Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis

Laccase is a widely used industrial oxidase for food processing, dye synthesis, paper making, and pollution remediation. At present, laccases used by industries come mainly from fungi. Plants contain numerous genes encoding laccase enzymes that show properties which are distinct from that of the fungal laccases. These plant-specific laccases may have better potential for industrial purposes. The aim of this work was to conduct a genome-wide search for the soybean laccase genes and analyze their characteristics and specific functions. A total of 93 putative laccase genes (GmLac) were identified from the soybean genome. All 93 GmLac enzymes contain three typical Cu-oxidase domains, and they were classified into five groups based on phylogenetic analysis. Although adjacent members on the tree showed highly similar exon/intron organization and motif composition, there were differences among the members within a class for both conserved and differentiated functions. Based on the expression patterns, some members of laccase were expressed in specific tissues/organs, while some exhibited a constitutive expression pattern. Analysis of the transcriptome revealed that some laccase genes might be involved in providing resistance to oomycetes. Analysis of the selective pressures acting on the laccase gene family in the process of soybean domestication revealed that 10 genes could have been under artificial selection during the domestication process. Four of these genes may have contributed to the transition of the soft and thin stem of wild soybean species into strong, thick, and erect stems of the cultivated soybean species. Our study provides a foundation for future functional studies of the soybean laccase gene family.

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Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Applied and Environmental Microbiology ◽

10.1128/aem.07880-11 ◽

2012 ◽

Vol 78 (11) ◽

pp. 4037-4045 ◽

Cited By ~ 54

Author(s):

Raúl Castanera ◽

Gúmer Pérez ◽

Alejandra Omarini ◽

Manuel Alfaro ◽

Antonio G. Pisabarro ◽

...

Keyword(s):

Gene Family ◽

Wheat Straw ◽

Pleurotus Ostreatus ◽

Submerged Fermentation ◽

Expression Profiles ◽

Water Soluble ◽

White Rot ◽

Accurate Estimation ◽

Laccase Gene ◽

Laccase Genes

ABSTRACTThe genome of the white rot basidiomycetePleurotus ostreatusincludes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) theLacc2andLacc10genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and theLaccgene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.

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The laccase gene family in Coprinopsis cinerea ( Coprinus cinereus )

Current Genetics ◽

10.1007/s00294-003-0452-x ◽

2004 ◽

Vol 45 (1) ◽

pp. 9-18 ◽

Cited By ~ 36

Author(s):

Patrik J. Hoegger ◽

Monica Navarro-Gonz�lez ◽

Sreedhar Kilaru ◽

Matthias Hoffmann ◽

Elisha D. Westbrook ◽

...

Keyword(s):

Gene Family ◽

Coprinus Cinereus ◽

Laccase Gene ◽

Coprinopsis Cinerea

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Mutant identification and characterization of the laccase gene family in Arabidopsis

Journal of Experimental Botany ◽

10.1093/jxb/erl022 ◽

2006 ◽

Vol 57 (11) ◽

pp. 2563-2569 ◽

Cited By ~ 95

Author(s):

Xiaoning Cai ◽

Elizabeth J. Davis ◽

Jenny Ballif ◽

Mingxiang Liang ◽

Emily Bushman ◽

...

Keyword(s):

Gene Family ◽

Laccase Gene ◽

Mutant Identification ◽

Identification And Characterization

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In Silico and Transcription Analysis of Trehalose-6-Phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence

Genes ◽

10.3390/genes12111652 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1652

Author(s):

Md Ashraful Islam ◽

Md Mustafizur Rahman ◽

Md Mizanor Rahman ◽

Xiujuan Jin ◽

Lili Sun ◽

...

Keyword(s):

Gene Family ◽

Stress Tolerance ◽

Leaf Senescence ◽

Brachypodium Distachyon ◽

Protein Structures ◽

Regulatory Elements ◽

Transcriptional Analysis ◽

Pcr Analysis ◽

Evolutionary Status ◽

Transcription Analysis

Trehalose-6-phosphate phosphatase (TPP) genes take part in trehalose metabolism and also in stress tolerance, which has been well documented in many species but poorly understood in wheat. The present research has identified a family of 31 TPP genes in Triticum aestivum L. through homology searches and classified them into five clades by phylogenetic tree analysis, providing evidence of an evolutionary status with Hordeum vulgare, Brachypodium distachyon and Oryza sativa. The exon-intron distribution revealed a discrete evolutionary history and projected possible gene duplication occurrences. Furthermore, different computational approaches were used to analyze the physical and chemical properties, conserved domains and motifs, subcellular and chromosomal localization, and three-dimensional (3-D) protein structures. Cis-regulatory elements (CREs) analysis predicted that TaTPP promoters consist of CREs related to plant growth and development, hormones, and stress. Transcriptional analysis revealed that the transcription levels of TaTPPs were variable in different developmental stages and organs. In addition, qRT-PCR analysis showed that different TaTPPs were induced under salt and drought stresses and during leaf senescence. Therefore, the findings of the present study give fundamental genomic information and possible biological functions of the TaTPP gene family in wheat and will provide the path for a better understanding of TaTPPs involvement in wheat developmental processes, stress tolerance, and leaf senescence.

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Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Genes ◽

10.3390/genes10121045 ◽

2019 ◽

Vol 10 (12) ◽

pp. 1045

Author(s):

Lianlian Yan ◽

Ruiping Xu ◽

Yinbing Bian ◽

Hongxian Li ◽

Yan Zhou

Keyword(s):

Gene Family ◽

Fruiting Body ◽

Lentinula Edodes ◽

Expression Profiles ◽

Draft Genome ◽

White Rot ◽

Laccase Gene ◽

Fruiting Body Formation ◽

Body Formation ◽

Laccase Genes

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

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