scholarly journals Bioactivity of Serratiochelin A, a Siderophore Isolated from a Co-Culture of Serratia sp. and Shewanella sp.

2020 ◽  
Vol 8 (7) ◽  
pp. 1042
Author(s):  
Yannik Schneider ◽  
Marte Jenssen ◽  
Johan Isaksson ◽  
Kine Østnes Hansen ◽  
Jeanette Hammer Andersen ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Biological Characterization ◽  
Marine Microbes ◽  
Mechanism Of Degradation ◽  
Iron Respiration ◽  
High Yields ◽  
The Given ◽  
And Staphylococcus Aureus

Siderophores are compounds with high affinity for ferric iron. Bacteria produce these compounds to acquire iron in iron-limiting conditions. Iron is one of the most abundant metals on earth, and its presence is necessary for many vital life processes. Bacteria from the genus Serratia contribute to the iron respiration in their environments, and previously several siderophores have been isolated from this genus. As part of our ongoing search for medicinally relevant compounds produced by marine microbes, a co-culture of a Shewanella sp. isolate and a Serratia sp. isolate, grown in iron-limited conditions, was investigated, and the rare siderophore serratiochelin A (1) was isolated with high yields. Compound 1 has previously been isolated exclusively from Serratia sp., and to our knowledge, there is no bioactivity data available for this siderophore to date. During the isolation process, we observed the degradation product serratiochelin C (2) after exposure to formic acid. Both 1 and 2 were verified by 1-D and 2-D NMR and high-resolution MS/MS. Here, we present the isolation of 1 from an iron-depleted co-culture of Shewanella sp. and Serratia sp., its proposed mechanism of degradation into 2, and the chemical and biological characterization of both compounds. The effects of 1 and 2 on eukaryotic and prokaryotic cells were evaluated, as well as their effect on biofilm formation by Staphylococcus epidermidis. While 2 did not show bioactivity in the given assays, 1 inhibited the growth of the eukaryotic cells and Staphylococcus aureus.

scholarly journals Discovery and Biological Characterization of the Auromomycin Chromophore as an Inhibitor of Biofilm Formation inVibrio cholerae

ChemBioChem ◽  
2013 ◽  
Vol 14 (16) ◽  
pp. 2209-2215 ◽  
Author(s):  
Kelly C. Peach ◽  
Andrew T. Cheng ◽  
Allen G. Oliver ◽  
Fitnat H. Yildiz ◽  
Roger G. Linington
Keyword(s):  
Biofilm Formation ◽  
Biological Characterization

scholarly journals Oral Administration of the Broad-Spectrum Antibiofilm Compound Toremifene Inhibits Candida albicans and Staphylococcus aureus Biofilm FormationIn Vivo

2014 ◽  
Vol 58 (12) ◽  
pp. 7606-7610 ◽  
Author(s):  
Kaat De Cremer ◽  
Nicolas Delattin ◽  
Katrijn De Brucker ◽  
Annelies Peeters ◽  
Soña Kucharíková ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Broad Spectrum ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Candida Krusei ◽  
Content Type ◽  
And Staphylococcus Aureus

ABSTRACTWe here report on thein vitroactivity of toremifene to inhibit biofilm formation of different fungal and bacterial pathogens, includingCandida albicans,Candida glabrata,Candida dubliniensis,Candida krusei,Pseudomonas aeruginosa,Staphylococcus aureus, andStaphylococcus epidermidis. We validated thein vivoefficacy of orally administered toremifene againstC. albicans and S. aureusbiofilm formation in a rat subcutaneous catheter model. Combined, our results demonstrate the potential of toremifene as a broad-spectrum oral antibiofilm compound.

scholarly journals New 3- hydrazonoindolin-2-one Cd(II) complexes with amino pyridine ligands, Synthesis, Characterization and biological activity evaluation

2020 ◽  
Vol 25 (2) ◽  
pp. 38
Author(s):  
Lubna W. Mohammed ◽  
Ahmed A. Irzoqi
Keyword(s):  
Staphylococcus Aureus ◽  
Biological Activity ◽  
Staphylococcus Epidermidis ◽  
Heterocyclic Ring ◽  
Azomethine Group ◽  
Gram Positive Bacteria ◽  
Pyridine Ligands ◽  
Tetrahedral Complexes ◽  
And Staphylococcus Aureus

