scholarly journals From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi

2021 ◽  
Vol 9 (9) ◽  
pp. 1924
Author(s):  
Pierre Joly ◽  
Alexandra Calteau ◽  
Aurélie Wauquier ◽  
Rémi Dumas ◽  
Mylène Beuvin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Yields ◽  
Antibiotic Resistance Genes ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
In Planta ◽  
Bacillus Velezensis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species Bacillus velezensis (synonym B. amyloliquefaciens subsp. plantarum or B. methylotrophicus), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other Bacillus spp. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent.

scholarly journals Penapisan isolat rizobakteri indigenos untuk pengendalian (Ganoderma boninense) di pre nursery kelapa sawit (Elaeis guineensis Jacq.)

Jurnal Agro ◽  
10.15575/4665 ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 110-122
Author(s):  
Yulmira Yanti ◽  
Imam Rifai ◽  
Yogie Aditya Pratama ◽  
Muhammad Ihsan Harahap
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Control Plant ◽  
Stem Rot ◽  
In Planta ◽  
Basal Stem Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
And Control

Rizobakteri merupakan kelompok bakteri yang aktif mengkolonisasi akar tanaman, meningkatkan pertumbuhan dan mengendalikan patogen tanaman. Penelitian ini bertujuan untuk memperoleh isolat rizobakteri indigenous  terbaik dalam meningkatkan pertumbuhan kelapa sawit dan mengendalikan penyakit busuk pangkal batang di pre-nursery kelapa sawit secara in planta serta karakterisasi kemampuan antagonisnya secara in vitro. Penelitian bersifat eksperimental terdiri atas 3 tahap dengan menggunakan Rancangan Acak Lengkap (RAL): (1) Isolasi dan karakterisasi isolat rizobakteri indigenous  di Kabupaten Pasaman Barat, (2) Pengujian isolat rizobakteri indigenous  (RBI) sebagai plant growth promoting rihzobacteria (PGPR), dan untuk pengendalian G.boninense di pre-nursery kelapa sawit terdiri dari 29 perlakuan (27 isolat RBI, tanpa inokulasi G. boninense sebagai kontrol positif, dan inokulasi G. boninense sebagai kontrol negatif) dengan masing-masing 5 ulangan, serta (3) Pengujian aktivitas antagonisme isolat RBI terhadap G. boninense. Data dianalisis dengan sidik ragam, apabila berbeda nyata dilanjutkan dengan uji Least Significance Different (LSD) pada taraf 5%. Hasil penelitian menunjukkan diperoleh tiga isolat terbaik (R10 2.2, R9 2.1, dan R10 2.3) yang mampu meningkatkan pertumbuhan kelapa sawit dan menekan perkembangan penyakit busuk pangkal batang G.boninense secara in planta dan in vitro.ABSTRACTRhizobacteria is a group of bacteria that actively colonize plant roots, increase growth and control plant pathogen. The objective of the research was to obtain indigenous rhizobacteria isolate (RBI) to increase growth and control basal stem rot on oil palm seedlings in in planta and characterize of antagonistic ability in in vitro. Experimental research consisted of 3 stages by using Completely Randomized Design (CRD): (1) Isolation of indigenous rhizobacteria in West Pasaman region, (2) Indigenous rhizobacteria isolate testing as a plant growth promoting rhizobacteria (PGPR) and to control of G. boninense on pre nursery of oil palm consisted of 29 treatments (27 RBI isolates, without G. boninense inoculation as positive control, and G. boninense inoculation as negative control) with 5 replications each. (3) Testing of RBI isolate antagonism activity towards G. boninense. Data were analyzed by variance, if the result significantly different, it was continued by using Least Significance Different (LSD) at 5% level. The results showed that best three isolates (R10 2.2, R9 2.1 and R10 2.3) were able to increase growth of palm oil and to suppress the development of G.boninense basal stem rot in in planta and in in vitro.

scholarly journals A Consortium of Rhizosphere-Competent Actinobacteria Exhibiting Multiple Plant Growth-Promoting Traits Improves the Growth of Avicennia marina in the United Arab Emirates

2021 ◽  
Vol 8 ◽  
Author(s):  
Khaled A. El-Tarabily ◽  
Arjun Sham ◽  
Alaa A. Elbadawi ◽  
Amira H. Hassan ◽  
Bashaer K. K. Alhosani ◽  
...  
Keyword(s):  
Plant Growth ◽  
United Arab Emirates ◽  
Maximum Velocity ◽  
Acc Deaminase ◽  
Avicennia Marina ◽  
Arid Ecosystems ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting

