scholarly journals An In Vitro Anti-Cancer Activity of Ocimum tenuiflorum Essential Oil by Inducing Apoptosis in Human Gastric Cancer Cell Line

Medicina ◽  
2021 ◽  
Vol 57 (8) ◽  
pp. 784
Author(s):  
Wongwarut Boonyanugomol ◽  
Kamolchanok Rukseree ◽  
Pornpan Prapatpong ◽  
Onrapak Reamtong ◽  
Seung-Chul Baik ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Migration And Invasion ◽  
Human Gastric Cancer ◽  
Ags Cells ◽  
Anti Cancer ◽  
Ocimum Tenuiflorum ◽  
Cancer Activity

The effects of Ocimum tenuiflorum essential oil (OTEO) against gastric cancer remain unknown and merit investigation. In the present study, the anti-cancer activity of OTEO was examined in a human gastric cancer cell line (AGS). After OTEO treatment, AGS cell viability was determined by an MTT assay, and inhibition of metastasis was determined by cell migration and invasion assays. The expression of apoptosis-related genes in treated AGS cells was determined by qRT-PCR. OTEO significantly decreased AGS cell viability in a dose-dependent manner (IC50 163.42 µg/mL) and effectively inhibited cell migration and invasion. Morphological examination demonstrated that OTEO induced cell shrinkage, chromatin condensation, and fragmentation, which are considered typical morphologies of apoptotic cell death. Pro-apoptotic genes (TP53, BAX, and BAK) were significantly up-regulated, while anti-apoptotic genes (BCL-2 and BCL-xL) were significantly down-regulated after treatment with OTEO. In addition, significantly increased gene expression was detected for CASP8, CASP9, and CASP3 in AGS cells exposed to OTEO. GC-MS analysis demonstrated that the major compound of OTEO was caryophyllene (25.85%) and α-pinene (11.66%). This in vitro study demonstrates for the first time that OTEO has potential anti-gastric cancer activity and may induce apoptosis in AGS cells through extrinsic and intrinsic pathways.

Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohammad Mostakhdem Hashemi ◽  
Majid Marjani ◽  
Nahid Poursharifi ◽  
Abdoljalal Marjani
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Incubation Time ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Root Extract ◽  
Human Gastric Cancer

Abstract One of the cancer-related deaths is gastric cancer in this area. Onosma dichroanthum Boiss. roots have been used as an antiseptic and anti-inflammatory for wound healing treatment. The aim of this study was to assess the in vitro cytotoxic and anticancer effects of O. dichroanthum Boiss. roots from the Golestan province of Iran. After identification of the taxonomical effect of O. dichroanthum Boiss., different concentrations of the hydroalcoholic root extract were used. Three different time periods (24, 48, and 72 h) were used to treat AGS gastric cancer and L-929 normal fibroblasts cell lines. The evaluation of different concentrations of root extract was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 48 h treatment affected cell survival, while the concentration of 64 μg/mL was determined as IC50 concentrations at 48 h incubation time. The 48 h incubation time with 64 μg/mL showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. Our results show that O. dichroanthum Boiss. roots extract may have cytotoxic and safe effects on gastric cancer cell-line and normal cells in 48 h treatment periods, respectively. The results indicated the O. dichroanthum Boiss. may be as an effective anticancer agent (gastric cancer).

scholarly journals Zeylenone Induces Mitochondrial Apoptosis and Inhibits Migration and Invasion in Gastric Cancer

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2149 ◽  
Author(s):  
Shuxian Yang ◽  
Yonghong Liao ◽  
Liyong Li ◽  
Xudong Xu ◽  
Li Cao
Keyword(s):  
Gastric Cancer ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Human Gastric Cancer ◽  
Mitochondrial Apoptosis ◽  
Apoptotic Pathway ◽  
Apoptotic Protein ◽  
Anti Cancer

The mortality of gastric cancer (GC) is increasing due to its high rates of recurrence and metastasis. Zeylenone (Zey), a type of naturally occurring cyclohexene oxide, was demonstrated to be effective in cancer patients. The aim of this study is to explore the anti-cancer effect of Zey against gastric cancer both in vitro and in vivo, as well as the underlying mechanisms. We found that Zey inhibited gastric tumor growth, as demonstrated by in vitro gastric cancer cell lines and in a human gastric cancer xenograft mouse model. Furthermore, Zey induced substantial apoptosis through a mitochondrial apoptotic pathway, involving mitochondrial transmembrane potential loss, caspase-3 activation, anti-apoptotic protein downregulation, and pro-apoptotic protein upregulation. Notably, we revealed for the first time that Zey suppressed invasion and migration by wound healing and transwell chamber assays. Through Western blotting, we further explored the potential mechanism of Zey’s anti-cancer activity. We found that Zey downregulated the expression of matrix metalloproteinase 2/9 (MMP 2/9) and inhibited the phosphorylation of AKT and ERK. In short, Zey, which induced mitochondrial apoptosis and inhibited proliferation, migration, and invasion, may be developed as a novel drug for the treatment of gastric cancer.

scholarly journals Effects of Huaier Polysaccharide SP1 on Gastric Cancer Cell Proliferation, Apoptosis, Migration, and Invasion by Regulating TGF-β/SMAD Signaling Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Miaoliang Chen ◽  
Ying Lu ◽  
Ruili Zhang ◽  
Tienan Bi ◽  
Shenkang Zhou
Keyword(s):  
Gastric Cancer ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Signal Pathway ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Gastric Cancer Cells

Objective. To study the effects of Huaier polysaccharide SP1 on the proliferation, apoptosis, migration, and invasion of gastric cancer cell line MGC-803 and the underlying mechanism. Methods. MGC-803 cells were cultured in vitro and treated with SP1. The effects of SP1 on the proliferation, apoptosis, migration, and invasion of MGC-803 cells were detected by CCK-8 assay, flow cytometry analysis, and Transwell assay, respectively. Western blot and qRT-PCR were used to detect the expression of related genes. Results. Our study showed that Huaier polysaccharide SP1 could inhibit proliferation, migration, invasion, and promote the apoptosis of MGC-803 cells in vitro in a dose-dependent manner. Huaier polysaccharide SP1 could inhibit the activation of TGF-β/SMAD signal pathway by upregulating SMAD7 expression, thereby downregulating the expression of SOX4, ZEB2, MMP9, Snail, and Slug. Conclusion. Huaier polysaccharide SP1 can regulate the proliferation, apoptosis, migration, and invasion of gastric cancer cells by promoting the expression of SMAD7 and inhibiting the activation of TGF-β/SMAD signal pathway as well as the expression of the downstream oncogenes.

Bombesin stimulates the in vitro growth of a human gastric cancer cell line

1994 ◽  
Vol 161 (3) ◽  
pp. 519-525 ◽  
Author(s):  
Richard J. Bold ◽  
Patrick S. Lowry ◽  
Jin Ishizuka ◽  
James F. Battey ◽  
Courtney M. Townsend ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer ◽  
In Vitro Growth ◽  
Human Gastric Cancer Cell

scholarly journals Curcumin Analog CH-5 Suppresses the Proliferation, Migration, and Invasion of the Human Gastric Cancer Cell Line HGC-27

Molecules ◽  
2018 ◽  
Vol 23 (2) ◽  
pp. 279 ◽  
Author(s):  
Gabriel Silva ◽  
Felipe Teixeira Lima ◽  
Viviane Seba ◽  
Ana Mendes Lourenço ◽  
Thaise Lucas ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Migration And Invasion ◽  
Human Gastric Cancer ◽  
Curcumin Analog ◽  
Human Gastric Cancer Cell
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