scholarly journals Exploring the Chemical Space of Macro- and Micro-Algae Using Comparative Metabolomics

2021 ◽  
Vol 9 (2) ◽  
pp. 311
Author(s):  
Alison H. Hughes ◽  
Florent Magot ◽  
Ahmed F. Tawfike ◽  
Cecilia Rad-Menéndez ◽  
Naomi Thomas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Diversity ◽  
Chemical Space ◽  
Biological Activities ◽  
Chemical Diversity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Depth Analysis ◽  
Comparative Metabolomics ◽  
Chemical Profiles

With more than 156,000 described species, eukaryotic algae (both macro- and micro-algae) are a rich source of biological diversity, however their chemical diversity remains largely unexplored. Specialised metabolites with promising biological activities have been widely reported for seaweeds, and more recently extracts from microalgae have exhibited activity in anticancer, antimicrobial, and antioxidant screens. However, we are still missing critical information on the distinction of chemical profiles between macro- and microalgae, as well as the chemical space these metabolites cover. This study has used an untargeted comparative metabolomics approach to explore the chemical diversity of seven seaweeds and 36 microalgal strains. A total of 1390 liquid chromatography-mass spectrometry (LC-MS) features were detected, representing small organic algal metabolites, with no overlap between the seaweeds and microalgae. An in-depth analysis of four Dunaliella tertiolecta strains shows that environmental factors may play a larger role than phylogeny when classifying their metabolomic profiles.

scholarly journals Chemical Analysis of the Main Metabolites of Three Spontaneous Plants From the Saharan Area (Algeria)

2021 ◽  
Author(s):  
Imène RADJAI
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Analysis ◽  
Biological Activities ◽  
Time Of Flight ◽  
Chemical Study ◽  
Biological Functions ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Cotula Cinerea

Spontaneous plants metabolites are more widespread for their properties and biological functions. Also, natural products have reminded diverse scientists to take a delight in their medical and insecticidal applications linked to the environmental. A variety of metabolites have a defensive function for the plants. Thus, three spontaneous plants: Caroxylon imbricatum, Tetraena alba and Cotula cinerea collected from two ecotypes and analyzed by two known conventional methods:Gas Chromatography‐Mass Spectrometry GC QTOF(quadrupole time of flight )_MS and Liquid Chromatography-Mass spectrometry LCQTOF(quadrupole time of flight )_MS. The investigation conducted out on the identification and quantification of metabolites revealed the main metabolites which have biological activities as a part of an alternative to synthetic insecticides. The chemical study showed the presence of N-Butylbenzensulfonamide and Sulfoxycaprylicacid in the three plants. N-Carboxy-methionineresidue, Butanoicacid and Valine were found in those of Cotula cinerea and Caroxylon imbricatum (Forssk.). Artomunoxanthentrione, Glycoaldehyde, Indoline, ,Benzensulfonamide and Oxoproline were detected in extracts of Caroxylon imbricatum (Forssk.) and Tetraena alba (L.f.) In addition, Pyrroline is the only compound common in Cotula cinerea and Tetraena alba (L.f.).

Gut microbiota-mediated deglycosylation of ginsenoside Rb1 in rats: in vitro and in vivo insights from quantitative ultra-performance liquid chromatography-mass spectrometry analysis

2015 ◽  
Vol 7 (15) ◽  
pp. 6173-6181 ◽  
Author(s):  
Kang An ◽  
Zhang Shengjie ◽  
Shan Jinjun ◽  
Di Liuqing
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Ginsenoside Rb1 ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Ginsenoside Rb1, an ingredient of the herbal medicine Panax ginseng, possesses a variety of biological activities.

scholarly journals Liquid Chromatography Mass Spectrometry (LC-MS) Fingerprint Combined with Chemometrics for Identification of Metabolites Content and Biological Activities of Curcuma aeruginosa

2018 ◽  
Vol 18 (1) ◽  
pp. 43 ◽  
Author(s):  
Dewi Anggraini Septaningsih ◽  
Latifah Kosim Darusman ◽  
Farit Mochamad Afendi ◽  
Rudi Heryanto
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Partial Least Square ◽  
Least Square ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemometric Method

Curcuma aeruginosa is known as one of the components of herbal medicine with various biological activities. This research aims to identify the metabolites content of C. aeruginosa related to their biological activities using LC-MS fingerprint combined with chemometrics. C. aeruginosa from 3 areas in Java were collected and macerated with ethanol and then analyzed with LC-MS. Along with this analysis, the antioxidant activity of all samples was determined using CUPRAC method, and the toxicity was determined using Brine Shrimp Lethality Test (BSLT), and chemometric method was used Principle Component Analysis (PCA) and Partial Least square (PLS). Metabolites profiles showed 175 predicted compounds, in which the dominant compounds are from the sesquiterpene of Curcuma genus. The PCA metabolites profiles can separate the samples by their location of origin. Interpretation of the correlation between metabolites profiles and their bioactivities was determined using PLS technique. The results showed that the toxicity of samples was exerted by compounds with ion mass of 312.28 and 248.15, which have the highest antioxidant and toxicity potentials. Compounds with ion mass of 248.15 were predicted to be 9-Oxo-neoprocurcumenol, 7α,11α,-Epoxy-5β-hydroxy-9-guaiaen-8-one, Curcumenolactone A, or Curcumenolactone B. While compound with ion mass of 312.28 was predicted to tetrahydro-bisdemethoxycurcumin.

scholarly journals Digitizing mass spectrometry data to explore the chemical diversity and distribution of marine cyanobacteria and algae

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Tal Luzzatto-Knaan ◽  
Neha Garg ◽  
Mingxun Wang ◽  
Evgenia Glukhov ◽  
Yao Peng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Space ◽  
Biological Activities ◽  
Chemical Diversity ◽  
Mass Spectrometry Data ◽  
Marine Cyanobacteria ◽  
Dispersal Patterns ◽  
Screening Programs ◽  
Cyclic Lipopeptide ◽  
Cyanobacteria And Algae

Natural product screening programs have uncovered molecules from diverse natural sources with various biological activities and unique structures. However, much is yet underexplored and additional information is hidden in these exceptional collections. We applied untargeted mass spectrometry approaches to capture the chemical space and dispersal patterns of metabolites from an in-house library of marine cyanobacterial and algal collections. Remarkably, 86% of the metabolomics signals detected were not found in other available datasets of similar nature, supporting the hypothesis that marine cyanobacteria and algae possess distinctive metabolomes. The data were plotted onto a world map representing eight major sampling sites, and revealed potential geographic locations with high chemical diversity. We demonstrate the use of these inventories as a tool to explore the diversity and distribution of natural products. Finally, we utilized this tool to guide the isolation of a new cyclic lipopeptide, yuvalamide A, from a marine cyanobacterium.

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