Cross-Contamination Risk of Dental Tray Adhesives: An In Vitro Study

Background: The aim of this study was to investigate the risk of cross-contamination in dental tray adhesives with reusable brush systems. Methods: Four dental tray adhesives with different disinfectant components were examined for risk as a potential transmission medium for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus oralis, and Candida albicans. Bacterial and fungal strains were mixed with artificial saliva. The contaminated saliva was intentionally added to tray adhesive liquid samples. At baseline and up to 60 min, 100 microliters of each sample were collected and cultivated aerobically on Columbia and Sabouraud agar for 24 or 48 h, respectively. Results: At baseline, contamination with Staphylococcus aureus and Candida albicans could be identified in three out of four adhesives. In the subsequent samples, low counts of up to 20 colony-forming units per milliliter could be observed for Staphylococcus aureus. All other strains did not form colonies at baseline or subsequently. Adhesives with isopropanol or ethyl acetate as disinfectant additives were most effective in preventing contamination, while adhesives with hydrogen chloride or acetone as a disinfectant additive were the least effective. Conclusion: Within 15 min, the tested adhesives appeared to be sufficiently bactericidal and fungicidal against all microorganisms tested.

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The Effect of Cinnamomum burmannii Water Extraction Against Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans: In Vitro Study

Folia Medica Indonesiana ◽

10.20473/fmi.v55i4.24449 ◽

2021 ◽

Vol 55 (4) ◽

pp. 285

Author(s):

Bernadette Dian Novita ◽

Silvia Sutandhio

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Water Extract ◽

In Vitro Study ◽

Antimicrobial Effect ◽

Bark Extract ◽

Native Plant ◽

Cinnamomum Burmannii

Indonesian cinnamon (Cinnamomum burmannii) is a native plant of Indonesia that has a lot of potential. The most consumed part is the bark. This study aims to examine the antimicrobial effect of Cinnamomum burmannii bark extract on various types of pathogenic microbes, namely Staphylococcus aureus, Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans. An experimental study using a water extract (infusion) of Cinnamomum burmannii bark and a microbial test obtained from the Faculty of Pharmacy, Widya Mandala Catholic University, Surabaya. The antimicrobial effect test was carried out by the microdilution method in 96-well-microplate to determine the Minimum Inhibitory Level (MIC) and implantation on solid media to determine the Minimum Kill Rate (KBM). The MIC and KBM against Staphylococcus aureus were 625-1,250 ppm and 1,250-2,500 ppm, respectively. MIC and KBM for Enterobacter spp., Pseudomonas aeruginosa, and Candida albicans were not found at the highest concentrations tested at 10,000 ppm. Cinnamomum burmannii extract can be used as a potential ingredient with antimicrobial effects, especially against Gram-positive bacteria. Future studies should pay attention to the quality of simplicia, particle size, and the most effective extraction methods extracting antimicrobial substances from simplicia

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Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-44.5.5 ◽

2020 ◽

Vol 44 (5) ◽

pp. 315-322

Author(s):

Liz Mariana Hernández-Bautista ◽

Raúl Márquez-Preciado ◽

Marine Ortiz-Magdaleno ◽

Amaury Pozos-Guillén ◽

Saray Aranda-Romo ◽

...

Keyword(s):

In Vitro Study ◽

Inhibitory Effect ◽

Antagonistic Effect ◽

Probiotic Bacteria ◽

Commercial Preparation ◽

Sabouraud Agar ◽

Colony Forming Units ◽

Commercial Probiotics ◽

De Man

Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans. Study design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Serial dilutions of 10−1 to 10−7 were made and viability was verified and quantified. For the antagonism tests, a suspension/inoculum of Lactobacillus strains recovered from each commercial preparation (4–30 × 109) and C. albicans ATCC 90028 (1.5–8 × 108) was prepared in MRS broth and incubated for 48 hours at 36°C, then plated on Dextrose Sabouraud Agar with Chloramphenicol and Rogosa Agar and the colony-forming units (CFU) were quantified. Additionally, viability was evaluated using the LIVE/DEAD® Yeast and Bacterial Viability kit. Results: The probiotic that produced the highest acidity of the medium was Lactipan®, followed by Probiseis® and Liolactil®, while Probio Hp+® showed the least change. Probiseis® was determined to have the highest growth of probiotic bacteria and the highest inhibition on C. albicans, followed by Lactipan®; Liolactil® and ProbioHp+® showed the least effect. In fluorescence tests, ProBiseis® showed the best effect, followed by Liolactil® and Lactipan®; Probio Hp+® had less of an effect. Conclusions: Two commercial products (ProBiseis and Lactipan) whose formulations have L. acidophilus, L. casei, L. rhamnosus, L. plantarum, B. infantis, and S. thermophilus have a greater inhibitory effect on C. albicans ATCC 90028

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An assessment of Antimicrobial Activity of Three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by a Direct Contact Test: An In Vitro Study

10.21203/rs.2.16983/v2 ◽

2020 ◽

Author(s):

