Comparative Metabolomics Reveals Fungal Conversion of Co-Existing Bacterial Metabolites within a Synthetic Aspergillus-Streptomyces Community

In nature, secondary metabolites have been proven to be the essential communication media between co-occurring microorganisms and to influence their relationship with each other. In this study, we conducted a metabolomics survey of the secondary metabolites of an artificial co-culture related to a hydrothermal vent fungal–bacterial community comprising Aspergillus sclerotiorum and Streptomyces and their reciprocal relationship. The fungal strain was found to increase the secretion of notoamides and the compound cyclo(Pro-Trp) produced by the actinomycetes strain was discovered to be the responsible molecule. This led to the hypothesis that the fungi transformed cyclo(Pro-Trp) synthesized by the actinomycetes as the biosynthetic precursors of notoamides in the chemical communication. Further analysis showed Streptomyces sp. WU20 was efficient in transforming amino acids into cyclo(Pro-Trp) and adding tryptophan as well as proline into the chemical communication enhanced the induction of the notoamide accumulation. Thus, we propose that the microbial transformation during the synthetic metabolically-mediated chemical communication might be a promising means of speeding up the discovery of novel bioactive molecules. The objective of this research was to clarify the mechanism of microbial transformation for the chemical communication. Besides, this research also highlights the utility of mass spectrometry-based metabolomics as an effective tool in the direct biochemical analysis of community metabolites.

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Metabolomics: towards acceleration of antibacterial plant-based leads discovery

Phytochemistry Reviews ◽

10.1007/s11101-021-09762-4 ◽

2021 ◽

Author(s):

Elwira Sieniawska ◽

Milen I. Georgiev

Keyword(s):

Secondary Metabolites ◽

Plant Secondary Metabolites ◽

Metabolic Stress ◽

Point Of View ◽

Biochemical Networks ◽

Bioactive Molecules ◽

Integrative Approach ◽

Bacterial Cells ◽

Cell Functions ◽

Comparative Metabolomics

AbstractThe wide and not yet fully uncovered potential of plant secondary metabolites make plants a rich source of drug leads. Metabolomics enables the study of the metabolic perturbations taking place in bacterial cells under the influence of plant-based bioactive molecules. It reveals the changes in metabolic pathways within bacteria, reflecting the reprogramming of the biochemical networks. From this point of view, metabolomics is valuable in understanding the alteration of cell functions when bacteria are subjected to metabolic stress caused by treatment with secondary metabolites, that inhibit their growth. In this review the application of metabolomics in revealing bacteria response to plant-derived secondary metabolites is presented. Metabolomics may be a way to select antibacterial plant-based bioactive secondary metabolites and to understand their mode of action. Therefore, herein the usefulness of metabolomic approach in screening for antimicrobials from plants, as well as in exploring the target points in bacteria metabolism and in uncovering the mechanisms of bacteria adaptation and resistance to natural antimicrobials are discussed. Basic chemometrics and molecular networking are successfully applied for the identification of antimicrobial molecules in complex plant mixtures. Determination of antibacterial modes of action is done through classification strategy, pathway analysis and integration of transcriptomics, genomics and metabolomics, whereas, comparative metabolomics and integrative approach is useful in revealing the bacterial mechanisms of resistance.

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Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

Molecules ◽

10.3390/molecules26226789 ◽

2021 ◽

Vol 26 (22) ◽

pp. 6789

Author(s):

Misael Bermúdez-Bazán ◽

Gustavo Adolfo Castillo-Herrera ◽

Judith Esmeralda Urias-Silvas ◽

Antonio Escobedo-Reyes ◽

Mirna Estarrón-Espinosa

Keyword(s):

Secondary Metabolites ◽

Biological Activities ◽

Waste Product ◽

Bioactive Molecules ◽

Alcoholic Beverages ◽

Therapeutic Benefits ◽

Biological Potential ◽

Novel Drugs ◽

Ancient Times

Agaves are plants used in the production of alcoholic beverages and fibers. Ever since ancient times, pre-Hispanic cultures in Mexico have used them in traditional medicine to cure different ailments. Over the years, studies of the active principles responsible for the therapeutic benefits of agaves have increased. Leaves and fibers are the main agro-wastes generated in tequila and mezcal production, while fibers are the main waste product in the textile sector. Different investigations have referred to the agro-waste from agave processing as a source of bioactive molecules called secondary metabolites (SM). Among them, phenols, flavonoids, phytosterols, and saponins have been extracted, identified, and isolated from these plants. The role of these molecules in pest control and the prospect of metabolites with the biological potential to develop novel drugs for chronic and acute diseases represent new opportunities to add value to these agro-wastes. This review aims to update the biological activities and recent applications of the secondary metabolites of the genus Agave.

