scholarly journals Three-Dimensional Modeling of Avascular Tumor Growth in Both Static and Dynamic Culture Platforms

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 580 ◽  
Author(s):  
Ali Taghibakhshi ◽  
Maryam Barisam ◽  
Mohammad Said Saidi ◽  
Navid Kashaninejad ◽  
Nam-Trung Nguyen
Keyword(s):  
Cell Culture ◽  
Tumor Growth ◽  
Growth Kinetics ◽  
Three Dimensional ◽  
Culture Systems ◽  
Tumor Growth Kinetics ◽  
Cell Culture Systems ◽  
Proposed Model ◽  
Microfluidic Cell Culture ◽  
Avascular Tumor

Microfluidic cell culture platforms are ideal candidates for modeling the native tumor microenvironment because they can precisely reconstruct in vivo cellular behavior. Moreover, mathematical modeling of tumor growth can pave the way toward description and prediction of growth pattern as well as improving cancer treatment. In this study, a modified mathematical model based on concentration distribution is applied to tumor growth in both conventional static culture and dynamic microfluidic cell culture systems. Apoptosis and necrosis mechanisms are considered as the main inhibitory factors in the model, while tumor growth rate and nutrient consumption rate are modified in both quiescent and proliferative zones. We show that such modification can better predict the experimental results of tumor growth reported in the literature. Using numerical simulations, the effects of the concentrations of the nutrients as well as the initial tumor radius on the tumor growth are investigated and discussed. Furthermore, tumor growth is simulated by taking into account the dynamic perfusion into the proposed model. Subsequently, tumor growth kinetics in a three-dimensional (3D) microfluidic device containing a U-shaped barrier is numerically studied. For this case, the effect of the flow rate of culture medium on tumor growth is investigated as well. Finally, to evaluate the impact of the trap geometry on the tumor growth, a comparison is made between the tumor growth kinetics in two frequently used traps in microfluidic cell culture systems, i.e., the U-shaped barrier and microwell structures. The proposed model can provide insight into better predicting the growth and development of avascular tumor in both static and dynamic cell culture platforms.

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

2011 ◽  
Vol 32 (5) ◽  
pp. 351-359 ◽  
Author(s):  
Andreas W. Daus ◽  
Michael Goldhammer ◽  
Paul G. Layer ◽  
Christiane Thielemann
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Dimensional Cell

Abstract A48: Quantifying stroma-tumor cell interactions in three-dimensional cell culture systems

2020 ◽  
Author(s):  
Marta Maria Cavo ◽  
Francesco Alemanno ◽  
Donatella Delle Cave ◽  
Eliana D'Amone ◽  
Adriano Barra ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tumor Cell ◽  
Three Dimensional ◽  
Cell Interactions ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Dimensional Cell

scholarly journals Three-Dimensional Cell Culture Systems in Radiopharmaceutical Cancer Research

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2765
Author(s):  
Alina Doctor ◽  
Verena Seifert ◽  
Martin Ullrich ◽  
Sandra Hauser ◽  
Jens Pietzsch
Keyword(s):  
Cell Culture ◽  
Cancer Research ◽  
Animal Experiments ◽  
Three Dimensional ◽  
3D Models ◽  
Ductal Adenocarcinoma ◽  
Multicellular Spheroids ◽  
Culture Systems ◽  
Advantages And Disadvantages ◽  
Cell Culture Systems

In preclinical cancer research, three-dimensional (3D) cell culture systems such as multicellular spheroids and organoids are becoming increasingly important. They provide valuable information before studies on animal models begin and, in some cases, are even suitable for reducing or replacing animal experiments. Furthermore, they recapitulate microtumors, metastases, and the tumor microenvironment much better than monolayer culture systems could. Three-dimensional models show higher structural complexity and diverse cell interactions while reflecting (patho)physiological phenomena such as oxygen and nutrient gradients in the course of their growth or development. These interactions and properties are of great importance for understanding the pathophysiological importance of stromal cells and the extracellular matrix for tumor progression, treatment response, or resistance mechanisms of solid tumors. Special emphasis is placed on co-cultivation with tumor-associated cells, which further increases the predictive value of 3D models, e.g., for drug development. The aim of this overview is to shed light on selected 3D models and their advantages and disadvantages, especially from the radiopharmacist’s point of view with focus on the suitability of 3D models for the radiopharmacological characterization of novel radiotracers and radiotherapeutics. Special attention is paid to pancreatic ductal adenocarcinoma (PDAC) as a predestined target for the development of new radionuclide-based theranostics.

scholarly journals Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices

Lab on a Chip ◽  
2014 ◽  
Vol 14 (11) ◽  
pp. 1816-1820 ◽  
Author(s):  
Björn Harink ◽  
Séverine Le Gac ◽  
David Barata ◽  
Clemens van Blitterswijk ◽  
Pamela Habibovic
Keyword(s):  
Cell Culture ◽  
Microfluidic Devices ◽  
Microtiter Plate ◽  
Physiological Control ◽  
Culture Systems ◽  
Physiological Conditions ◽  
Cell Culture Systems ◽  
Microfluidic Cell Culture

Microtiter plate-sized standalone chip holder for control of physiological conditions inside closed microfluidic cell culture systems, made from gas-impermeable materials.

Sign in / Sign up

Export Citation Format

Share Document