scholarly journals 4-Nitrophenol-Loaded Magnetic Mesoporous Silica Hybrid Materials for Spectrometric Aptasensing of Carcinoembryonic Antigen

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1138
Author(s):  
Jin Zhang ◽  
Dianping Tang
Keyword(s):  
Mesoporous Silica ◽  
Carcinoembryonic Antigen ◽  
Dynamic Range ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multiple Reactions ◽  
One Step ◽  
Hybridization Reaction ◽  
Magnetic Mesoporous Silica

Aptamer- or antibody-based sensing protocols have been reported for detecting carcinoembryonic antigen (CEA), but most exhibit complicated procedures or multiple reactions. In this work, we developed a one-step aptasensing protocol for the spectrometric determination of CEA based on 4-nitrophenol (4-NP)-loaded magnetic mesoporous silica nanohybrids (MMSNs) for bioresponsive controlled-release applications. To fabricate such a responsive–controlled sensing system, single-stranded complementary oligonucleotides relative to the CEA-specific aptamer were first modified on the aminated MMSN. Thereafter, 4-NP molecules blocked the pores with the assistance of the aptamers via a hybridization reaction. The introduced target CEA specifically reacted with the hybridized aptamer, thus detaching from the MMSN to open the gate. The loaded 4-NP molecules were released from the pores, as determined using ultraviolet–visible (UV–vis) absorption spectroscopy after magnetic separation. Under optimum conditions, the absorbance increased with an increase in the target CEA in the sample and exhibited a good linear relationship within the dynamic range of 0.1–100 ng mL−1, with a detection limit of 46 pg mL−1. Moreover, this system also displayed high specificity, good reproducibility, and acceptable accuracy for analyzing human serum specimens, in comparison with a commercialized human CEA-enzyme-linked immunosorbent assay (ELISA) kit.

Magnetic Mesoporous Silica/Chitosan/Polyproline: A Novel Nanocomposite Toward Sensing of Some Clinically Relevant Biomolecules

Nano LIFE ◽  
2017 ◽  
Vol 07 (03n04) ◽  
pp. 1750006 ◽  
Author(s):  
Mohammad Hasanzadeh ◽  
Soodabeh Hassanpour ◽  
Arezoo Saadati ◽  
Nasrin Shadjou ◽  
Ahad Mokhtarzadeh
Keyword(s):  
Mesoporous Silica ◽  
Surface Coverage ◽  
Linear Sweep Voltammetry ◽  
Differential Pulse ◽  
One Step ◽  
Pulse Voltammetry ◽  
Small Biomolecules ◽  
Magnetic Mesoporous Silica ◽  
Mcm 41

In this paper, free-radical polymerization inside magnetic mesoporous silica has been investigated in order to open a route to functional polymer–silica composite nanomaterials with well-defined mesoporosity. Proline monomers integrated with chitosan (CS) were electropolymerized into amino-functionalized magnetic mesoporous silica. The fabrication of polyproline-amino-functionalized magnetic mesoporous silica–CS nanohybrid on glassy carbon electrode (GCE) was performed using one step electrodeposition regime. Field emission scanning electron microscopy (FE-SEM) was confirmed as produced nanohybrid material containing polyproline (PPR) into the pores of magnetic (Fe3O[Formula: see text] mobile crystalline material-41 grafted with 3-aminopropyl groups (MMS) which leads to increase of surface coverage of PPR. The results indicate that PPR was successfully generated inside the pores of the amino-functionalized Mobil Composition of Matter No. 41 (nPrNH2-MCM-41) and that the amine group was capable of protonating the polymer, producing polyproline, the most conductive one, without the addition of another acid source during the polymerization step. Therefore, it was evaluated some electrochemical aspects of the prepared nanohybrid using cyclic voltammetry, differential pulse voltammetry and linear sweep voltammetry. Finally, the electroactivity of polyproline -Fe3O4-nPrNH2-MCM-41-chitosan nanohybrid (PPR-MMS-CS) modified GCE toward detection and determination of some clinically relevant small biomolecules was studied.

One‐step synthesis of amino‐functionalised magnetic mesoporous silica and synergistic adsorption kinetics of tetracycline and copper

2018 ◽  
Vol 13 (8) ◽  
pp. 1219-1223 ◽  
Author(s):  
Mancheng Zhang ◽  
Wei Wang ◽  
Zengyin Zhu ◽  
Changsheng Qu ◽  
Qing Zhou ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Adsorption Kinetics ◽  
One Step ◽  
Kinetics Of ◽  
Magnetic Mesoporous Silica

Development and Characterization of Anti-Estradiol Polyclonal Antibody

2012 ◽  
Vol 461 ◽  
pp. 67-70 ◽  
Author(s):  
Chao Ying Li ◽  
Jin Qing Jiang
Keyword(s):  
Polyclonal Antibody ◽  
Dynamic Range ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Uv Absorbance ◽  
Standard Curve ◽  
Ic50 Value ◽  
New Zealand White Rabbits

