Frequent Alteration of Annexin A9 and A10 in HPV-Negative Head and Neck Squamous Cell Carcinomas: Correlation with the Histopathological Differentiation Grade

The annexin protein superfamily has been implicated in multiple physiological and pathological processes, including carcinogenesis. Altered expression of various annexins has frequently been observed and linked to the development and progression of various human malignancies. However, information is lacking on the expression and clinical significance of annexin A9 (ANXA9) and A10 (ANXA10) in head and neck squamous cell carcinomas (HNSCC). ANXA9 and ANXA10 expression was evaluated in a large cohort of 372 surgically treated HPV-negative HNSCC patients and correlated with the clinicopathologic parameters and disease outcomes. Down-regulation of ANXA9 expression was found in 42% of HNSCC tissue samples, compared to normal epithelia. ANXA9 expression in tumors was significantly associated with oropharyngeal location and histological differentiation grade (P < 0.001). In marked contrast, ANXA10 expression was absent in normal epithelium, but variably detected in the cytoplasm of cancer cells. Positive ANXA10 expression was found in 64% of tumors, and was significantly associated with differentiation grade (P < 0.001), being also more frequent in oropharyngeal tumors (P = 0.019). These results reveal that the expression of both ANXA9 and ANXA10 is frequently altered in HNSCC and associated to the tumor differentiation grade, suggesting that they could be implicated in the pathogenesis of these cancers.

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809 Poster Frequency and types of TP53 mutations in squamous cell carcinomas of the head and neck — a comparison of two methods used on formalin fixed paraffin embedded tissue samples

Radiotherapy and Oncology ◽

10.1016/s0167-8140(02)83118-8 ◽

2002 ◽

Vol 64 ◽

pp. S241

Keyword(s):

Head And Neck ◽

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Tissue Samples ◽

Tp53 Mutations ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Altered expression and activity of G1/S cyclins and cyclin-dependent kinases characterize squamous cell carcinomas of the head and neck

International Journal of Cancer ◽

10.1002/(sici)1097-0215(19971114)73:4<551::aid-ijc16>3.0.co;2-c ◽

1997 ◽

Vol 73 (4) ◽

pp. 551-555 ◽

Cited By ~ 27

Author(s):

Vyomesh Patel ◽

Judit Jakus ◽

Candice M. Harris ◽

John F. Ensley ◽

Keith C. Robbins ◽

...

Keyword(s):

Head And Neck ◽

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Altered Expression ◽

Cyclin Dependent Kinases ◽

Expression And Activity

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Single Tissue Samples from Head and Neck Squamous Cell Carcinomas are Representative Regarding the Entire Tumor’s Chemosensitivity to Cisplatin and Docetaxel

Onkologie ◽

10.1159/000209148 ◽

2009 ◽

Vol 32 (5) ◽

pp. 7-7 ◽

Cited By ~ 10

Author(s):

Gunnar Wichmann ◽

Iris-Susanne Horn ◽

Andreas Boehm ◽

Christian Mozet ◽

Katrin Tschöp ◽

...

Keyword(s):

Head And Neck ◽

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Tissue Samples

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Nuclear DNA content using computerized image cytometry of squamous cell carcinomas of the head and neck

The Journal of Laryngology & Otology ◽

10.1017/s0022215100136394 ◽

1997 ◽

Vol 111 (1) ◽

pp. 43-47 ◽

Cited By ~ 6

Author(s):

S. K. Sarker ◽

K. Ghufoor ◽

K. S. Patel ◽

N. S. Tolley ◽

D. V. Coleman

Keyword(s):

Flow Cytometry ◽

Head And Neck ◽

Squamous Cell ◽

Nuclear Dna ◽

Clinical Stage ◽

Nuclear Dna Content ◽

Image Cytometry ◽

Squamous Cell Carcinomas ◽

Histological Differentiation ◽

Ploidy Status

AbstractPloidy status using flow cytometry of head and neck cancers may be of prognostic value. We describe the use of image cytometry in ploidy measurement of squamous cell carcinomas of the head and neck (SCCHN). This technique allows only tumour cells to be measured, thereby rejecting debris, artefact and benign cells. Tissue sections were cut from tumours and then Feulgen stained. A total of 60 patients were included in this study, 23 females and 37 males. The data reveals a relationship between ploidy status and the histological differentiation. However, the ploidy status and histological differentiation do not appear to correlate to the clinical stage of the disease. This method of measuring ploidy may be more accurate than flow cytometry and may have a prognostic role in head and neck cancer patients. A study comparing both methods may demonstrate this and we aim to evaluate this in the future.

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Transient Receptor Potential (TRP) Channels in Head-and-Neck Squamous Cell Carcinomas: Diagnostic, Prognostic, and Therapeutic Potentials

International Journal of Molecular Sciences ◽

10.3390/ijms21176374 ◽

2020 ◽

Vol 21 (17) ◽

pp. 6374

Author(s):

Fruzsina Kiss ◽

Krisztina Pohóczky ◽

Arpad Szállási ◽

Zsuzsanna Helyes

Keyword(s):

Early Diagnosis ◽

Head And Neck ◽

Squamous Cell ◽

Transient Receptor Potential ◽

Trp Channels ◽

Receptor Potential ◽

Squamous Cell Carcinomas ◽

Normal Epithelium ◽

Channel Expression ◽

Transient Receptor

Head-and-neck squamous cell carcinomas (HNSCC) remain a leading cause of cancer morbidity and mortality worldwide. This is a largely preventable disease with smoking, alcohol abuse, and human papilloma virus (HPV) being the main risk factors. Yet, many patients are diagnosed with advanced disease, and no survival improvement has been seen for oral SCC in the past decade. Clearly, new diagnostic and prognostic markers are needed for early diagnosis and to guide therapy. Gene expression studies implied the involvement of transient receptor potential (TRP) channels in the pathogenesis of HNSCC. TRPs are expressed in normal epithelium where they play a key role in proliferation and differentiation. There is increasing evidence that the expression of TRP channels may change in HNSCC with important implications for diagnosis, prognosis, and therapy. In this review, we propose that TRP channel expression may afford a novel opportunity for early diagnosis of HNSCC and targeted molecular treatment.

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