Gray mangrove (Avicennia marina) is the dominant vegetation distributed along the coast of the United Arab Emirates (UAE). Despite its performance as natural coastal guardians, very little is known about the reforestation projects to increase mangrove cover over the years in the UAE and in the Arabian Gulf. Plant growth-promoting actinobacteria (PGPA) were isolated from the mangrove rhizosphere sediments found in the UAE and were evaluated for their potential to produce plant growth regulators (PGRs) and to enhance mangrove growth under seawater irrigation conditions. In vitro screening identified nine rhizosphere-competent actinobacterial isolates, in a naturally competitive environment, of which Streptomyces coelicoflavus (Sc) showed a high phosphorus solubilizing activity. Moreover, Streptomyces polychromogenes (Sp), Streptomyces bacillaris (Sb), and Streptomyces ferrugineus (Sf) produced auxins, polyamines (PAs), and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively. Although sediment inoculation with single isolates significantly improved the dry biomass of mangrove shoots (43.2–74.0%) and roots (40.8–75.9%), the consortium of isolates (Sc/Sp/Sb/Sf) caused a greater increase in the dry weight of shoots (82.1%) and roots (81.6%) compared with seawater-irrigated plants (control). In our greenhouse experiments, the levels of photosynthetic pigments, in planta auxins, and PAs significantly increased in plant tissues inoculated with Sc/Sp/Sb/Sf; whereas ACC contents were reduced. This was also evident as the maximum velocity of rubisco carboxylation (Vcmax) increased four-fold in plants treated with the mixture of isolates over control. To the best of our knowledge, this is the first study reporting culturable halotolerant, rhizosphere-competent PGPA inhabiting salty and arid ecosystems applied individually or in combination to promote mangrove growth under harsh conditions such as those found in the Arabian coastal areas.

scholarly journals Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Lamia AOUAR ◽  
INAS BOUKELLOUL ◽  
ABDERRAHMANE BENADJILA
Keyword(s):  
Plant Growth ◽  
Phytopathogenic Fungi ◽  
Growth Promoter ◽  
Bioactive Substances ◽  
Plant Growth Promoting ◽  
Streptomyces Scabiei ◽  
Growth Promoting ◽  
Antagonistic Streptomyces

Abstract. Aouar L, Boukelloul I, Benadjila A. 2020. Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties. Biodiversitas 21: 5672-5683. To produce new bioactive substances of agricultural interest, extreme ecosystems can be a source of unexplored microorganisms. Accordingly, in this study, twenty-two actinobacteria strains were obtained from rhizospheric arid soils of palm groves collected from Biskra and El Oued in the Algerian Sahara. All isolates were examined for the in vitro antifungal potential towards phytopathogenic fungi: Aspergillus flavus, Verticillium dahlia, Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum as well as for their antibacterial property toward phytopathogenic bacteria: Streptomyces scabiei, Pectobacterium carotovorum and Agrobacterium tumefaciens. The three isolates (13%) that inhibited at least five pathogens were then selected, identified and assessed for their attributes to produce indole-3-acetic acid (IAA) and siderophores, to solubilize phosphate, and to antagonize Streptomyces scabiei in vivo. According to phylogenetic analysis performed with 16S rDNA sequence, chemotaxonomy and phenotypic characteristics, the strain SO1, which inhibited all tested pathogens, was assigned to Streptomyces flaveus. While, strains SO2 and SB1 were affiliated to Streptomyces enissocaesilis and Streptomyces albidoflavus, respectively. All strains produced IAA but only SO1 and SB1 were able to elaborate siderophores catecholate-type. Two strains SO1 and SO2 exhibited a capacity to solubilize phosphate and SO1 was able to suppress the pathogenic effect of Streptomyces scabiei on radish seedlings. The findings indicate that SO1 strain may reveal the potential for use as a biocontrol agent and plant growth promoter.

scholarly journals Selection and Assessment of Plant Growth-Promoting Rhizobacteria for Biological Control of Multiple Plant Diseases

2017 ◽  
Vol 107 (8) ◽  
pp. 928-936 ◽  
Author(s):  
Ke Liu ◽  
Molli Newman ◽  
John A. McInroy ◽  
Chia-Hui Hu ◽  
Joseph W. Kloepper
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Broad Spectrum ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Damping Off ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting

