scholarly journals Structure of the Cell-Wall-Associated Polysaccharides from the Deep-Sea Marine Bacterium Devosia submarina KMM 9415T

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 665
Author(s):  
Maxim S. Kokoulin ◽  
Lyudmila A. Romanenko ◽  
Aleksandra S. Kuzmich ◽  
Oleg Chernikov
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Nmr Spectroscopy ◽  
Deep Sea ◽  
Marine Bacterium ◽  
Enzymatic Treatment ◽  
Chromatographic Techniques

Two cell-wall-associated polysaccharides were isolated and purified from the deep-sea marine bacterium Devosia submarina KMM 9415T, purified by ultracentrifugation and enzymatic treatment, separated by chromatographic techniques, and studied by sugar analyses and NMR spectroscopy. The first polysaccharide with a molecular weight of about 20.7 kDa was found to contain d-arabinose, and the following structure of its disaccharide repeating unit was established: →2)-α-d-Araf-(1→5)-α-d-Araf-(1→. The second polysaccharide was shown to consist of d-galactose and a rare component of bacterial glycans-d-xylulose: →3)-α-d-Galp-(1→3)-β-d-Xluf-(1→.

New Insights into Glycopeptide Antibiotic Binding to Cell Wall Precursors using SPR and NMR Spectroscopy

2014 ◽  
Vol 20 (24) ◽  
pp. 7363-7372 ◽  
Author(s):  
Juan Treviño ◽  
Carlos Bayón ◽  
Ana Ardá ◽  
Flavia Marinelli ◽  
Raffaella Gandolfi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Nmr Spectroscopy ◽  
Glycopeptide Antibiotic

Detection of cellulose in the cell wall of some red algae by 13C NMR spectroscopy

1994 ◽  
Vol 262 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Renato Toffanin ◽  
Svein H. Knutsen ◽  
Claudia Bertocchi ◽  
Roberto Rizzo ◽  
Erminio Murano
Keyword(s):  
Cell Wall ◽  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Red Algae ◽  
13C Nmr Spectroscopy

scholarly journals Neisseria gonorrhoeaePBP3 and PBP4 Facilitate NOD1 Agonist Peptidoglycan Fragment Release and Survival in Stationary Phase

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Ryan E. Schaub ◽  
Krizia M. Perez-Medina ◽  
Kathleen T. Hackett ◽  
Daniel L. Garcia ◽  
Joseph P. Dillard
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Inflammatory Response ◽  
Neisseria Gonorrhoeae ◽  
Wild Type ◽  
Cross Link ◽  
Content Type ◽  
Lytic Transglycosylases ◽  
Penicillin Binding Proteins ◽  
Proinflammatory Molecules

ABSTRACTNeisseria gonorrhoeaereleases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases or an amidase. However, >40% of the gonococcal cell wall is cross-linked, where the peptide stem on one peptidoglycan strand is linked to the peptide stem on a neighboring strand, suggesting that endopeptidases may be required for the release of many peptidoglycan fragments. Therefore, we characterized mutants with individual or combined mutations in genes for the low-molecular-mass penicillin-binding proteins PBP3 and PBP4. Mutations in eitherdacB, encoding PBP3, orpbpG, encoding PBP4, did not significantly reduce the release of peptidoglycan monomers or free peptides. A mutation indacBcaused the appearance of a larger-sized peptidoglycan monomer, the pentapeptide monomer, and an increased release of peptidoglycan dimers, suggesting the involvement of this enzyme in both the removal of C-terminald-Ala residues from stem peptides and the cleavage of cross-linked peptidoglycan. Mutation of bothdacBandpbpGeliminated the release of tripeptide-containing peptidoglycan fragments concomitantly with the appearance of pentapeptide and dipeptide peptidoglycan fragments and higher-molecular-weight peptidoglycan dimers. In accord with the loss of tripeptide peptidoglycan fragments, the level of human NOD1 activation by thedacB pbpGmutants was significantly lower than that by the wild type. We conclude that PBP3 and PBP4 overlap in function for cross-link cleavage and that these endopeptidases act in the normal release of peptidoglycan fragments during growth.