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Microbial Transformation of Phenazines by Aspergillus Sclerotiorum

Mycologia ◽

10.1080/00275514.1969.12018758 ◽

1969 ◽

Vol 61 (3) ◽

pp. 452-467 ◽

Cited By ~ 1

Author(s):

James C. Hill ◽

George T. Johnson

Keyword(s):

Microbial Transformation ◽

Aspergillus Sclerotiorum

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Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process

Microorganisms ◽

10.3390/microorganisms8091262 ◽

2020 ◽

Vol 8 (9) ◽

pp. 1262

Author(s):

Charifat Said Hassane ◽

Mireille Fouillaud ◽

Géraldine Le Goff ◽

Aimilia D. Sklirou ◽

Jean Bernard Boyer ◽

...

Keyword(s):

Secondary Metabolites ◽

Marine Sponge ◽

Marine Sponges ◽

Sirtuin 1 ◽

Bioactive Molecules ◽

Aging Research ◽

Fyn Kinase ◽

The Indian Ocean ◽

Metabolites Production ◽

Future Work

Aging research aims at developing interventions that delay normal aging processes and some related pathologies. Recently, many compounds and extracts from natural products have been shown to delay aging and/or extend lifespan. Marine sponges and their associated microorganisms have been found to produce a wide variety of bioactive secondary metabolites; however, those from the Southwest of the Indian Ocean are much less studied, especially regarding anti-aging activities. In this study, the microbial diversity of the marine sponge Scopalina hapalia was investigated by metagenomic analysis. Twenty-six bacterial and two archaeal phyla were recovered from the sponge, of which the Proteobacteria phylum was the most abundant. In addition, thirty isolates from S. hapalia were selected and cultivated for identification and secondary metabolites production. The selected isolates were affiliated to the genera Bacillus, Micromonospora, Rhodoccocus, Salinispora, Aspergillus, Chaetomium, Nigrospora and unidentified genera related to the family Thermoactinomycetaceae. Crude extracts from selected microbial cultures were found to be active against seven targets i.e., elastase, tyrosinase, catalase, sirtuin 1, Cyclin-dependent kinase 7 (CDK7), Fyn kinase and proteasome. These results highlight the potential of microorganisms associated with a marine sponge from Mayotte to produce anti-aging compounds. Future work will focus on the isolation and the characterization of bioactive molecules.

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Secondary metabolites ofTuberculariasp. TF5, an endophytic fungal strain ofTaxus mairei

Natural Product Research ◽

10.1080/14786410701852818 ◽

2009 ◽

Vol 23 (1) ◽

pp. 70-76 ◽

Cited By ~ 10

Author(s):

Yaoyao Li ◽

Chunhua Lu ◽

Zhiyu Hu ◽

Yaojian Huang ◽

Yuemao Shen

Keyword(s):

Secondary Metabolites ◽

Fungal Strain

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Cytotoxic Secondary Metabolites from an Endophytic Fungal Strain of Phomopsis phyllanthicola

Chinese Journal of Organic Chemistry ◽

10.6023/cjoc201812009 ◽

2019 ◽

Vol 39 (5) ◽

pp. 1475

Author(s):

Hongxin Liu ◽

Haohua Li ◽

Yuchan Chen ◽

Haibo Tan ◽

Weimin Zhang

Keyword(s):

Secondary Metabolites ◽

Fungal Strain

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Metabolomic Profiling of Burkholderia Cenocepacia in Synthetic Cystic Fibrosis Sputum Media Reveals Nutrient Environment-Specific Production of Virulence Factors

10.21203/rs.3.rs-725834/v1 ◽

2021 ◽

Author(s):

Olakunle A. Jaiyesimi ◽

Andrew C. McAvoy ◽

David N. Fogg ◽

Neha Garg

Keyword(s):