A study was designed to screen individual strains of plant growth-promoting rhizobacteria (PGPR) for broad-spectrum disease suppression in vitro and in planta. In a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. In a secondary screen, these 28 strains showed broad spectrum antagonistic activity to six different genera of pathogens, and 24 of the 28 strains produced five traits reported to be related to plant growth promotion, including nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore production, and biofilm formation. In advanced screens, the 28 PGPR strains selected in vitro were tested in planta for biological control of multiple plant diseases including bacterial spot of tomato caused by Xanthomonas axonopodis pv. vesicatoria, bacterial speck of tomato caused by Pseudomonas syringae pv. tomato, damping-off of pepper caused by Rhizoctonia solani, and damping-off of cucumber caused by Pythium ultimum. In all, 5 of the 28 tested strains significantly reduced three of the four tested diseases, and another 19 strains showed biological control to two tested diseases. To understand the observed broad-spectrum biocontrol capacity, antiSMASH was used to predict secondary metabolite clusters of selected strains. Multiple gene clusters encoding for secondary metabolites, e.g., bacillibactin, bacilysin, and microcin, were detected in each strain. In conclusion, selected individual PGPR strains showed broad-spectrum biocontrol activity to multiple plant diseases.

scholarly journals Fusarium Oxysporum f. sp. Cannabis Isolated from Cannabis Sativa L.: In Vitro and in Planta Biocontrol by a Plant Growth Promoting-Bacteria Consortium

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2436
Author(s):  
Marika Pellegrini ◽  
Claudia Ercole ◽  
Carmelo Gianchino ◽  
Matteo Bernardi ◽  
Loretta Pace ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fusarium Oxysporum ◽  
Cannabis Sativa ◽  
Antagonistic Activity ◽  
Plant Growth Promoting Bacteria ◽  
Dual Culture ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting

Industrial hemp (Cannabis sativa L.) is a multipurpose plant used in several fields. Several phytopathogens attack hemp crops. Fusarium oxysporum is a common fungal pathogen that causes wilt disease in nurseries and in field cultivation and causes high losses. In the present study, a pathogenic strain belonging to F. oxysporum f. sp. cannabis was isolated from a plant showing Fusarium wilt. After isolation, identification was conducted based on morphological and molecular characterizations and pathogenicity tests. Selected plant growth-promoting bacteria with interesting biocontrol properties—Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae and Burkholderia ambifaria—were tested against this pathogen. In vitro antagonistic activity was determined by the dual culture method. Effective strains (in vitro inhibition > of 50%) G. diazotrophicus, H. seropedicae and B. ambifaria were combined in a consortium and screened for in planta antagonistic activity in pre-emergence (before germination) and post-emergence (after germination). The consortium counteracted Fusarium infection both in pre-emergence and post-emergence. Our preliminary results show that the selected consortium could be further investigated as an effective biocontrol agent for the management of this pathogen.

scholarly journals Genomic Insights Into the Antifungal Activity and Plant Growth-Promoting Ability in Bacillus velezensis CMRP 4490

2021 ◽  
Vol 11 ◽  
Author(s):  
Gustavo Manoel Teixeira ◽  
Mirela Mosela ◽  
Maria Luiza Abreu Nicoletto ◽  
Renan Augusto Ribeiro ◽  
Mariangela Hungria ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Dna Sequences ◽  
Bacterial Genome ◽  
Coating Film ◽  
Bacillus Velezensis ◽  
Significant Difference ◽  
Plant Growth Promoting ◽  
Growth Promoting

The main objective of this study was to evaluate Bacillus velezensis strain CMRP 4490 regarding its ability to inhibit soil-borne plant pathogens and to increase plant growth. The study included evaluation of in vitro antifungal control, sequencing the bacterial genome, mining genes responsible for the synthesis of secondary metabolites, root colonization ability, and greenhouse studies for the assessment of plant growth–promoting ability. The strain was obtained from soil samples in the north of Paraná in Brazil and was classified as a B. velezensis, which is considered a promising biological control agent. In vitro assay showed that B. velezensis CMRP 4490 presented antagonistic activity against Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, and Rhizoctonia solani with a mycelial growth inhibition of approximately 60%, without any significant difference among them. To well understand this strain and to validate its effect on growth-promoting rhizobacteria, it was decided to explore its genetic content through genome sequencing, in vitro, and greenhouse studies. The genome of CMRP 4490 was estimated at 3,996,396 bp with a GC content of 46.4% and presents 4,042 coding DNA sequences. Biosynthetic gene clusters related to the synthesis of molecules with antifungal activity were found in the genome. Genes linked to the regulation/formation of biofilms, motility, and important properties for rhizospheric colonization were also found in the genome. Application of CMRP 4490 as a coating film on soybean increased from 55.5 to 64% on germination rates when compared to the control; no differences were observed among treatments for the maize germination. The results indicated that B. velezensis CMRP 4490 could be a potential biocontrol agent with plant growth–promoting ability.

