scholarly journals In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends

2022 ◽  
Vol 8 (1) ◽  
pp. 75
Author(s):  
Kustrim Cerimi ◽  
Udo Jäckel ◽  
Vera Meyer ◽  
Ugarit Daher ◽  
Jessica Reinert ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Standardized Test ◽  
Submerged Cultivation ◽  
Current Status ◽  
Human Respiratory Tract ◽  
Advantages And Disadvantages ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds

Microbial volatile organic compounds (mVOC) are metabolic products and by-products of bacteria and fungi. They play an important role in the biosphere: They are responsible for inter- and intra-species communication and can positively or negatively affect growth in plants. But they can also cause discomfort and disease symptoms in humans. Although a link between mVOCs and respiratory health symptoms in humans has been demonstrated by numerous studies, standardized test systems for evaluating the toxicity of mVOCs are currently not available. Also, mVOCs are not considered systematically at regulatory level. We therefore performed a literature survey of existing in vitro exposure systems and lung models in order to summarize the state-of-the-art and discuss their suitability for understanding the potential toxic effects of mVOCs on human health. We present a review of submerged cultivation, air-liquid-interface (ALI), spheroids and organoids as well as multi-organ approaches and compare their advantages and disadvantages. Furthermore, we discuss the limitations of mVOC fingerprinting. However, given the most recent developments in the field, we expect that there will soon be adequate models of the human respiratory tract and its response to mVOCs.

scholarly journals Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

2021 ◽  
Vol 7 (1) ◽  
pp. 46
Author(s):  
Warin Intana ◽  
Suchawadee Kheawleng ◽  
Anurag Sunpapao
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Fungal Growth ◽  
Fruit Rot ◽  
Postharvest Disease ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
Volatile Organic

Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

scholarly journals In vitro profiling of volatile organic compounds released by Simpson-Golabi-Behmel syndrome adipocytes

2019 ◽  
Vol 1104 ◽  
pp. 256-261 ◽  
Author(s):  
Paweł Mochalski ◽  
Eva Diem ◽  
Karl Unterkofler ◽  
Axel Mündlein ◽  
Heinz Drexel ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic

Variable emissions of microbial volatile organic compounds (MVOCs) from root-associated fungi isolated from Scots pine

2010 ◽  
Vol 44 (30) ◽  
pp. 3651-3659 ◽  
Author(s):  
Jaana Bäck ◽  
Hermanni Aaltonen ◽  
Heidi Hellén ◽  
Maija K. Kajos ◽  
Johanna Patokoski ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Scots Pine ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds

Detection and purification of microbial volatile organic compounds

2021 ◽  
pp. 51-64
Author(s):  
Jeevanandam Vaishnavi ◽  
Swarnkumar Reddy ◽  
Santhanam Narmadha ◽  
W. Jabez Osborne
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds

Pathogen suppression by microbial volatile organic compounds in soils

2019 ◽  
Vol 95 (8) ◽  
Author(s):  
Wietse de Boer ◽  
Xiaogang Li ◽  
Annelein Meisner ◽  
Paolina Garbeva
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Abiotic Factors ◽  
Management Strategies ◽  
Field Studies ◽  
Growth Media ◽  
Soil Borne Pathogens ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Pathogen Suppression

ABSTRACT There is increasing evidence that microbial volatile organic compounds (mVOCs) play an important role in interactions between microbes in soils. In this minireview, we zoom in on the possible role of mVOCs in the suppression of plant-pathogenic soil fungi. In particular, we have screened the literature to see what the actual evidence is that mVOCs in soil atmospheres can contribute to pathogen suppression. Furthermore, we discuss biotic and abiotic factors that influence the production of suppressive mVOCs in soils. Since microbes producing mVOCs in soils are part of microbial communities, community ecological aspects such as diversity and assembly play an important role in the composition of produced mVOC blends. These aspects have not received much attention so far. In addition, the fluctuating abiotic conditions in soils, such as changing moisture contents, influence mVOC production and activity. The biotic and abiotic complexity of the soil environment hampers the extrapolation of the production and suppressing activity of mVOCs by microbial isolates on artificial growth media. Yet, several pathogen suppressive mVOCs produced by pure cultures do also occur in soil atmospheres. Therefore, an integration of lab and field studies on the production of mVOCs is needed to understand and predict the composition and dynamics of mVOCs in soil atmospheres. This knowledge, together with the knowledge of the chemistry and physical behaviour of mVOCs in soils, forms the basis for the development of sustainable management strategies to enhance the natural control of soil-borne pathogens with mVOCs. Possibilities for the mVOC-based control of soil-borne pathogens are discussed.

scholarly journals Detection and Identification of Microbial Volatile Organic Compounds of the Green Mold Disease

2020 ◽  
Vol 11 (2) ◽  
pp. 14-28
Author(s):  
Dalma Radványi ◽  
András Geösel ◽  
Zsuzsa Jókai ◽  
Péter Fodor ◽  
Attila Gere
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Identification System ◽  
Mushroom Species ◽  
Detection And Identification ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Green Mould

Button mushrooms are one of the most commonly cultivated mushroom species facing different risks e.g.: viral, bacterial and fungal diseases. One of the most common problems is caused by Trichoderma aggressivum, or ‘green mould' disease. The presence or absence of mushroom disease-related moulds can sufficiently be detected from the air by headspace solid-phase microextraction coupled gas chromatography-mass spectrometry (HS SPME GC-MS) via their emitted microbial volatile organic compounds (MVOCs). In the present study, HS SPME GC-MS was used to explore the volatile secondary metabolites released by T. aggressivum f. europaeum on different nutrient-rich and -poor media. The MVOC pattern of green mould was determined, then media-dependent and independent biomarkers were also identified during metabolomic experiments. The presented results provide the basics of a green mould identification system which helps producers reducing yield loss, new directions for researchers in mapping the metabolomic pathways of T. aggressivum and new tools for policy makers in mushroom quality control.

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