scholarly journals Concentration of 25-hydroxyvitamin D from neonatal dried blood spots and the relation to gestational age, birth weight and Ponderal Index: the D-tect study

2018 ◽  
Vol 119 (12) ◽  
pp. 1416-1423 ◽  
Author(s):  
Amélie Keller ◽  
Mina N. Händel ◽  
Peder Frederiksen ◽  
Ramune Jacobsen ◽  
Arieh S. Cohen ◽  
...  
Keyword(s):  
Birth Weight ◽  
Gestational Age ◽  
Dried Blood Spots ◽  
Ponderal Index ◽  
Inverse Association ◽  
Anthropometric Measures ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Gestational Age At Birth

AbstractStudies have suggested that vitamin D status at birth may be associated with a range of neonatal outcomes. The aim of this study was to assess the association between neonatal 25-hydroxyvitamin D3(25(OH)D3) concentration and gestational age, birth weight, Ponderal Index and size for gestational age. Neonatal capillary blood stored as dried blood spots was used to assess 25(OH)D3concentrations among 2686 subjects selected from a random population sub-sample of individuals, born in Denmark from 1 May 1981 to 31 December 2002. There was an inverse association between 25(OH)D3concentration and gestational age at birth of −0·006 (95 % CI −0·009, −0·003,P<0·001) weeks of gestation per 1 nmol/l increase in 25(OH)D3concentration. An inverted U-shaped association between 25(OH)D3and birth weight and Ponderal Index (P=0·04) was found, but no association with size for gestational age was shown. This study suggests that neonatal 25(OH)D3concentration is associated with anthropometric measures at birth known to be correlated with many subsequent health outcomes such as obesity and type 2 diabetes.

scholarly journals 25-Hydroxyvitamin D concentration and all-cause mortality: the Melbourne Collaborative Cohort Study

2016 ◽  
Vol 20 (10) ◽  
pp. 1775-1784 ◽  
Author(s):  
Alicia K Heath ◽  
Elizabeth J Williamson ◽  
David Kvaskoff ◽  
Allison M Hodge ◽  
Peter R Ebeling ◽  
...  
Keyword(s):  
Cohort Study ◽  
Cox Regression ◽  
Dried Blood Spots ◽  
Inverse Association ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
Excellent Health ◽  
Melbourne Collaborative Cohort Study ◽  
History Of ◽  
25 Hydroxyvitamin D

AbstractObjectiveTo investigate relationships between mortality and circulating 25-hydroxyvitamin D (25(OH)D), 25-hydroxycholecalciferol (25(OH)D3) and 25-hydroxyergocalciferol (25(OH)D2).DesignCase–cohort study within the Melbourne Collaborative Cohort Study (MCCS). We measured 25(OH)D2and 25(OH)D3in archived dried blood spots by LC–MS/MS. Cox regression was used to estimate mortality hazard ratios (HR), with adjustment for confounders.SettingGeneral community.SubjectsThe MCCS included 29 206 participants, who at recruitment in 1990–1994 were aged 40–69 years, had dried blood spots collected and no history of cancer. For the present study we selected participants who died by 31 December 2007 (n2410) and a random sample (sub-cohort,n2996).ResultsThe HR per 25 nmol/l increment in concentration of 25(OH)D and 25(OH)D3were 0·86 (95 % CI 0·78, 0·96;P=0·007) and 0·85 (95 % CI 0·77, 0·95;P=0·003), respectively. Of 5108 participants, sixty-three (1·2 %) had detectable 25(OH)D2; their mean 25(OH)D concentration was 11·9 (95 % CI 7·3, 16·6) nmol/l higher (P<0·001). The HR for detectable 25(OH)D2was 1·80 (95 % CI 1·09, 2·97;P=0·023); for those with detectable 25(OH)D2, the HR per 25 nmol/l increment in 25(OH)D was 1·06 (95 % CI 0·87, 1·29;Pinteraction=0·02). HR were similar for participants who reported being in good, very good or excellent health four years after recruitment.ConclusionsTotal 25(OH)D and 25(OH)D3concentrations were inversely associated with mortality. The finding that the inverse association for 25(OH)D was restricted to those with no detectable 25(OH)D2requires confirmation in populations with higher exposure to ergocalciferol.

