scholarly journals The Early Adhesion Effects of Human Gingival Fibroblasts on Bovine Serum Albumin Loaded Hydrogenated Titanium Nanotube Surface

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5229
Author(s):  
Yuchen Sun ◽  
Ran Lu ◽  
Jingming Liu ◽  
Xin Wang ◽  
Haitao Dong ◽  
...  

The soft tissue sealing at the transmucal portion of implants is vital for the long-term stability of implants. Hydrogenated titanium nanotubes (H2-TNTs) as implant surface treatments were proved to promote the adhesion of human gingival fibroblasts (HGFs) and have broad usage as drug delivery systems. Bovine serum albumin (BSA) as the most abundant albumin in body fluid was crucial for cell adhesion and was demonstrated as a normal loading protein. As the first protein arriving on the surface of the implant, albumin plays an important role in initial adhesion of soft tissue cells, it is also a common carrier, transferring and loading different endogenous and exogenous substances, ions, drugs, and other small molecules. The aim of the present work was to investigate whether BSA-loaded H2-TNTs could promote the early adhesion of HGFs; H2-TNTs were obtained by hydrogenated anodized titanium dioxide nanotubes (TNTs) in thermal treatment, and BSA was loaded in the nanotubes by vacuum drying; our results showed that the superhydrophilicity of H2-TNTs is conducive to the loading of BSA. In both hydrogenated titanium nanotubes and non-hydrogenated titanium nanotubes, a high rate of release was observed over the first hour, followed by a period of slow and sustained release; however, BSA-loading inhibits the early adhesion of human gingival fibroblasts, and H2-TNTs has the best promoting effect on cell adhesion. With the release of BSA after 4 h, the inhibitory effect of BSA on cell adhesion was weakened.

2015 ◽  
Vol 356 ◽  
pp. 844-851 ◽  
Author(s):  
Bing Cai ◽  
Kebang Hu ◽  
Chunming Li ◽  
Jing Jin ◽  
Yuexin Hu

Langmuir ◽  
2013 ◽  
Vol 29 (21) ◽  
pp. 6429-6433 ◽  
Author(s):  
Ryuzo Kawamura ◽  
Mari Mishima ◽  
Seunghwan Ryu ◽  
Yu Arai ◽  
Motomu Okose ◽  
...  

2012 ◽  
Vol 529-530 ◽  
pp. 365-369 ◽  
Author(s):  
Jumpei Hayashi ◽  
Kawashita Masakazu ◽  
Toshiki Miyazaki ◽  
Tada Aki Kudo ◽  
Hiroyasu Kanetaka ◽  
...  

MC3T3-E1 cell responses, such as cell adhesion and proliferation, to original and bovine serum albumin (BSA) coated disc (original-disc, BSA-disc) of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) was studied. There was no significant difference in the cell proliferation between BSA-discs and original-discs even after incubated for 14 days, but the cell number at day 14 tended to be higher (p = 0.054) on the BSA-discs of HA than on the original-discs of HA. Incidentally, the amount of adsorbed protein was higher on BSA-discs than on original-discs only until incubated in culture medium for 3 h. BSA adsorption might influence the MC3T3-E1 cell adhesion to HA, as a result the specific adsorption of albumin on HA is likely to affect the expression of the osteoconductivity of materials.


RSC Advances ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 6200-6212 ◽  
Author(s):  
Zhen Yang ◽  
Mingyue Liu ◽  
Yang Yang ◽  
Miao Zheng ◽  
Yang Yang ◽  
...  

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine and its effect on HGFs/bacterial adhesion for enhanced soft tissue seal.


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