Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples

Droplet-based microfluidics is a versatile tool to reveal the dose–response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose–response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose–response data for 1D and 2D dose–response screenings are presented.

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Analgesic and anti-inflammatory dose–response relationship of 7.5 and 15mg meloxicam after lower third molar removal: a double-blind, randomized, crossover study

International Journal of Oral and Maxillofacial Surgery ◽

10.1016/j.ijom.2006.09.006 ◽

2007 ◽

Vol 36 (1) ◽

pp. 26-31 ◽

Cited By ~ 24

Author(s):

A.M. Calvo ◽

V.T. Sakai ◽

F.P.M. Giglio ◽

K.C.S. Modena ◽

B.L. Colombini ◽

...

Keyword(s):

Crossover Study ◽

Dose Response ◽

Third Molar ◽

Response Relationship ◽

Dose Response Relationship ◽

Double Blind ◽

Anti Inflammatory ◽

Relationship Of ◽

Randomized Crossover Study

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Underestimation of Dose - Response Relationship with Particular Reference to the Relationship between the Dietary Intake of Mercury and its Concentration in Blood

Human Toxicology ◽

10.1177/096032718800700204 ◽

1988 ◽

Vol 7 (2) ◽

pp. 129-132 ◽

Cited By ~ 16

Author(s):

J.C. Sherlock ◽

M.J. Quinn

Keyword(s):

Linear Regression ◽

Dietary Intake ◽

Dose Response ◽

Mercury Concentration ◽

Response Relationship ◽

Dose Response Relationship ◽

Blood Mercury ◽

Relationship Of ◽

The Relationship ◽

Blood Mercury Concentration

Wide discrepancies have been observed between controlled and uncontrolled intake studies of the relationship of blood mercury concentration to intake of mercury. The probable reason for the apparent discrepancies is that the within-subject variation of mercury intake in the uncontrolled studies was almost certainly considerably larger than the within-subject variation in blood mercury concentration; in these circumstances, the apparent slope obtained from a linear regression of blood mercury on intake will invariably be much smaller than the true slope. Studies of the exposure or intake of any substance should therefore include a consideration of the likely within-subject variation in the exposure or intake relative to that in the effect.

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A model analysis for dose–response relationship of warfarin in Japanese children: An introduction of the SIZE parameter

Drug Metabolism and Pharmacokinetics ◽

10.1016/j.dmpk.2016.03.007 ◽

2016 ◽

Vol 31 (3) ◽

pp. 234-241 ◽

Cited By ~ 2

Author(s):

Saki Nakamura ◽

Nao Watanabe ◽

Naoki Yoshimura ◽

Sayaka Ozawa ◽

Keiichi Hirono ◽

...

Keyword(s):

Dose Response ◽

Model Analysis ◽

Response Relationship ◽

Size Parameter ◽

Dose Response Relationship ◽

Japanese Children ◽

Relationship Of

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The Dose-Response Relationship of Ondansetron in the Prophylaxis of Pediatric Postoperative Emesis

Anesthesiology ◽

10.1097/00000542-199409001-01350 ◽

1994 ◽

Vol 81 (SUPPLEMENT) ◽

pp. A1351

Author(s):

M. F. Watcha ◽

P. J. Bras ◽

J. Pennant ◽

G. Cieslak ◽

D. Burnette

Keyword(s):

Dose Response ◽

Response Relationship ◽

Dose Response Relationship ◽

Relationship Of

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Dose–response relationship of mannitol and intracranial pressure: a metaanalysis

Yearbook of Critical Care Medicine ◽

10.1016/s0734-3299(08)79081-6 ◽

2009 ◽

Vol 2009 ◽

pp. 223-224

Author(s):

J.J. Provencio

Keyword(s):

Intracranial Pressure ◽

Dose Response ◽

Response Relationship ◽

Dose Response Relationship ◽

Relationship Of

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Investigations on the dose–response relationship of combined exposure to low doses of three anti-androgens in Wistar rats

Archives of Toxicology ◽

10.1007/s00204-017-2053-3 ◽

2017 ◽

Vol 91 (12) ◽

pp. 3961-3989 ◽

Cited By ~ 6

Author(s):

Steffen Schneider ◽

Karma C. Fussell ◽

Stephanie Melching-Kollmuss ◽

Roland Buesen ◽

Sibylle Gröters ◽

...

