scholarly journals New Approach for the Construction and Calibration of Gas-Tight Setups for Biohydrogen Production at the Small Laboratory Scale

Metabolites ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 667
Author(s):  
Caroline Autenrieth ◽  
Shreya Shaw ◽  
Robin Ghosh
Keyword(s):  
Rhodospirillum Rubrum ◽  
Plastic Material ◽  
Photosynthetic Bacterium ◽  
Ideal Gas ◽  
Hydrogen Gas ◽  
Laboratory Scale ◽  
Biohydrogen Production ◽  
Modular System ◽  
Small Laboratory ◽  
Gas Tight

Biohydrogen production in small laboratory scale culture vessels is often difficult to perform and quantitate. One problem is that commonly used silicon tubing and improvised plastic connections used for constructing apparatus are cheap and easy to connect but are generally not robust for gases such as hydrogen. In addition, this type of apparatus presents significant safety concerns. Here, we demonstrate the construction of hydrogen-tight apparatus using a commercially available modular system, where plastic tubing and connections are made of explosion-proof dissipative plastic material. Using this system, we introduce a gas chromatograph calibration procedure, which can be easily performed without necessarily resorting to expensive commercial gas standards for the calibration of hydrogen gas concentrations. In this procedure, the amount of hydrogen produced by the reaction of sodium borohydride with water in a closed air-filled bottle is deduced from the observed decrease of the oxygen partial pressure, using the ideal gas law. Finally, the determined calibration coefficients and the gas-tight apparatus are used for the analysis of simultaneous oxygen consumption and hydrogen production of the purple photosynthetic bacterium, Rhodospirillum rubrum, during semi-aerobic growth in the dark.

scholarly journals Study of the Biodegrability of Degradable/Biodegradable Plastic Material in a Controlled Composting Environment

2012 ◽  
Vol 19 (3) ◽  
pp. 347-358 ◽  
Author(s):  
Magdalena Vaverková ◽  
František Toman ◽  
Dana Adamcová ◽  
Jana Kotovicová
Keyword(s):  
Plastic Material ◽  
Laboratory Scale ◽  
Biodegradable Plastic ◽  
Preliminary Evaluation ◽  
The Czech Republic ◽  
Chain Stores ◽  
Plastic Bags ◽  
Plastic Materials ◽  
Scale Test

Study of the Biodegrability of Degradable/Biodegradable Plastic Material in a Controlled Composting EnvironmentThe objective of this study was to determine the degrability/biodegradability of disposable plastic bags available on the market that are labeled as degradable/biodegradable and those certified as compost. The investigated materials were obtained from chain stores in the Czech Republic and Poland. Seven kinds of bags (commercially available) were used in this study. One of them was a disposable bag made of HDPE and mixed with totally degradable plastic additive (TDPA additive). Another was a disposable made of polyethylene with the addition of pro-oxidant additive (d2w additive). One was labeled as 100% degradable within various periods of time, from three months up to three years, and four were certified as compostable. The test was carried out in a controlled composting environment. The biodisintegration degree of the obtained pieces was evaluated following a modified version of ČSN EN 14806 Norm "Packaging - Preliminary evaluation of the disintegration of the packaging materials under simulated composting conditions in a laboratory scale test" and a modified version of ČSN EN ISO 20200 "Plastics - Determination of the degree of disintegration of plastic materials under simulated composting conditions in laboratory-scale test" (ISO 20200:2004). The emphasis was put on determination whether the bags are degradable/biodegradable or not.

scholarly journals Protein phosphorylation in the photosynthetic bacterium Rhodospirillum rubrum

FEBS Letters ◽  
1985 ◽  
Vol 181 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Loreto Holuigue ◽  
Héctor A. Lucero ◽  
Rubén H. Vallejos
Keyword(s):  
Protein Phosphorylation ◽  
Rhodospirillum Rubrum ◽  
Photosynthetic Bacterium

Prototype Cutterhead for Shaping-Lathe Headrigs Yields Pulp Chips and Cants

1983 ◽  
Vol 7 (3) ◽  
pp. 134-140
Author(s):  
I. Plough ◽  
P. Koch
Keyword(s):  
Size Distribution ◽  
Loblolly Pine ◽  
Laboratory Scale ◽  
Small Laboratory ◽  
Continuous Processes ◽  
Fiber Yield ◽  
Chip Size ◽  
Kraft Mill

Abstract Prior trials have demonstrated the feasibility of producing cants or rounded veneer bolts on shaping-lathe headrigs equipped with cutterheads that flake residues for structural flakeboard. This experiment demonstrated that shaping-lathe headrigs equipped with proper cutterheads will yield hardwood (oak) or softwood chips (1-inch long and 3/16-inch thick) suitable for pulping. Four-knife cutterheads running at 600 rpm, or three-knife cutterheads running at 800 rpm, will produce satisfactory pulp chips when scoring knives mounted ahead of chipping knives establish chip length. At a cutterhead speed of 600 rpm, negligible oversize chips and few fines were produced; lower cutterhead speeds yielded more oversize chips, and higher speeds more fines, but these differences in chip size distribution were small. Laboratory-scale pulping trials at a kraft mill indicated good fiber yield; the experimental loblolly pine chips cooked well in both batch and continuous processes, and were compatible with conventionally cut pulp chips.

scholarly journals First results of pipe pile static load test in small laboratory scale

2018 ◽  
Vol 251 ◽  
pp. 04038 ◽  
Author(s):  
Michal Baca ◽  
Jaroslaw Rybak
Keyword(s):  
Static Load ◽  
Axial Loading ◽  
Laboratory Scale ◽  
Load Test ◽  
Scale Model ◽  
Small Scale ◽  
Small Laboratory ◽  
Static Load Test ◽  
First Results ◽  
Load Tests

Presented laboratory testing program of tubular steel piles is a part of a bigger research program which contained static load tests in full scale and numerical simulations of conducted research. The main goal of the research is to compare static load tests with different working conditions of a shaft. The presented small scale model tests are the last part of the research. The paper contains the testing methodology description and first results of model pile axial loading. The static load tests in a small laboratory scale were conducted in a container filled with uniformly compacted medium sand (MSa). The first results of the investigation are presented in this paper, with the comparison of two pile capacities obtained for different roughness of the pile shaft (skin friction). The results are presented as load-displacement curves obtained by means of the Brinch-Hansen 80% method.

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