smokers. C. albicans is found orally at high levels in tobacco users and some Candida species can metabolize PAHs. The two C. albicans strains were tested for metabolism of two model PAH substrates, pyrene and phenanthrene. The result showed that the PAH-resistant C. albicans strains did not metabolize the two PAHs. In conclusion, evidence for large scale oral microbial metabolism of tobacco PAHs by common oral microbes remains lacking.

scholarly journals Multi-Stage Redox Systems Based on Dicationic P-Containing Polycyclic Aromatic Hydrocarbons

2019 ◽  
Author(s):  
Thomas Delouche ◽  
Antoine Vacher ◽  
Elsa Caytan ◽  
Thierry Roisnel ◽  
Boris Le Guennic ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Organic Radical ◽  
Electrochemical Studies ◽  
Redox Systems ◽  
Redox States ◽  
Multi Stage ◽  
Polycyclic Aromatic ◽  
The Impact

In this communication, we report the straightforward<br>synthesis of unprecedented electron-acceptors based on dicationic P-containing PAHs (Polycyclic Aromatic Hydrocarbons) where two phosphoniums are connected through various PAHs backbones. The impact of pi-extension on both the optical and redox properties is investigated using a joint experimental/theoretical approach.<br>Finally, (spectro)-electrochemical studies prove that these<br>compounds possess three redox states and EPR studies confirms the in situ formation of an organic radical.

In situ generation of human brain organoids on a micropillar array

Lab on a Chip ◽  
2017 ◽  
Vol 17 (17) ◽  
pp. 2941-2950 ◽  
Author(s):  
Yujuan Zhu ◽  
Li Wang ◽  
Hao Yu ◽  
Fangchao Yin ◽  
Yaqing Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Brain ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
In Situ Generation ◽  
Induced Pluripotent ◽  
High Throughput Manner

We present a simple and high throughput manner to generate brain organoids in situ from human induced pluripotent stem cells on micropillar arrays and to investigate long-term brain organogenesis in 3D culture in vitro.

Adaptation to calcium deprivation in the rat: effects of parathyroidectomy.

1977 ◽  
Vol 232 (3) ◽  
pp. E336
Author(s):  
J T Pento ◽  
L C Waite ◽  
P J Tracy ◽  
A D Kenny
Keyword(s):  
Adaptive Response ◽  
Calcium Transport ◽  
Parathyroid Tissue ◽  
Adaptation Period ◽  
Short Term ◽  
High Calcium

The role of parathyroid hormone (PTH) in the adaptive response in gut calcium transport to calcium deprivation has been studied in the rat using both the in vitro everted duodenal sac and the in situ ligated duodenal segment technique. Intact or parathyroidectomized (PTX) young rats were placed on a low calcium (0.01%) diet for 7-, 14-, or 21-day adaptation periods and compared with control rats maintained on a high calcium (1.5%) diet. Prior PTX (3 days before the start of the adaptation period) abolished the adaptive response (enhanced calcium transport) induced by calcium deprivation for a 7-day adaptation period, but did not abolish a response after a 21-day period. A 14-day adaptation period gave equivocal results. It is concluded that PTH appears to be necessary for short-term (7-day) adaptation, but not for long-term (21-day) adaptation to calcium deprivation. However, if accessory parathyroid tissue is present, the data could be interpreted differently: the essentiality of PTH for the adaptive response might be independent of the length of the adaptation period. The data also contribute to a possible resolution of the controversy concerning the involvement of PTH in the regulation of intestinal calcium transport in the rat.

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