This paper reports an indirect competitive enzyme-linked immunosorbent assay (icELISA) using polyclonal antibody (pAb) for estradiol (E2) residues. After derivation, E2 haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) through 1-Ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method, and New Zealand white rabbits were immunized to produce anti-E2 pAb. The conjugation ratio of E2-BSA was proved to be 18.6:1 by an UV absorbance method. Based on the square matrix titration, an icELISA standard curve was developed. The dynamic range was from 0.16 to 128 ng/mL, with LOD and IC50 value of 0.08 ng/mL and 3.76 ng/mL, respectively. Except for a little cross-reactivity (16.2%) to estrone, this assay showed negligible cross-reactivity to other analogues tested. The results suggest that the produced anti-E2 pAb could be used to develop an icELISA method for the determination of E2 residues in animal-originally products.

scholarly journals Comparison of an Enzyme Immunoassay versus Mass Spectrometry-based Assay for the Quantitative Determination of the Procollagen Type I N-terminal Propeptide in Rat Serum

2009 ◽  
Vol 2 ◽  
pp. PRI.S3454 ◽  
Author(s):  
Monika Dzieciatkowska ◽  
Marci Copeland ◽  
Jinsam You ◽  
Jean-Pierre Wery ◽  
Mu Wang
Keyword(s):  
Mass Spectrometry ◽  
Enzyme Immunoassay ◽  
Dynamic Range ◽  
High Specificity ◽  
Specific Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Rat Serum ◽  
Procollagen Type ◽  
Procollagen Type I

Traditionally, antibody-based assays, such as enzyme immunoassay (EIA), enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA), are the primary tool for the targeted quantification of a specific protein. An antibody-based assay can be run at high-throughput and has extraordinary sensitivity and specificity. In the cases where antibody-based assays exist, the process of validating biomarker candidates can be relatively straightforward. However, the antibody-based approach is limited by the lack of availability of antibodies with high specificity. The development of a high quality antibody-based assays can be costly, time-consuming and a resource-intensive effort. Another disadvantage of antibody-based assays is that they often do not discriminate closely related isoforms. While the antibody development is central to the success of antibody-based platform, mass spectrometry (MS) provides alternative and complementary approach to existing antibody-based assays. The MS-based assays are becoming very popular for quantitative candidates proteins detection in a complex biological mixture. In the present paper, an in-house developed mass spectrometry (MS)-based assay was compared to a commercially available EIA in reproducibility, measurement accuracy, and dynamic range using rat procollagen type-I N-terminal propeptide (P1NP) as a model.

scholarly journals One-Step Preparation of Phenyl Boron-Modified Magnetic Mesoporous Silica for Selective Enrichment of cis-Diol-Containing Substances

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 603 ◽  
Author(s):  
Hua Fu ◽  
Jing Hu ◽  
Min Zhang ◽  
Yuerong Wang ◽  
Hongyang Zhang ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Selective Enrichment ◽  
One Step ◽  
Magnetic Mesoporous Silica

scholarly journals Flow Cytometric Microsphere-Based Immunoassay: Analysis of Secreted Cytokines in Whole-Blood Samples from Asthmatics

2001 ◽  
Vol 8 (4) ◽  
pp. 776-784 ◽  
Author(s):  
Christophe Camilla ◽  
Laurent Mély ◽  
Antoine Magnan ◽  
Brice Casano ◽  
Sabine Prato ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Whole Blood ◽  
Dynamic Range ◽  
Internal Standard ◽  
Multiplex Assay ◽  
Interleukin 4 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Flow Cytometric ◽  
Ifn Γ

ABSTRACT The ability of flow cytometry to resolve multiple parameters was used in a microsphere-based flow cytometric assay for the simultaneous determination of several cytokines in a sample. The flow cytometer microsphere-based assay (FMBA) for cytokines consists of reagents and dedicated software, specifically designed for the quantitative determination of cytokines. We have made several improvements in the multiplex assay: (i) dedicated software specific for the quantitative multiplex assay that processes data automatically, (ii) a stored master calibration curve with a two-point recalibration to adjust the stored curve periodically, and (iii) an internal standard to normalize the detection step in each sample. Overall analytical performance, including sensitivity, reproducibility, and dynamic range, was investigated for interleukin-4 (IL-4), IL-6, IL-10, IL-12, gamma interferon (IFN-γ), and tumor necrosis factor alpha. These assays were found to be reproducible and accurate, with a sensitivity in the picograms-per-milliliter range. Results obtained with FMBA correlate well with commercial enzyme-linked immunosorbent assay data (r > 0.98) for all cytokines assayed. This multiplex assay was applied to the determination of cytokine profiles in whole blood from atopic and nonatopic patients. Our results show that atopic subjects' blood produces more IL-4 (P = 0.003) and less IFN-γ (P = 0.04) than the blood of nonatopic subjects. However, atopic asthmatic subjects' blood produces significantly more IFN-γ than that of atopic nonasthmatic subjects (P = 0.03). The results obtained indicate that the FMBA technology constitutes a powerful system for the quantitative, simultaneous determination of secreted cytokines in immune diseases.

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