scholarly journals Insulin-Like Peptide and FoxO Mediate the Trehalose Catabolism Enhancement during the Diapause Termination Period in the Chinese Oak Silkworm (Antheraea pernyi)

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 784
Author(s):  
Ya-Na Li ◽  
Xiao-Bing Ren ◽  
Zhi-Chao Liu ◽  
Bo Ye ◽  
Zhen-Jun Zhao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Bovine Insulin ◽  
Antheraea Pernyi ◽  
Diapause Termination ◽  
Forkhead Box ◽  
Trehalose Metabolism ◽  
Growth Factor Signalling ◽  
Membrane Bound ◽  
Trehalose Accumulation ◽  
Termination Process

In insects, trehalose accumulation is associated with the insulin/insulin-like growth factor signalling (IIS) pathway. However, whether insulin-like peptide is involved in the regulation of the trehalose metabolism during diapause termination remains largely unknown. This study assessed whether insulin-like peptide (ApILP) enhances the trehalose catabolism in the pupae of Antheraea pernyi during their diapause termination process. Injection of 10 μg of bovine insulin triggered diapause termination and synchronous adult eclosion in diapausing pupae. Moreover, treatment with bovine insulin increased the expression of trehalase 1A (ApTre-1A) and trehalase 2 (ApTre-2), as well as the activity of soluble and membrane-bound trehalase, resulting in a decline in trehalose levels in the haemolymph. Silencing ApILP via RNA interference significantly suppressed the expression of ApTre-1A and ApTre-2, thus leading to an increase in the trehalose concentration during diapause termination. However, neither injection with bovine insulin nor ApILP knockdown directly affected trehalase 1B (ApTre-1B) expression. Moreover, overexpression of the transcription factor forkhead box O (ApFoxO) induced an increase in trehalose levels during diapause termination; however, depletion of ApFoxO accelerated the breakdown of trehalose in diapausing pupae by increasing the expression of ApTre-1A and ApTre-2. The results of this study help to understand the contributions of ApILP and ApFoxO to the trehalose metabolism during diapause termination.

scholarly journals The Modulation of Trehalose Metabolism by 20-Hydroxyecdysone in Antheraea pernyi (Lepidoptera: Saturniidae) During its Diapause Termination and Post-Termination Period

2020 ◽  
Vol 20 (5) ◽  
Author(s):  
Ya-Na Li ◽  
Yu-Bo Liu ◽  
Xue-Qin Xie ◽  
Jia-Ning Zhang ◽  
Wen-Li Li
Keyword(s):  
Adult Development ◽  
Receptor Gene ◽  
Mrna Level ◽  
Adult Emergence ◽  
Dependent Manner ◽  
Ecdysone Receptor ◽  
Antheraea Pernyi ◽  
Diapause Termination ◽  
Membrane Bound ◽  
Stress Protectant

Abstract Trehalose plays a crucial role in the diapause process of many insects, serving as an energy source and a stress protectant. Trehalose accumulation has been reported in diapause pupae of Antheraea pernyi; however, trehalose metabolic regulatory mechanisms associated with diapause termination remain unclear. Here, we showed that the enhanced trehalose catabolism was associated with an increase in endogenous 20-hydroxyecdysone (20E) in hemolymph of A. pernyi pupae during their diapause termination and posttermination period. Injection of 20E increased the mRNA level of trehalase 1A (ApTre-1A) and trehalase 2 (ApTre-2) of A. pernyi diapause pupae in a dose-dependent manner but did not affect the mRNA level of trehalase 1B (ApTre-1B). Meanwhile, exogenous 20E increased the enzyme activities of soluble and membrane-bound trehalase, leading to a decline in hemolymph trehalose. Conversely, the expression of ApTre-1A and ApTre-2 were down-regulated after the ecdysone receptor gene (ApEcRB1) was silenced by RNA interference or by injection of an ecdysone receptor antagonist cucurbitacin B (CucB), which inhibits the 20E pathway. Moreover, CucB treatment delayed adult emergence, which suggests that ApEcRB1 might be involved in regulating pupal-adult development of A. pernyi by mediating ApTre-1A and ApTre-2 expressions. This study provides an overview of the changes in the expression and activity of different trehalase enzymes in A. pernyi in response to 20E, confirming the important role of 20E in controlling trehalose catabolism during A. pernyi diapause termination and posttermination period.

Knockdown of transcription factor forkhead box O3 (FOXO3) suppresses erythroid differentiation in human cells and zebrafish

2015 ◽  
Vol 460 (4) ◽  
pp. 923-930 ◽  
Author(s):  
Hai Wang ◽  
Yanming Li ◽  
Sifeng Wang ◽  
Qian Zhang ◽  
Jiawen Zheng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Erythroid Differentiation ◽  
Human Cells ◽  
Forkhead Box

scholarly journals Expression of the Forkhead box transcription factor Foxo3a in human periapical granulomas

2018 ◽  
Vol 60 (4) ◽  
pp. 479-483
Author(s):  
Kae Ishii ◽  
Keisuke Hatori ◽  
Osamu Takeichi ◽  
Kosuke Makino ◽  
Kazuma Himi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Forkhead Box

scholarly journals Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations

Open Biology ◽  
2012 ◽  
Vol 2 (7) ◽  
pp. 120091 ◽  
Author(s):  
Matthew D. Rolfe ◽  
Andrea Ocone ◽  
Melanie R. Stapleton ◽  
Simon Hall ◽  
Eleanor W. Trotter ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Systems Analysis ◽  
Kinetic Modelling ◽  
Statistical Modelling ◽  
Spatial Effects ◽  
Transcript Profile ◽  
Terminal Oxidases ◽  
Adaptive Processes ◽  
Membrane Bound ◽  
Transcript Profiles

Understanding gene regulation requires knowledge of changes in transcription factor (TF) activities. Simultaneous direct measurement of numerous TF activities is currently impossible. Nevertheless, statistical approaches to infer TF activities have yielded non-trivial and verifiable predictions for individual TFs. Here, global statistical modelling identifies changes in TF activities from transcript profiles of Escherichia coli growing in stable (fixed oxygen availabilities) and dynamic (changing oxygen availability) environments. A core oxygen-responsive TF network, supplemented by additional TFs acting under specific conditions, was identified. The activities of the cytoplasmic oxygen-responsive TF, FNR, and the membrane-bound terminal oxidases implied that, even on the scale of the bacterial cell, spatial effects significantly influence oxygen-sensing. Several transcripts exhibited asymmetrical patterns of abundance in aerobic to anaerobic and anaerobic to aerobic transitions. One of these transcripts, ndh , encodes a major component of the aerobic respiratory chain and is regulated by oxygen-responsive TFs ArcA and FNR. Kinetic modelling indicated that ArcA and FNR behaviour could not explain the ndh transcript profile, leading to the identification of another TF, PdhR, as the source of the asymmetry. Thus, this approach illustrates how systematic examination of regulatory responses in stable and dynamic environments yields new mechanistic insights into adaptive processes.

Novel role of forkhead box O 4 transcription factor in cancer: Bringing out the good or the bad

2018 ◽  
Vol 50 ◽  
pp. 1-12 ◽  
Author(s):  
Shuai Jiang ◽  
Zhi Yang ◽  
Shouyin Di ◽  
Wei Hu ◽  
Zhiqiang Ma ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Forkhead Box
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