This research includes synthesis and characterization of some of Cd(II) complexes with (3-hydrazonoindolin-2-one)(HZI) ligand and amino pyridine ligands.    Treatment equialmolar of CdCl2.2.5H2O and (HZI) ligand with two moles of n-aminopyridine (n-amp) (n:2,3,4) ligands afford a tetrahedral complexes of the type [Cd(HZI)(n-amp)2]Cl2, where (HZI) ligand behaves as a bidentate chelating fashion through the N atom of azomethine group and  O atom of carbonyl group. Whereas the (n-amp)(n: 2,3,4) was bonded monodentate mode through the N atom of heterocyclic ring. The prepared complexes have been characterized by molar conductivity, elemental analysis, infrared spectra and 1H-NMR and 13C-NMR spectra. Also the evaluation of biological activity of the prepared complexes against two types of gram positive bacteria (Staphylococcus Epidermidis and Staphylococcus aureus) and (Citrobacer Freundii) and gram negative, all prepared complexes showed activity against Staphylococcus aureus more than  amikacin, while the [Cd(HZI)(3-amp)2]Cl2 complex showed high activity against Staphylococcus Epidermidis and Citrobacter freundii more than another prepared complexes   http://dx.doi.org/10.25130/tjps.25.2020.028

scholarly journals Characterization of the growth dynamics and biofilm formation of Staphylococcus epidermidis strains isolated from contaminated platelet units

2014 ◽  
Vol 63 (6) ◽  
pp. 884-891 ◽  
Author(s):  
Hamza Ali ◽  
Valerie S. Greco-Stewart ◽  
Michael R. Jacobs ◽  
Roslyn A. Yomtovian ◽  
Ineke G. H. Rood ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Growth Dynamics ◽  
Extracellular Polysaccharide ◽  
Lag Phase ◽  
Infectious Risk ◽  
Slow Growing ◽  
Growth Experiments ◽  
Slow Grower

Bacterial contamination of platelet concentrates (PCs) poses the highest transfusion-associated infectious risk, with Staphylococcus epidermidis being a predominant contaminant. Herein, the growth dynamics of 20 S. epidermidis strains in PCs and regular media were characterized. Strains were categorized as fast (short lag phase) or slow (long lag phase) growers in PCs. All strains were evaluated for the presence of the biofilm-associated icaAD genes by PCR, their capability to produce extracellular polysaccharide (slime) on Congo red agar plates and their ability to form surface-attached aggregates (biofilms) in glucose-supplemented trypticase soy broth (TSBg) using a crystal violet staining assay. A subset of four strains (two slow growers and two fast growers) was further examined for the ability for biofilm formation in PCs. Two of these strains carried the icAD genes, formed slime and produced biofilms in TSBg and PCs, while the other two strains, which did not carry icaAD, did not produce slime or form biofilms in TSBg. Although the two ica-negative slime-negative strains did not form biofilms in media, they displayed a biofilm-positive phenotype in PCs. Although all four strains formed biofilms in PCs, the two slow growers formed significantly more biofilms than the fast growers. Furthermore, growth experiments of the two ica-positive strains in plasma-conditioned platelet bags containing TSBg revealed that a slow grower isolate was more likely to escape culture-based screening than a fast grower strain. Therefore, this study provides novel evidence that links S. epidermidis biofilm formation with slow growth in PCs and suggests that slow-growing biofilm-positive S. epidermidis would be more likely to be missed with automate culture.

scholarly journals The in vitro effect of xylitol on chronic rhinosinusitis biofilms

2016 ◽  
Vol 54 (4) ◽  
pp. 323-328
Author(s):  
R. Jain ◽  
T. Lee ◽  
T. Hardcastle ◽  
K. Biswas ◽  
F. Radcliff ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacteria Growth ◽  
Planktonic Bacteria ◽  
Crystal Violet Assay ◽  
Variable Activity ◽  
Vitro Effect ◽  
And Staphylococcus Aureus

