scholarly journals Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots

2021 ◽  
Vol 7 (4) ◽  
pp. 82
Author(s):  
Sanne Grundvad Boelt ◽  
Lars Melgaard ◽  
Marta Jadwiga Thorbek ◽  
Nadia Sara Jensen MacSween ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Small Molecules ◽  
Dna Extraction ◽  
Neonatal Screening ◽  
Dried Blood Spots ◽  
Protein Extract ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Precision And Accuracy

Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy.

scholarly journals 25-Hydroxyvitamin D concentration and all-cause mortality: the Melbourne Collaborative Cohort Study

2016 ◽  
Vol 20 (10) ◽  
pp. 1775-1784 ◽  
Author(s):  
Alicia K Heath ◽  
Elizabeth J Williamson ◽  
David Kvaskoff ◽  
Allison M Hodge ◽  
Peter R Ebeling ◽  
...  
Keyword(s):  
Cohort Study ◽  
Cox Regression ◽  
Dried Blood Spots ◽  
Inverse Association ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
Excellent Health ◽  
Melbourne Collaborative Cohort Study ◽  
History Of ◽  
25 Hydroxyvitamin D

AbstractObjectiveTo investigate relationships between mortality and circulating 25-hydroxyvitamin D (25(OH)D), 25-hydroxycholecalciferol (25(OH)D3) and 25-hydroxyergocalciferol (25(OH)D2).DesignCase–cohort study within the Melbourne Collaborative Cohort Study (MCCS). We measured 25(OH)D2and 25(OH)D3in archived dried blood spots by LC–MS/MS. Cox regression was used to estimate mortality hazard ratios (HR), with adjustment for confounders.SettingGeneral community.SubjectsThe MCCS included 29 206 participants, who at recruitment in 1990–1994 were aged 40–69 years, had dried blood spots collected and no history of cancer. For the present study we selected participants who died by 31 December 2007 (n2410) and a random sample (sub-cohort,n2996).ResultsThe HR per 25 nmol/l increment in concentration of 25(OH)D and 25(OH)D3were 0·86 (95 % CI 0·78, 0·96;P=0·007) and 0·85 (95 % CI 0·77, 0·95;P=0·003), respectively. Of 5108 participants, sixty-three (1·2 %) had detectable 25(OH)D2; their mean 25(OH)D concentration was 11·9 (95 % CI 7·3, 16·6) nmol/l higher (P<0·001). The HR for detectable 25(OH)D2was 1·80 (95 % CI 1·09, 2·97;P=0·023); for those with detectable 25(OH)D2, the HR per 25 nmol/l increment in 25(OH)D was 1·06 (95 % CI 0·87, 1·29;Pinteraction=0·02). HR were similar for participants who reported being in good, very good or excellent health four years after recruitment.ConclusionsTotal 25(OH)D and 25(OH)D3concentrations were inversely associated with mortality. The finding that the inverse association for 25(OH)D was restricted to those with no detectable 25(OH)D2requires confirmation in populations with higher exposure to ergocalciferol.

Measurements of 25-Hydroxyvitamin D Concentrations in Archived Dried Blood Spots Are Reliable and Accurately Reflect Those in Plasma

2014 ◽  
Vol 99 (9) ◽  
pp. 3319-3324 ◽  
Author(s):  
Alicia K. Heath ◽  
Elizabeth J. Williamson ◽  
Peter R. Ebeling ◽  
David Kvaskoff ◽  
Darryl W. Eyles ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

scholarly journals Concentration of 25-hydroxyvitamin D from neonatal dried blood spots and the relation to gestational age, birth weight and Ponderal Index: the D-tect study

2018 ◽  
Vol 119 (12) ◽  
pp. 1416-1423 ◽  
Author(s):  
Amélie Keller ◽  
Mina N. Händel ◽  
Peder Frederiksen ◽  
Ramune Jacobsen ◽  
Arieh S. Cohen ◽  
...  
Keyword(s):  
Birth Weight ◽  
Gestational Age ◽  
Dried Blood Spots ◽  
Ponderal Index ◽  
Inverse Association ◽  
Anthropometric Measures ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Gestational Age At Birth

AbstractStudies have suggested that vitamin D status at birth may be associated with a range of neonatal outcomes. The aim of this study was to assess the association between neonatal 25-hydroxyvitamin D3(25(OH)D3) concentration and gestational age, birth weight, Ponderal Index and size for gestational age. Neonatal capillary blood stored as dried blood spots was used to assess 25(OH)D3concentrations among 2686 subjects selected from a random population sub-sample of individuals, born in Denmark from 1 May 1981 to 31 December 2002. There was an inverse association between 25(OH)D3concentration and gestational age at birth of −0·006 (95 % CI −0·009, −0·003,P<0·001) weeks of gestation per 1 nmol/l increase in 25(OH)D3concentration. An inverted U-shaped association between 25(OH)D3and birth weight and Ponderal Index (P=0·04) was found, but no association with size for gestational age was shown. This study suggests that neonatal 25(OH)D3concentration is associated with anthropometric measures at birth known to be correlated with many subsequent health outcomes such as obesity and type 2 diabetes.