Tailored Enzymatic Treatment of chlorella Vulgaris Cell Wall Leads to Effective Disruption While Preserving Lipid Oxidative Stability

2021 ◽  
Author(s):  
Fabiola Dionisi ◽  
Greta Canelli ◽  
Isabelle Kuster ◽  
Billie Hauser ◽  
Patricia Murciano Martinez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Oxidative Stability ◽  
Chlorella Vulgaris ◽  
Enzymatic Treatment

scholarly journals Three new O-isocrotonyl-3-hydroxybutyric acid congeners produced by a sea anemone-derived marine bacterium of the genus Vibrio

2020 ◽  
Vol 16 ◽  
pp. 1869-1874
Author(s):  
Dandan Li ◽  
Enjuro Harunari ◽  
Tao Zhou ◽  
Naoya Oku ◽  
Yasuhiro Igarashi
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Marine Fish ◽  
Marine Bacterium ◽  
Sea Anemone ◽  
Pathogenic Bacterium ◽  
Hydroxybutyric Acid ◽  
Liquid Cultures ◽  
Growth Inhibitory ◽  
Biosynthetic Machinery

Liquid cultures of Vibrio sp. SI9, isolated from the outer tissue of the sea anemone Radianthus crispus, was found to produce three new O-isocrotonyl-3-hydroxybutyric acid derivatives, O-isocrotonyl-3-hydroxypentanoic acid (1), O-isocrotonyl-3-hydroxyhexanoic acid (2), and O-(Z)-2-hexenoyl-3-hydroxybutyric acid (3), together with the known O-isocrotonyl-3-hydroxybutyric acid (4). The structures of 1–3 were established by NMR spectroscopy and mass spectrometry, coupled with anisotropy-based chiral analysis, revealing the same R-configuration for all congeners 1–4. The compounds 1–4 were weakly growth-inhibitory against a marine fish ulcer pathogenic bacterium, Tenacibaculum maritimum NBRC16015. Structural similarities among 1–4, the O-isocrotonylated 3-hydroxybutyrate oligomers 5, and microbial biopolymer polyhydroxyalkanoates (PHA) suggest the presence of a common biosynthetic machinery, and hence a possible dehydrative modification at the hydroxy terminus of PHA.

scholarly journals Cell Wall Saturation Limit and Selected Properties of Thermally Modified Oak Wood and Cellulose

Forests ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 640 ◽  
Author(s):  
Richard Hrčka ◽  
Viera Kučerová ◽  
Tatiana Hýrošová ◽  
Vladimír Hönig
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Average Molecular Weight ◽  
Chemical Components ◽  
Water Molecules ◽  
Strong Negative Correlation ◽  
Oak Wood ◽  
Saturation Limit ◽  
Sorption Ability ◽  
Weighing Method

The interaction of water and oak wood is common in outdoor expositions and will remain a probable occurrence in the future. New insights into the recognition of a cell wall saturation limit are presented by a double-weighing method at 20 °C. The cell wall saturation limit, as the property of thermally modified oak wood, is significantly influenced by different treatment temperatures (20, 160, 180, 210 and 240 °C) on a 5% alpha level. A significantly higher equilibrium moisture content was reached by thermally modified oak wood at a temperature of 20 °C and relative humidity of 65% after its equilibrium in the water-in-reservoir. Moreover, the results are used in the treatment of woodchips to produce cellulose or decomposition of thermally modified wood to its basic chemical components. The investigated properties of cellulose revealed its relationship with water. The number of water molecules bonded to a cellulose chain was correlated with other measured compositions: average molecular weight, total crystalline index, lateral order index and polydispersity index. Analyses showed that there was a strong negative correlation between lateral order index and average molecular weight. The same was true between total crystalline index and average molecular weight. The rest of the properties were positively correlated with the number of water molecules bonded to glucopyranose. The results revealed the possible regeneration of a wood sorption ability after heat treatment and the stability of cellulose in such process.

The Porosity of Permeabilised Chara Cells

1989 ◽  
Vol 16 (3) ◽  
pp. 231 ◽  
Author(s):  
VA Shepherd ◽  
PB Goodwin
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Low Temperature ◽  
Fluorescent Probes ◽  
Maximum Diameter ◽  
Standard Procedures ◽  
Important Barrier

When charophyte cells are plasmolysed at low temperature in the presence of the chelator EGTA, they lose turgor and stop streaming. Since cyclosis can be restored with exogenous ATP, the cells are described as permeabilised. In this study, the permeabilised cell is described in terms of the measured permeability of plasmalemma and cell wall to a series of fluorescent probes of increasing molecular weight. Cells were permeabilised according to standard procedures, and cyclosis was restored with exogenous ATP. The plasmalemma was not disintegrated, but rather a limited increase in porosity occurred, permitting the passage of molecules up to 874 daltons in weight. This indicates that permeabilisation induces the formation of pores with a maximum diameter close to 3 nm. The cell wall was permeable to molecules up to 4158 Da, indicating a maximum porosity close to 4.8 nm. Thus, the plasmalemma remains an important barrier to diffusion in permeabilised cells.

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