scholarly journals Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots

2021 ◽  
Vol 7 (4) ◽  
pp. 82
Author(s):  
Sanne Grundvad Boelt ◽  
Lars Melgaard ◽  
Marta Jadwiga Thorbek ◽  
Nadia Sara Jensen MacSween ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Small Molecules ◽  
Dna Extraction ◽  
Neonatal Screening ◽  
Dried Blood Spots ◽  
Protein Extract ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Precision And Accuracy

Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy.

scholarly journals Large Retrospective Cohort Study of the Association Between Maternal 25-Hydroxyvitamin D Status and Birth Weight of Neonate

2020 ◽  
Author(s):  
Li Yu ◽  
Hai-Jin Ke ◽  
Di Che ◽  
Yong Guo ◽  
Jie-Ling Wu
Keyword(s):  
Vitamin D ◽  
Cohort Study ◽  
Birth Weight ◽  
Gestational Age ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Maternal Vitamin ◽  
Hydroxyvitamin D ◽  
Vitamin D Levels ◽  
25 Hydroxyvitamin D

Abstract Background: The effect of maternal vitamin D status on the birth weight of offspring is controversial as the results are inconsistent between different populations. This large retrospective cohort study aimed to assess the relationship between maternal vitamin D levels and birth weight of neonate. Methods: Serum samples were collected from 10,586 Chinese women in the 2 nd trimester of pregnancy, and the 25-hydroxyvitamin D [25(OH)D] level of the participants was assessed. Using the INTERGROWTH-21st standards, the offsprings were classified into three groups based on their gestational age and birth weight, which were as follows: small for gestational age (SGA), appropriate for gestational age (AGA), and large for gestational age (LGA). Results: The average maternal vitamin D concentration was 61.1 nmol/L. The 25(OH)D concentrations were <75.0, <50.0, and <25.0 nmol/L in 76.6%, 31.1%, and 1.6% of the participants, respectively. Approximately 6.4%, 86.4%, and 7.2% of women delivered SGA, AGA, and LGA infants, respectively. No significant differences were observed in vitamin D levels between the three groups (P = 0.562). With the increase in 25(OH)D levels, the risk of SGA and LGA tended to increase and decrease, respectively. However, the results were not precise. AGA was not affected by 25(OH)D levels. The results of the curve fitting and threshold effect analyses did not support the correlation between vitamin D levels and SGA or LGA. Based on the univariate prediction model and the model that adjusted for the risk factors, the area under the curve was extremely small. Thus, 25(OH)D level is not an effective predictor of SGA and LGA. Conclusions: Low maternal vitamin D levels were not associated with SGA or LGA.

Measurements of 25-Hydroxyvitamin D Concentrations in Archived Dried Blood Spots Are Reliable and Accurately Reflect Those in Plasma

2014 ◽  
Vol 99 (9) ◽  
pp. 3319-3324 ◽  
Author(s):  
Alicia K. Heath ◽  
Elizabeth J. Williamson ◽  
Peter R. Ebeling ◽  
David Kvaskoff ◽  
Darryl W. Eyles ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

Quantitation of 25-hydroxyvitamin D in dried blood spots by 2D LC-MS/MS without derivatization and correlation with serum in adult and pediatric studies

2018 ◽  
Vol 481 ◽  
pp. 61-68 ◽  
Author(s):  
Berit P. Jensen ◽  
Rajneeta Saraf ◽  
Jing Ma ◽  
Sarah Berry ◽  
Cameron C. Grant ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D

Free thyroxin measured in dried blood spots from normal, low-birth-weight, and hypothyroid neonates

