scholarly journals Research Progress of the Biosynthesis of Natural Bio-Antibacterial Agent Pulcherriminic Acid in Bacillus

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5611
Author(s):  
Siqi Yuan ◽  
Xihao Yong ◽  
Ting Zhao ◽  
Yuan Li ◽  
Jun Liu
Keyword(s):  
Gene Regulation ◽  
Antibacterial Agent ◽  
Biosynthetic Pathway ◽  
Enzymatic Catalysis ◽  
Fermented Food ◽  
Combinatorial Biosynthesis ◽  
Research Progress ◽  
Iron Ions ◽  
Cyclic Dipeptide ◽  
Catalytic Mechanisms

Pulcherriminic acid is a cyclic dipeptide found mainly in Bacillus and yeast. Due to the ability of pulcherriminic acid to chelate Fe3+ to produce reddish brown pulcherrimin, microorganisms capable of synthesizing pulcherriminic acid compete with other microorganisms for environmental iron ions to achieve bacteriostatic effects. Therefore, studying the biosynthetic pathway and their enzymatic catalysis, gene regulation in the process of synthesis of pulcherriminic acid in Bacillus can facilitate the industrial production, and promote the wide application in food, agriculture and medicine industries. After initially discussing, this review summarizes current research on the synthesis of pulcherriminic acid by Bacillus, which includes the crystallization of key enzymes, molecular catalytic mechanisms, regulation of synthetic pathways, and methods to improve efficiency in synthesizing pulcherriminic acid and its precursors. Finally, possible applications of pulcherriminic acid in the fermented food, such as Chinese Baijiu, applying combinatorial biosynthesis will be summarized.

scholarly journals (6S)-6-fluoroshikimic acid, an antibacterial agent acting on the aromatic biosynthetic pathway.

1994 ◽  
Vol 38 (2) ◽  
pp. 403-406 ◽  
Author(s):  
G M Davies ◽  
K J Barrett-Bee ◽  
D A Jude ◽  
M Lehan ◽  
W W Nichols ◽  
...  
Keyword(s):  
Antibacterial Agent ◽  
Biosynthetic Pathway

scholarly journals Genome mining of novel rubiginones from Streptomyces sp. CB02414 and characterization of the post-PKS modification steps in rubiginone biosynthesis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jingyan Zhang ◽  
Ying Sun ◽  
Yeji Wang ◽  
Xin Chen ◽  
Lu Xue ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Biosynthetic Pathway ◽  
Scale Up ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Wild Type ◽  
Aromatic Polyketides ◽  
A Genome

Abstract Background Rubiginones belong to the angucycline family of aromatic polyketides, and they have been shown to potentiate the vincristine (VCR)-induced cytotoxicity against VCR-resistant cancer cell lines. However, the biosynthetic gene clusters (BGCs) and biosynthetic pathways for rubiginones have not been reported yet. Results In this study, based on bioinformatics analysis of the genome of Streptomyces sp. CB02414, we predicted the functions of the two type II polyketide synthases (PKSs) BGCs. The rub gene cluster was predicted to encode metabolites of the angucycline family. Scale-up fermentation of the CB02414 wild-type strain led to the discovery of eight rubiginones, including five new ones (rubiginones J, K, L, M, and N). Rubiginone J was proposed to be the final product of the rub gene cluster, which features extensive oxidation on the A-ring of the angucycline skeleton. Based on the production profiles of the CB02414 wild-type and the mutant strains, we proposed a biosynthetic pathway for the rubiginones in CB02414. Conclusions A genome mining strategy enabled the efficient discovery of new rubiginones from Streptomyces sp. CB02414. Based on the isolated biosynthetic intermediates, a plausible biosynthetic pathway for the rubiginones was proposed. Our research lays the foundation for further studies on the mechanism of the cytochrome P450-catalyzed oxidation of angucyclines and for the generation of novel angucyclines using combinatorial biosynthesis strategies.

scholarly journals Biosynthetic engineering of the antifungal, anti-MRSA auroramycin

2019 ◽  
Author(s):  
Wan Lin Yeo ◽  
Elena Heng ◽  
Lee Ling Tan ◽  
Yi Wee Lim ◽  
Kuan Chieh Ching ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Genome Editing ◽  
Gene Cluster ◽  
Biosynthetic Pathway ◽  
Biosynthetic Gene Cluster ◽  
Combinatorial Biosynthesis ◽  
Biosynthetic Gene ◽  
Antifungal Activities ◽  
Biosynthetic Engineering

AbstractUsing an established CRISPR-Cas mediated genome editing technique for streptomycetes, we explored the combinatorial biosynthesis potential of the auroramycin biosynthetic gene cluster in Streptomyces roseoporous. Auroramycin is a potent anti-MRSA polyene macrolactam. In addition, it also displays antifungal activities, which is unique among structurally similar polyene macrolactams, such as incednine and silvalactam. In this work, we employed different engineering strategies to target glycosylation and acylation biosynthetic machineries within its recently elucidated biosynthetic pathway. Six auroramycin analogs with variations in C-, N-methylation, hydroxylation and extender units incorporation were produced and characterized. By comparing the bioactivity profiles of these analogs, we determined that unique disaccharide motif of auroramycin is essential for its antimicrobial bioactivity. We further demonstrated that C-methylation of the 3, 5-epi-lemonose unit, which is unique among structurally similar polyene macrolactams, is key to its antifungal activity.