Introduction: Biofilms have been implicated in chronic rhinosinusitis (CRS) and may explain the limited efficacy of antibiotics. There is a need to find more effective, non-antibiotic based therapies for CRS. This study examines the effects of xylitol on CRS biofilms and planktonic bacteria. Methods: Crystal violet assay and spectrophotometry were used to quantify the effects of xylitol (5% and 10% solutions) against Staphylococcus epidermidis, Pseudomonas aeruginosa, and Staphylococcus aureus. The disruption of established biofilms, inhibition of biofilm formation and effects on planktonic bacteria growth were investigated and compared to saline and no treatment. Results: Xylitol 5% and 10% significantly reduced biofilm biomass (S. epidermidis), inhibited biofilm formation (S. aureus and P. aeruginosa) and reduced growth of planktonic bacteria (S. epidermidis, S. aureus, and P. aeruginosa). Xylitol 5% inhibited formation of S. epidermidis biofilms more effectively than xylitol 10%. Xylitol 10% reduced S. epidermidis planktonic bacteria more effectively than xylitol 5%. Saline, xylitol 5% and 10% disrupted established biofilms of S. aureus when compared with no treatment. No solution was effective against established P. aeruginosa biofilm. Conclusions: Xylitol has variable activity against biofilms and planktonic bacteria in vitro and may have therapeutic efficacy in the management of CRS.

scholarly journals Identification of the sigB Operon inStaphylococcus epidermidis: Construction and Characterization of a sigB Deletion Mutant

2001 ◽  
Vol 69 (12) ◽  
pp. 7933-7936 ◽  
Author(s):  
Stefanie Kies ◽  
Michael Otto ◽  
Cuong Vuong ◽  
Friedrich Götz
Keyword(s):  
Staphylococcus Epidermidis ◽  
Sigma Factor ◽  
Biofilm Formation ◽  
Deletion Mutant ◽  
Alternative Sigma Factor ◽  
Global Regulator ◽  
Profound Influence ◽  
Cytoplasmic Proteins

ABSTRACT The role of the alternative sigma factor ςB inStaphylococcus epidermidis was investigated by the construction, complementation, and characterization of asigB deletion mutant. Electrophoretic analyses confirmed a profound influence of ςB on the expression of exoproteins and cytoplasmic proteins. Detailed investigation revealed reduced lipase and enhanced protease activity in the ςB mutant. Furthermore, no significant influence of ςB on heterologous biofilm formation or on the activity of the global regulator agr was detected.

scholarly journals Identification and Morphological Characterization of Biofilms Formed by Strains Causing Infection in Orthopedic Implants

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 649
Author(s):  
Débora C. Coraça-Huber ◽  
Lisa Kreidl ◽  
Stephan Steixner ◽  
Maximilian Hinz ◽  
Dietmar Dammerer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Epidermidis ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Orthopedic Implants ◽  
Orthopedic Implant ◽  
Colony Forming Units ◽  
Scanning Electron

Objectives: For a better understanding of the mechanisms involved in biofilm formation, we performed a broad identification and characterization of the strains affecting implants by evaluating the morphology of biofilms formed in vitro in correlation with tests of the strains’ antibiotic susceptibility in planktonic form. The ability of the strains to form biofilms in vitro was evaluated by means of colony forming units counting, metabolic activity tests of biofilm cells, and scanning electron microscopy. Methods: A total of 140 strains were isolated from patients with orthopedic implant-related infections during the period of 2015 to 2018. The identification of the isolates was carried out through microbiological cultures and confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility rates of the isolates were accessed according to EUCAST (European Committee on Antimicrobial Susceptibility Testing). The ability of all isolates to form biofilms in vitro was evaluated by counting the colony forming units, by measuring the metabolic activity of biofilm cells, and by analyzing the morphology of the formed biofilms using scanning electron microscopy. Results: From all the isolates, 41.84% (62 strains) were Staphylococcus epidermidis and 15.60% (22 strains) were Staphylococcus aureus. A significant difference in the capacity of biofilm formation was observed among the isolates. When correlating the biofilm forming capacity of the isolates to their antibiotic susceptibility rates, we observed that not all strains that were classified as resistant were biofilm producers in vitro. In other words, bacteria that are not good biofilm formers can show increased tolerance to multiple antibiotic substances. Conclusion: From 2015 until 2018, Staphylococcus epidermidis was the strain that caused most of the orthopedic implant-related infections in our hospital. Not all strains causing infection in orthopedic implants are able to form biofilms under in vitro conditions. Differences were observed in the number of cells and morphology of the biofilms. In addition, antibiotic resistance is not directly related to the capacity of the strains to form biofilms in vitro. Further studies should consider the use of in vitro culture conditions that better reproduce the joint environment and the growth of biofilms in humans.

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