Trishnika Chakraborty ◽

Sonali Taneja

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Enterococcus Faecalis ◽

In Vitro Study ◽

Microbial Count ◽

Time Intervals ◽

Ah Plus ◽

Endodontic Sealers ◽

And Staphylococcus Aureus

Abstract For successful endodontic treatment, the antimicrobial property of endodontic filling is mandatory. Hence, the aim of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by direct contact test (DCT).Materials and MethodsThe antimicrobial efficacy of three different sealers, AH Plus, MTA Fillapex and Endo Sequence BC, was tested against Enterococcus faecalis ATCC 29212, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension was drawn and spread over culture plates to determine the colony count using a digital colony counter. Readings were taken at 1 hr (fresh specimen), and then at 24 hrs (after setting). The results were tabulated, and statistical analysis was performed using one-way ANOVA and Tukey’s HSD test.ResultsDCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex and Endoseq BC at both time intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum microbial count for Group 1 (AH Plus) for both time intervals (1 hour and 24 hours).ConclusionEndosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both time intervals, followed by MTA Fillapex and AH Plus.

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An assessment of Antimicrobial Activity of Three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by a Direct Contact Test: An In Vitro Study

10.21203/rs.2.16983/v1 ◽

2019 ◽

Author(s):

Trishnika Chakrabort ◽

Sonali Taneja

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Enterococcus Faecalis ◽

In Vitro Study ◽

Microbial Count ◽

Time Intervals ◽

Ah Plus ◽

Endodontic Sealers ◽

And Staphylococcus Aureus

Abstract For a successful endodontic treatment antimicrobial property of endodontic filling is mandatory. Hence the aim of this in vitro study was to assess the antibacterial efficacy of three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by direct contact test (DCT). Materials and Methods The antimicrobial efficacy of three different sealers (Group 1= AH Plus, 2= MTA Fillapex and 3= Endo Sequence BC) were tested against Enterococcus faecalis ATCC 29212, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter 10 μL of suspension was drawn and spread over cultural plates in order to determine the colony count using digital colony counter. Readings were taken at 1 hr (fresh specimen), then at 24 hrs (after setting). The results were tabulated and statistical analysis was done using one way ANOVA and Tukey HSD test Results DCT showed a significant decrease in microbial count in AH Plus,MTA Fillapex and Endoseq BC at both the time intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum for Group 1(AH Plus) for both the time intervals (1 hour and 24 hours). Conclusion Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the time intervals, followed by MTA Fillapex and AH Plus.

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Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study

International Journal of Prosthodontics and Restorative Dentistry ◽

10.5005/jp-journals-10019-1061 ◽

2012 ◽

Vol 2 (4) ◽

pp. 129-135 ◽

Cited By ~ 1

Author(s):

Prabha Shakya ◽

Deshraj Jain ◽

Tripty Rahangdale

Keyword(s):

Candida Albicans ◽

Oral Candidiasis ◽

In Vitro Study ◽

Acrylic Resin ◽

Vitro Study ◽

Degree Of Contamination ◽

Colony Forming Units ◽

The Mean ◽

Level Of Significance

ABSTRACT Purpose Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin, cold-cured acrylic resin and microwave-cured acrylic resin. Materials and methods A total of 40 specimens of each type of acrylic resin were made; 20 specimens of each type were contaminated before delmopinol treatment and 20 specimens were contaminated after delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μl of each tube was then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 hours at 37oC, in order to check microbial growth. Two-way ANOVA analysis of variance test and posthoc Turkey's test were carried out to ascertain the level of significance (p < 0.001) of various observations. Results Mean of the turbidity degree of contamination after delmopinol application for heat-cured acrylic resin specimens (A1) 1440.80, colony forming units (CFU) (⨯106/ml), cold-cured acrylic resin specimens (B1) 833.30 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C1) 944.70 CFU (⨯106/ml) was significantly higher than the mean of the turbidity degree of contamination before delmopinol application for heatcured acrylic resin specimens (A2) 550.85 CFU (⨯106/ml), coldcured acrylic resin specimens (B2) 341.45 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C2) 451.50 CFU (⨯106/ml). Conclusion In case of contamination after delmopinol application, heat-cured acrylic resin showed maximum reduction in adherence of C. albicans in the study. How to cite this article Shakya P, Jain D, Rahangdale T. Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):129-135.

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Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study

RGO - Revista Gaúcha de Odontologia ◽

10.1590/1981-863720180002000033328 ◽

2018 ◽

Vol 66 (2) ◽

pp. 122-128 ◽

Cited By ~ 1

Author(s):

Maitê Alves PEQUENO ◽

Marissa Rodrigues SILVESTRE ◽

Isabela AMÊNDOLA ◽

Célia Regina Gonçalves e SILVA ◽

Mariella Vieira Pereira LEÃO ◽

...

Keyword(s):

Candida Albicans ◽

Research Study ◽

Growth Control ◽

In Vitro Study ◽

Hydroalcoholic Extract ◽

Violet Crystal ◽

Colony Forming Units ◽

Significant Difference ◽

Inhibition Control

ABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®).

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