Quantitation of 25-hydroxyvitamin D in dried blood spots by 2D LC-MS/MS without derivatization and correlation with serum in adult and pediatric studies

2018 ◽  
Vol 481 ◽  
pp. 61-68 ◽  
Author(s):  
Berit P. Jensen ◽  
Rajneeta Saraf ◽  
Jing Ma ◽  
Sarah Berry ◽  
Cameron C. Grant ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

scholarly journals Response to “importance of C-3 epimer of 25-hydroxyvitamin D in dried blood spots of neonatal population”

2015 ◽  
Vol 137 (3) ◽  
pp. 751-751
Author(s):  
Parveen Bhatti ◽  
David R. Doody ◽  
Roberta Mckean-Cowdin ◽  
Beth A. Mueller
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Neonatal Population

Simplified 25-Hydroxyvitamin D Standardization and Optimization in Dried Blood Spots by LC-MS/MS

2017 ◽  
Vol 100 (5) ◽  
pp. 1328-1336 ◽  
Author(s):  
Andrew J Makowski ◽  
John A Rathmacher ◽  
Ronald L Horst ◽  
Christopher T Sempos
Keyword(s):  
Vitamin D ◽  
Dried Blood Spots ◽  
Serum Concentrations ◽  
Vitamin D Binding Protein ◽  
Serum Samples ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Sample Representation ◽  
Concentration Assessment

Abstract Previous studies have assessed vitamin D status based on the 25-hydroxyvitamin D [25(OH)D] concentration measured in samples from dried blood spots (DBSs). In 40 individuals participating in a clinical study, we compared 25(OH)D levels measured from DBSs and in serum using an LC-MS/MS reference procedure in collaboration with the Vitamin D Standardization Program. The main objective was to simplify and optimize current methods to produce an assay that can be used as a screening tool for 25(OH)D concentration assessment without derivatization. The DBS 25(OH)D levels, compared to serum concentrations, were found to have 101% accuracy overall, and the correlation coefficient (r) was 0.83 (P &lt; 0.0001), with a significant linear relationship. Free 25(OH)D and vitamin D binding protein (VDBP) were assessed in the serum samples for potential correlations to the DBS calculations: the levels of free 25(OH)D had moderate to strong correlation to DBS and serum concentrations, with r values of 0.67 (P &lt; 0.0001) and 0.76 (P &lt; 0.0001), respectively. VDBP and hematocrit had no significant correlation to either DBS or serum sample types, with r values &lt;0.1. In conclusion, the useof two DBSs and an increase in DBS sample size improved overall sample representation without the need for derivatization, and produced an accurate and robust method that can be used to screen 25(OH)D levels.

Evaluation of the Analytical and Clinical Concordance of 25-Hydroxyvitamin D Levels in Dried Blood Spots, Dried Serum Spots, and Serum as Potential Biorepository Specimens

2017 ◽  
Vol 15 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Gellert Karvaly ◽  
Györgyi Molnár-Világos ◽  
Krisztián Kovács ◽  
Katalin Mészáros ◽  
Attila Patócs ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

scholarly journals Environmental and individual predictors of 25-hydroxyvitamin D concentrations in Denmark measured from neonatal dried blood spots: the D-tect study

2019 ◽  
Vol 121 (5) ◽  
pp. 567-575 ◽  
Author(s):  
Amélie Keller ◽  
Peder Frederiksen ◽  
Mina N. Händel ◽  
Ramune Jacobsen ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Maternal Education ◽  
Dried Blood Spots ◽  
Individual Characteristics ◽  
Smoking During Pregnancy ◽  
Aggregated Data ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
Sunshine Hours ◽  
25 Hydroxyvitamin D

AbstractEnvironmental factors such as sunshine hours, temperature and UV radiation (UVR) are known to influence seasonal fluctuations in vitamin D concentrations. However, currently there is poor understanding regarding the environmental factors or individual characteristics that best predict neonatal 25-hydroxyvitamin D (25(OH)D) concentrations. The aims of this study were to (1) identify environmental and individual determinants of 25(OH)D concentrations in newborns and (2) investigate whether environmental factors and individual characteristics could be used as proxy measures for neonatal 25(OH)D concentrations. 25-Hydroxyvitamin D3 (25(OH)D3) was measured from neonatal dried blood spots (DBS) of 1182 individuals born between 1993 and 2002. Monthly aggregated data on daily number of sunshine hours, temperature and UVR, available from 1993, were retrieved from the Danish Meteorological Institute. The individual predictors were obtained from the Danish National Birth register, and Statistics Denmark. The optimal model to predict 25(OH)D3 concentrations from neonatal DBS was the one including the following variables: UVR, temperature, maternal education, maternal smoking during pregnancy, gestational age at birth and parity. This model explained 30 % of the variation of 25(OH)D3 in the neonatal DBS. Ambient UVR in the month before the birth month was the best single-item predictor of neonatal 25(OH)D3, accounting for 24 % of its variance. Although this prediction model cannot substitute for actual blood measurements, it might prove useful in cohort studies ranking individuals in groups according to 25(OH)D3 status.

scholarly journals Comparison of 25‐hydroxyvitamin D concentration in chimpanzee dried blood spots and serum

2020 ◽  
Vol 49 (2) ◽  
pp. 299-306
Author(s):  
Sophie Moittié ◽  
Peter A. Graham ◽  
Nicola Barlow ◽  
Phillipa Dobbs ◽  
Matyas Liptovszky ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D
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