scholarly journals Synthesis of Biogenic silver nanoparticles using plant growth-promoting bacteria: Potential use as biocontrol agent against phytopathogens

2021 ◽  
Vol 1 (1) ◽  
pp. 22-31
Author(s):  
Divya Mittal ◽  
Arun Kumar ◽  
Bhuvaneshwari Balasubramaniam ◽  
Rahul Thakur ◽  
Samarjeet Singh Siwal ◽  
...  
Keyword(s):  
Plant Growth ◽  
Burkholderia Cepacia ◽  
Phytopathogenic Fungi ◽  
Plant Growth Promoting Bacteria ◽  
Vis Spectroscopy ◽  
Silver Nps ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Potential Use

Biogenic nanoparticles (NPs) derived from microbes present an excellent opportunity to deal with various challenges in medicine, diagnosis, environment and agriculture. In the area of agriculture sciences, researchers are facing challenges related to excessive utilization of pesticides which can be answered by utilizing plant growth-promoting (PGP) microbes. Herein, we have employed the culture filtrate of two PBP bacteria strains, Serratia marcescens and Burkholderia cepacia to prepare biogenic silver NPs. The biogenic silver NPs were characterized by various techniques viz. UV-VIS spectroscopy, SEM, XRD and FTIR. The biogenic AgNPs were able to control the growth of phytopathogenic fungi Aspergillus niger, A. fumigatus, Fusarium oxysporum, Pythium sp., and Rosellinia sp. by more than 80% as examined by in vitro growth reduction on agar medium. Very significantly, the growth inhibition of seedlings by phytopathogenic fungi was efficiently rescued using biogenic AgNPs derived from PGP bacteria. These results indicate the potential use of biogenic NPs to reduce the burden of chemical-based pesticides.

scholarly journals Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria

2021 ◽  
pp. 66-72
Author(s):  
Alina Pastoshchuk ◽  
Yuliia Yumyna ◽  
Pavlyna Zelena ◽  
Larysa Skivka
Keyword(s):  
Plant Growth ◽  
Winter Wheat ◽  
Endophytic Bacteria ◽  
Wheat Variety ◽  
Wheat Grain ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experiments

scholarly journals Biocontrol of Soil-Borne Pathogens of Solanum lycopersicum L. and Daucus carota L. by Plant Growth-Promoting Actinomycetes: In Vitro and In Planta Antagonistic Activity

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1305
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Claudia Ercole ◽  
Beatrice Farda ◽  
Mahmoud Kitouni ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Pseudomonas Syringae ◽  
Daucus Carota ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
In Vitro Inhibition

Biotic stress caused by pathogenic microorganisms leads to damage in crops. Tomato and carrot are among the most important vegetables cultivated worldwide. These plants are attacked by several pathogens, affecting their growth and productivity. Fourteen plant growth-promoting actinomycetes (PGPA) were screened for their in vitro biocontrol activity against Solanum lycopersicum and Daucus carota microbial phytopathogens. Their antifungal activity was evaluated against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and Rhizoctonia solani (RHS). Antibacterial activity was evaluated against Pseudomonas syringae, Pseudomonas corrugata, Pseudomonas syringae pv. actinidiae, and Pectobacterium carotovorum subsp. carotovorum. Strains that showed good in vitro results were further investigated in vitro (cell-free supernatants activity, scanning electron microscope observations of fungal inhibition). The consortium of the most active PGPA was then utilized as biocontrol agents in planta experiments on S. lycopersicum and D. carota. The Streptomyces albidoflavus H12 and Nocardiopsis aegyptica H14 strains showed the best in vitro biocontrol activities. The diffusible and volatile compounds and cell-free supernatants of these strains showed both antifungal (in vitro inhibition up to 85%, hyphal desegregation and fungicidal properties) and antibacterial activity (in vitro inhibition >25 mm and bactericidal properties). Their consortium was also able to counteract the infection symptoms of microbial phytopathogens during in planta experiments, improving plant status. The results obtained highlight the efficacy of the selected actinomycetes strains as biocontrol agents of S. lycopersicum and D. carota.

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