scholarly journals Ratiometric Fluorescence Assay for Nitroreductase Activity: Locked-Flavylium Fluorophore as a NTR-Sensitive Molecular Probe

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1088
Author(s):  
Su Jung Kim ◽  
Jung Won Yoon ◽  
Shin A Yoon ◽  
Min Hee Lee
Keyword(s):  
A549 Cells ◽  
Living Cells ◽  
Nitroaromatic Compounds ◽  
Molecular Probe ◽  
Ratiometric Fluorescence ◽  
Treatment Development ◽  
Fluorescent Molecule ◽  
Color Changes ◽  
Amino Derivatives ◽  
Distinct Increase

Nitroreductases belong to a member of flavin-containing enzymes that can reduce nitroaromatic compounds to amino derivatives with NADH as an electron donor. NTR activity is known to be elevated in the cancerous environment and is considered an advantageous target in therapeutic prodrugs for the treatment of cancer. Here, we developed a ratiometric fluorescent molecule for observing NTR activity in living cells. This can provide a selective and sensitive response to NTR with a distinct increase in fluorescence ratio (FI530/FI630) as well as color changes. We also found a significant increase in NTR activity in cervical cancer HeLa and lung cancer A549 cells compared to non-cancerous NIH3T3. We proposed that this new ratiometric fluorescent molecule could potentially be used as a NTR-sensitive molecular probe in the field of cancer diagnosis and treatment development related to NTR activity.

Ratiometric fluorescence probe for two-photon bioimaging of Cr3+ in living cells

2014 ◽  
Vol 55 (30) ◽  
pp. 4075-4077 ◽  
Author(s):  
Zhiguo Zhou ◽  
Ying Li ◽  
Yongquan Wu
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Two Photon

A ratiometric fluorescence sensor for HOCl based on a FRET platform and application in living cells

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17059-17063 ◽  
Author(s):  
Yan-Ru Zhang ◽  
Zhi-Min Zhao ◽  
Le Su ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescence Sensor ◽  
Ratiometric Fluorescence ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe CRSH based on a FRET platform for detecting HOCl. CRSH showed high selectivity, excellent sensitivity and a fast response toward HOCl.

scholarly journals A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Qixin Chen ◽  
Hongbao Fang ◽  
Xintian Shao ◽  
Zhiqi Tian ◽  
Shanshan Geng ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Molecular Probe ◽  
Live Cells ◽  
Blue Fluorescence ◽  
Red Fluorescence ◽  
Dynamic Tracking ◽  
A Cell

AbstractMitochondria–lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red–blue fluorescence intensity indicates the progress of mitochondria–lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria–lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria–lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria–lysosome interactions in living cells.

Single-excitation, dual-emission biomass quantum dots: preparation and application for ratiometric fluorescence imaging of coenzyme A in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9270-9275 ◽  
Author(s):  
Yong Cui ◽  
Rongjun Liu ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Coenzyme A ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Dual Emissions

A distinctive approach is proposed to prepare biomass quantum dots (BQDs) with single-excitation and dual-emissions property. The obtained BQDs have been employed as the fluorescence probes for ratiometric fluorescence detection and imaging of coenzyme A in living cells.

scholarly journals A ratiometric fluorescence probe for imaging sulfur dioxide derivatives in the mitochondria of living cells

2017 ◽  
Vol 15 (13) ◽  
pp. 2734-2739 ◽  
Author(s):  
Yongfei Wang ◽  
Qingtao Meng ◽  
Run Zhang ◽  
Hongmin Jia ◽  
Xuehu Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Sulfur Dioxide ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence

The fluorescence probe, NBD-Id, was designed and synthesized for the detection of SO2 derivatives in 100% aqueous solution and living cells.

Real-Time Visualization of Cysteine Metabolism in Living Cells with Ratiometric Fluorescence Probes

2018 ◽  
Vol 90 (4) ◽  
pp. 2686-2691 ◽  
Author(s):  
Bingying Xu ◽  
Haibo Zhou ◽  
Qingsong Mei ◽  
Wei Tang ◽  
Yilun Sun ◽  
...  
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Ratiometric Fluorescence ◽  
Cysteine Metabolism ◽  
Real Time Visualization
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