scholarly journals Unveiling the Molecular Basis of Mascarpone Cheese Aroma: VOCs analysis by SPME-GC/MS and PTR-ToF-MS

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1242 ◽  
Author(s):  
Vittorio Capozzi ◽  
Valentina Lonzarich ◽  
Iuliia Khomenko ◽  
Luca Cappellin ◽  
Luciano Navarini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Solid Phase ◽  
Dairy Product ◽  
Proton Transfer Reaction ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Analyzer ◽  
Commercial Sample ◽  
Cheese Aroma ◽  
The One ◽  
Tof Ms

Mascarpone, a soft-spread cheese, is an unripened dairy product manufactured by the thermal-acidic coagulation of milk cream. Due to the mild flavor and creamy consistency, it is a base ingredient in industrial, culinary, and homemade preparations (e.g., it is a key constituent of a widely appreciated Italian dessert ‘Tiramisù’). Probably due to this relevance as an ingredient rather than as directly consumed foodstuff, mascarpone has not been often the subject of detailed studies. To the best of our knowledge, no investigation has been carried out on the volatile compounds contributing to the mascarpone cheese aroma profile. In this study, we analyzed the Volatile Organic Compounds (VOCs) in the headspace of different commercial mascarpone cheeses by two different techniques: Headspace-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (HS-SPME GC-MS) and Proton-Transfer Reaction-Mass Spectrometry coupled to a Time of Flight mass analyzer (PTR-ToF-MS). We coupled these two approaches due to the complementarity of the analytical potential—efficient separation and identification of the analytes on the one side (HS-SPME GC-MS), and effective, fast quantitative analysis without any sample preparation on the other (PTR-ToF-MS). A total of 27 VOCs belonging to different chemical classes (9 ketones, 5 alcohols, 4 organic acids, 3 hydrocarbons, 2 furans, 1 ester, 1 lactone, 1 aldehyde, and 1 oxime) have been identified by HS-SPME GC-MS, while PTR-ToF-MS allowed a rapid snapshot of volatile diversity confirming the aptitude to rapid noninvasive quality control and the potential in commercial sample differentiation. Ketones (2-heptanone and 2-pentanone, in particular) are the most abundant compounds in mascarpone headspace, followed by 2-propanone, 2-nonanone, 2-butanone, 1-pentanol, 2-ethyl-1-hexanol, furfural and 2-furanmethanol. The study also provides preliminary information on the differentiation of the aroma of different brands and product types.

scholarly journals Effects of modular ion-funnel technology onto analysis of breath VOCs by means of real-time mass spectrometry

2020 ◽  
Vol 412 (26) ◽  
pp. 7131-7140
Author(s):  
Giovanni Pugliese ◽  
Felix Piel ◽  
Phillip Trefz ◽  
Philipp Sulzer ◽  
Jochen K. Schubert ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Human Subjects ◽  
Proton Transfer Reaction ◽  
Mass Analyzer ◽  
Real Time Monitoring ◽  
Healthy Human ◽  
Flight Mass Spectrometry ◽  
Ion Funnel ◽  
Tof Ms

Abstract Proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) is a powerful tool for real-time monitoring of trace concentrations of volatile organic compounds (VOCs). The sensitivity of PTR-ToF-MS also depends on the ability to effectively focus and transmit ions from the relatively high-pressure drift tube (DT) to the low-pressure mass analyzer. In the present study, a modular ion-funnel (IF) is placed adjacent to the DT of a PTR-ToF-MS instrument to improve the ion-focusing. IF consists of a series of electrodes with gradually decreasing orifice diameters. Radio frequency (RF) voltage and direct current (DC) electric field are then applied to the electrodes to get the ions focused. We investigated the effect of the RF voltage and DC field on the sensitivity of a pattern of VOCs including hydrocarbons, alcohols, aldehydes, ketones, and aromatic compounds. In a proof-of-concept study, the instrument operating both as normal DT (DC-mode) and at optimal IF conditions (RF-mode) was applied for the breath analysis of 21 healthy human subjects. For the range of investigated VOCs, an improvement of one order of magnitude in sensitivity was observed in RF-mode compared with DC-mode. Limits of detection could be improved by a factor of 2–4 in RF-mode compared with DC-mode. Operating the instrument in RF-mode allowed the detection of more compounds in the exhaled air compared with DC-mode. Incorporation of the IF considerably improved the performance of PTR-ToF-MS allowing the real-time monitoring of a larger number of potential breath biomarkers.

Isolation and quantification of cholesterol oxides in dairy products by selected ion monitoring mass spectrometry

1995 ◽  
Vol 62 (1) ◽  
pp. 101-113 ◽  
Author(s):  
Jacob H. Nielsen ◽  
Carl Erik Olsen ◽  
Christina Duedahl ◽  
Leif H. Skibsted
Keyword(s):  
Mass Spectrometry ◽  
Dairy Products ◽  
Solid Phase ◽  
Dairy Product ◽  
Oxidation Products ◽  
Gas Chromatography Mass Spectrometry ◽  
Cholesterol Oxidation ◽  
Preparative Hplc ◽  
Selected Ion Monitoring ◽  
Limit Of Quantification

SUMMARYA method for isolation, detection and quantification of cholesterol oxidation products based on solid phase extraction in combination with preparative HPLC and gas chromatography–mass spectrometry selected ion monitoring has been developed for dairy products. The isolation procedure had a high recovery and artifact formation was minimal, as shown by isotope labelling. The limits of detection ranged from 0.3 to 35 pg/μl of the isomeric forms of 7-hydroxycholesterol, 20α-hydroxycholesterol, the isomeric forms of cholesterol-5,6-epoxides, cholestanetriol, 25-hydroxycholesterol and 7-ketocholesterol corresponding to a limit of quantification of 2–6 ng oxysterol/g lipid in the dairy product, depending on the nature of the cholesterol oxidation product.

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