Cystic Fibrosis ◽

Quorum Sensing ◽

Secondary Metabolites ◽

Virulence Factors ◽

Homoserine Lactone ◽

Burkholderia Cenocepacia ◽

Comparative Metabolomics ◽

Nutrient Environment ◽

Life Threatening ◽

Future Work

Abstract Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how the chemical environment of CF lung influences the production of small molecule virulence factors by these strains. Here we compare metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum media (SCFM2) and in a routine laboratory media (LB), in the presence and absence of the antibiotic trimethoprim. Using a mass spectrometry based untargeted metabolomics approach, we identify several compound classes which are differentially produced in SCFM2 compared to LB media, including siderophores, antimicrobials, quorum sensing signals, and various lipids. Furthermore, we describe that specific metabolites are induced by the antibiotic trimethoprim only in SCFM2 when compared to LB. Herein, C13-acyl-homoserine lactone, a quorum sensing signal previously not known to be produced by B. cenocepacia as well as pyochelin-type siderophores were exclusively detected during growth in SCFM2 in the presence of trimethoprim. The comparative metabolomics approach described in this study provides insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggests future work which could identify personalized strain-specific regulatory mechanisms involved in production of secondary metabolites.

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Recent Advances in Understanding and Engineering Polyketide Synthesis

F1000Research ◽

10.12688/f1000research.7326.1 ◽

2016 ◽

Vol 5 ◽

pp. 208 ◽

Cited By ~ 6

Author(s):

Wenjun Zhang ◽

Joyce Liu

Keyword(s):

Biochemical Analysis ◽

Polyketide Synthase ◽

Bioactive Molecules ◽

New Developments ◽

Recent Advances ◽

Fundamental Understanding ◽

Block Formation ◽

Enzymatic Machinery

Polyketides are a diverse group of natural products that form the basis of many important drugs. The engineering of the polyketide synthase (PKS) enzymes responsible for the formation of these compounds has long been considered to have great potential for producing new bioactive molecules. Recent advances in this field have contributed to the understanding of this powerful and complex enzymatic machinery, particularly with regard to domain activity and engineering, unique building block formation and incorporation, and programming rules and limitations. New developments in tools for in vitro biochemical analysis, full-length megasynthase structural studies, and in vivo heterologous expression will continue to improve our fundamental understanding of polyketide synthesis as well as our ability to engineer the production of polyketides.

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Potential of Trichoderma spp. Secondary Metabolite in Controlling Vascular Streak Dieback (VSD) on Cacao Seedlings

Jurnal Tanaman Industri dan Penyegar ◽

10.21082/jtidp.v4n2.2017.p57-66 ◽

2017 ◽

Vol 4 (2) ◽

pp. 57 ◽

Cited By ~ 1

Author(s):

Rita Harni ◽

Widi Amaria ◽

Syafaruddin Syafaruddin ◽

Anis Herliyati Mahsunah

Keyword(s):

Secondary Metabolites ◽

Secondary Metabolite ◽

Plant Protection ◽

Plant Diseases ◽

Disease Suppression ◽

Bioactive Molecules ◽

Trichoderma Spp ◽

Randomized Design ◽

Dieback Disease ◽

Number Of Leaves

Trichoderma spp. is a beneficial microbe that produces bioactive molecules (secondary metabolites) containing antibiotics, enzymes, hormones, as well as toxins, which play an important role in plant diseases biocontrol. The research aimed to determine the potential of secondary metabolite Trichoderma spp. to control vascular streak dieback disease in cacao seedlings. The research was conducted in Plant Protection Laboratory of Indonesian Industrial and Beverage Crops Research Institute (IIBCRI) and farmers' garden in Balubus village, Lima Puluh Kota, West Sumatera, from April to August 2016. The research used a complete randomized design of 7 treatments using 5 replications, each treatment with 5 plants. The treatment was a secondary metabolite of T. virens LP1, T. hamatum LP2, T. amazonicum LP3, T. atroviride JB2, and T. viride PRD, control (no secondary metabolite), and chemical fungicide as comparison. The 3 months old cacao seedlings were treated with secondary metabolite of Trichoderma spp. by spraying the metabolite suspension throughout the leaf surface. The secondary metabolite applied once a week for 6 times. C. theobromae inoculation was conducted naturally by placing cacao seedlings under a cacao tree infected with VSD. Observations were incubation period, VSD intensity, and growth of cacao seedlings. The results showed that secondary metabolites potentially utilized for controlling VSD in cacao seedlings. The most potential secondary metabolites are T. amazonicum LP3 and T. virens LP1 with respective disease suppression up to 81.8% and 63.2% or higher than and equivalent chemical fungicide (63.6%), and can increase plants height, number of leaves, and girth diameter.

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