Keyword(s):

Dose Response ◽

Wistar Rats ◽

Low Doses ◽

Response Relationship ◽

Combined Exposure ◽

Dose Response Relationship ◽

Relationship Of

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Dose–response relationship of dicentric chromosomes in human lymphocytes obtained for the fission neutron therapy facility MEDAPP at the research reactor FRM II

Radiation and Environmental Biophysics ◽

10.1007/s00411-008-0202-z ◽

2008 ◽

Vol 48 (1) ◽

pp. 67-75 ◽

Cited By ~ 8

Author(s):

E. Schmid ◽

F. M. Wagner ◽

H. Romm ◽

L. Walsh ◽

H. Roos

Keyword(s):

Dose Response ◽

Research Reactor ◽

Human Lymphocytes ◽

Fission Neutron ◽

Response Relationship ◽

Dose Response Relationship ◽

Dicentric Chromosomes ◽

Neutron Therapy ◽

Relationship Of

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Comparative Effectiveness of Calabadion and Sugammadex to Reverse Non-depolarizing Neuromuscular-blocking Agents

Anesthesiology ◽

10.1097/aln.0000000000000868 ◽

2015 ◽

Vol 123 (6) ◽

pp. 1337-1349 ◽

Cited By ~ 45

Author(s):

Friederike Haerter ◽

Jeroen Cedric Peter Simons ◽

Urs Foerster ◽

Ingrid Moreno Duarte ◽

Daniel Diaz-Gil ◽

...

Keyword(s):

Dose Response ◽

Urine Analysis ◽

Ex Vivo ◽

Neuromuscular Blocking ◽

Response Relationship ◽

Binding Assays ◽

Dose Response Relationship ◽

Competition Binding ◽

Relationship Of

Abstract Background The authors evaluated the comparative effectiveness of calabadion 2 to reverse non-depolarizing neuromuscular-blocking agents (NMBAs) by binding and inactivation. Methods The dose–response relationship of drugs to reverse vecuronium-, rocuronium-, and cisatracurium-induced neuromuscular block (NMB) was evaluated in vitro (competition binding assays and urine analysis), ex vivo (n = 34; phrenic nerve hemidiaphragm preparation), and in vivo (n = 108; quadriceps femoris muscle of the rat). Cumulative dose–response curves of calabadions, neostigmine, or sugammadex were created ex vivo at a steady-state deep NMB. In living rats, the authors studied the dose–response relationship of the test drugs to reverse deep block under physiologic conditions, and they measured the amount of calabadion 2 excreted in the urine. Results In vitro experiments showed that calabadion 2 binds rocuronium with 89 times the affinity of sugammadex (Ka = 3.4 × 109 M−1 and Ka = 3.8 × 107 M−1). The results of urine analysis (proton nuclear magnetic resonance), competition binding assays, and ex vivo study obtained in the absence of metabolic deactivation are in accordance with an 1:1 binding ratio of sugammadex and calabadion 2 toward rocuronium. In living rats, calabadion 2 dose-dependently and rapidly reversed all NMBAs tested. The molar potency of calabadion 2 to reverse vecuronium and rocuronium was higher compared with that of sugammadex. Calabadion 2 was eliminated renally and did not affect blood pressure or heart rate. Conclusions Calabadion 2 reverses NMB induced by benzylisoquinolines and steroidal NMBAs in rats more effectively, i.e., faster than sugammadex. Calabadion 2 is eliminated in the urine and well tolerated in rats.

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