Gut microbiota-mediated deglycosylation of ginsenoside Rb1 in rats: in vitro and in vivo insights from quantitative ultra-performance liquid chromatography-mass spectrometry analysis

2015 ◽  
Vol 7 (15) ◽  
pp. 6173-6181 ◽  
Author(s):  
Kang An ◽  
Zhang Shengjie ◽  
Shan Jinjun ◽  
Di Liuqing
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Ginsenoside Rb1 ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Ginsenoside Rb1, an ingredient of the herbal medicine Panax ginseng, possesses a variety of biological activities.

scholarly journals Serial coupling of chromatographic columns having orthogonal selectivity to improve the coverage of oxidised lipidome for mass spectrometry analysis

2019 ◽  
Author(s):  
Alpesh Thakker ◽  
Andrew Pitt ◽  
Corinne Spickett
Keyword(s):  
Mass Spectrometry ◽  
Chromatographic Separation ◽  
Biological Activities ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Hydrophilic Interaction ◽  
Spectrometry Analysis ◽  
Head Groups ◽  
Improved Methods

1AbstractPhospholipid oxidation (OxPL) generates a wide variety of products with potentially novel biological activities that may be associated with disease pathogenesis. To understand their role in disease requires precise information about their abundance in biological samples. Liquid chromatography-mass spectrometry (LCMS) is a sensitive technique that can provide detailed information about the oxidative lipidome, but challenges remain. Furthermore, variation in charge of the polar head groups and the extreme diversity of oxidised species make analysis of several classes of OxPLs within one analytical run challenging.The work in this study aims to develop improved methods for detection of OxPLs by improvement of chromatographic separation through the serial coupling of polystyrene-divinylbenzene based monolithic, and mixed-mode hydrophilic interaction (HILIC) with use of semi-targeted mass spectrometry approaches. The results suggests that by serially coupling two columns, HILIC and monolith, provided the better coverage of OxPL species in a single analytical run. We tested in-vitro generated oxidized species for phosphatidylcholine (PC) and phosphatidylethanolamine (PE) class and the combination of orthogonal chromatographic separation allowed separation of oxdised species from both the classes, which otherwise coeluted.

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