The evolutionary acquisition and mode of functions of promoter-associated non-coding RNAs (pancRNAs) for mammalian development

2021 ◽  
Author(s):  
Boyang An ◽  
Tomonori Kameda ◽  
Takuya Imamura
Keyword(s):  
Gene Regulation ◽  
Neural Differentiation ◽  
Single Copy ◽  
Research Progress ◽  
Mammalian Development ◽  
Comparative Epigenomics ◽  
Specific Manner ◽  
A Cell ◽  
Non Coding Rnas ◽  
Mammalian Embryonic Development

Abstract Increasing evidence has shown that many long non-coding RNAs (lncRNAs) are involved in gene regulation in a variety of ways such as transcriptional, post-transcriptional and epigenetic regulation. Promoter-associated non-coding RNAs (pancRNAs), which are categorized into the most abundant single-copy lncRNA biotype, play vital regulatory roles in finely tuning cellular specification at the epigenomic level. In short, pancRNAs can directly or indirectly regulate downstream genes to participate in the development of organisms in a cell-specific manner. In this review, we will introduce the evolutionarily acquired characteristics of pancRNAs as determined by comparative epigenomics and elaborate on the research progress on pancRNA-involving processes in mammalian embryonic development, including neural differentiation.

Recent Advances in Study of Ginsenoside Biosynthetic Pathway in Panax ginseng

2013 ◽  
Vol 773 ◽  
pp. 368-373 ◽  
Author(s):  
Shi Kun Jin ◽  
Shou Jing Zhao
Keyword(s):  
Panax Ginseng ◽  
Biosynthetic Pathway ◽  
Metabolic Flux ◽  
Economic Benefits ◽  
Research Progress ◽  
Human Being ◽  
Methylerythritol Phosphate ◽  
Genetic Level ◽  
Bioactive Ingredients ◽  
Increasing Demand

Ginsenosides, the major bioactive ingredients of P. ginseng can improve the anti-disease abilities of human being, and generate significant social and economic benefits. However, along with gradually or rapidly or dramatically increasing demand of the ginsenosides, extensive studies have focused on regulating the ginsenoside biosynthetic pathway on a genetic level. This review provides the latest research progress on biosynthetic pathway of ginsenosides, including the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathway, which is newly discovered and located in P. ginseng. Moreover, it also indicated lanosterol synthase metabolic flux present in P. ginseng.

Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum

Planta ◽  
2010 ◽  
Vol 233 (2) ◽  
pp. 343-355 ◽  
Author(s):  
Young Soon Kim ◽  
Jung Hyun Cho ◽  
Sangkyu Park ◽  
Jung-Yeon Han ◽  
Kyoungwhan Back ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Methyl Jasmonate ◽  
Biosynthetic Pathway ◽  
Squalene Synthase ◽  
Bupleurum Falcatum

scholarly journals Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by Taxus Acyltransferases

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2855
Author(s):  
Tao Wang ◽  
Lingyu Li ◽  
Weibing Zhuang ◽  
Fengjiao Zhang ◽  
Xiaochun Shu ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Structural Diversity ◽  
Underlying Mechanism ◽  
Research Progress ◽  
Enzymatic Reactions ◽  
Acylation Reaction ◽  
Taxol Biosynthesis ◽  
The Core ◽  
Coa Ligase ◽  
Acylation Reactions

Taxol is one of the most effective anticancer drugs in the world that is widely used in the treatments of breast, lung and ovarian cancer. The elucidation of the taxol biosynthetic pathway is the key to solve the problem of taxol supply. So far, the taxol biosynthetic pathway has been reported to require an estimated 20 steps of enzymatic reactions, and sixteen enzymes involved in the taxol pathway have been well characterized, including a novel taxane-10β-hydroxylase (T10βOH) and a newly putative β-phenylalanyl-CoA ligase (PCL). Moreover, the source and formation of the taxane core and the details of the downstream synthetic pathway have been basically depicted, while the modification of the core taxane skeleton has not been fully reported, mainly concerning the developments from diol intermediates to 2-debenzoyltaxane. The acylation reaction mediated by specialized Taxus BAHD family acyltransferases (ACTs) is recognized as one of the most important steps in the modification of core taxane skeleton that contribute to the increase of taxol yield. Recently, the influence of acylation on the functional and structural diversity of taxanes has also been continuously revealed. This review summarizes the latest research advances of the taxol biosynthetic pathway and systematically discusses the acylation reactions supported by Taxus ACTs. The underlying mechanism could improve the understanding of taxol biosynthesis, and provide a theoretical basis for the mass production of taxol.

Sign in / Sign up

Export Citation Format

Share Document