1991 ◽  
Vol 37 (12) ◽  
pp. 2114-2117 ◽  
Author(s):  
Françoise Lemonnier ◽  
Joëlle Masson ◽  
Dominique Laroche ◽  
Josette Travert ◽  
Georges Travert
Keyword(s):  
Birth Weight ◽  
Linear Regression ◽  
Low Birth Weight ◽  
Multiple Linear Regression ◽  
Gestational Age ◽  
Neonatal Screening ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Significant Difference ◽  
The Mean

Abstract We have adapted a new radioimmunoassay for free thyroxin (FT4) measurement in dried blood spots for use in neonatal screening for hypothyroidism. The method is easy, fast, and cheap. Within-assay and between-assay CVs are respectively 9.6% and 13.2%. In 997 neonates three days postpartum with normal thyrotropin concentrations, the mean FT4 concentration was 27.2 pmol/L (SD 7.3 pmol/L). There was no significant difference in mean FT4 concentration between boys and girls. FT4 concentrations increased linearly with birth weight or with gestational age, as expressed by multiple linear regression: FT4 (pmol/L) = 0.0016 birth weight (g) + 0.6931 gestational age (weeks) − 4.8772. Only gestational age significantly affected the FT4 value. For five hypothyroid infants tested on day three postpartum, FT4 values were all below the 1st percentile of values from healthy neonates. Thus, when the neonatal concentration of thyrotropin is above normal, FT4 measured in the same sample can provide a reliable earlier diagnosis of hypothyroidism.

scholarly journals Response to “importance of C-3 epimer of 25-hydroxyvitamin D in dried blood spots of neonatal population”

2015 ◽  
Vol 137 (3) ◽  
pp. 751-751
Author(s):  
Parveen Bhatti ◽  
David R. Doody ◽  
Roberta Mckean-Cowdin ◽  
Beth A. Mueller
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Neonatal Population

Simplified 25-Hydroxyvitamin D Standardization and Optimization in Dried Blood Spots by LC-MS/MS

2017 ◽  
Vol 100 (5) ◽  
pp. 1328-1336 ◽  
Author(s):  
Andrew J Makowski ◽  
John A Rathmacher ◽  
Ronald L Horst ◽  
Christopher T Sempos
Keyword(s):  
Vitamin D ◽  
Dried Blood Spots ◽  
Serum Concentrations ◽  
Vitamin D Binding Protein ◽  
Serum Samples ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Sample Representation ◽  
Concentration Assessment

Abstract Previous studies have assessed vitamin D status based on the 25-hydroxyvitamin D [25(OH)D] concentration measured in samples from dried blood spots (DBSs). In 40 individuals participating in a clinical study, we compared 25(OH)D levels measured from DBSs and in serum using an LC-MS/MS reference procedure in collaboration with the Vitamin D Standardization Program. The main objective was to simplify and optimize current methods to produce an assay that can be used as a screening tool for 25(OH)D concentration assessment without derivatization. The DBS 25(OH)D levels, compared to serum concentrations, were found to have 101% accuracy overall, and the correlation coefficient (r) was 0.83 (P &lt; 0.0001), with a significant linear relationship. Free 25(OH)D and vitamin D binding protein (VDBP) were assessed in the serum samples for potential correlations to the DBS calculations: the levels of free 25(OH)D had moderate to strong correlation to DBS and serum concentrations, with r values of 0.67 (P &lt; 0.0001) and 0.76 (P &lt; 0.0001), respectively. VDBP and hematocrit had no significant correlation to either DBS or serum sample types, with r values &lt;0.1. In conclusion, the useof two DBSs and an increase in DBS sample size improved overall sample representation without the need for derivatization, and produced an accurate and robust method that can be used to screen 25(OH)D levels.

Evaluation of the Analytical and Clinical Concordance of 25-Hydroxyvitamin D Levels in Dried Blood Spots, Dried Serum Spots, and Serum as Potential Biorepository Specimens

2017 ◽  
Vol 15 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Gellert Karvaly ◽  
Györgyi Molnár-Világos ◽  
Krisztián Kovács ◽  
Katalin Mészáros ◽  
Attila Patócs ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D
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