scholarly journals Methyl Jasmonate Effect on Betulinic Acid Content and Biological Properties of Extract from Senna obtusifolia Transgenic Hairy Roots

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6208
Author(s):  
Tomasz Kowalczyk ◽  
Przemysław Sitarek ◽  
Anna Merecz-Sadowska ◽  
Monika Szyposzyńska ◽  
Aleksandra Spławska ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Betulinic Acid ◽  
Topoisomerase I ◽  
Health Effect ◽  
Biological Properties ◽  
Chromosomal Dna ◽  
Pentacyclic Triterpene ◽  
Senna Obtusifolia ◽  
Transgenic Hairy Roots

It is known that Senna obtusifolia has been used in medicine since ancient times due to the content of many valuable compounds with a pro-health effect. One of them is betulinic acid, which is a pentacyclic triterpene with antimalarial, antiviral, anti-inflammatory and anticancer properties. In this work, a continuation of our previous research, an attempt was made to increase the level of betulinic acid accumulation by the cultivation of transgenic hairy roots that overexpress the squalene synthase gene in a 10 L sprinkle bioreactor with methyl jasmonate elicitation. We present that the applied strategy allowed us to increase the content of betulinic acid in hairy root cultures to the level of 48 mg/g dry weight. The obtained plant extracts showed a stronger cytotoxic effect on the U87MG glioblastoma cell line than the roots grown without elicitors. Additionally, the induction of apoptosis, reduction of mitochondrial membrane potential, chromosomal DNA fragmentation and activation of caspase cascades are demonstrated. Moreover, the tested extract showed inhibition of topoisomerase I activity.

scholarly journals An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 510 ◽  
Author(s):  
Tomasz Kowalczyk ◽  
Przemysław Sitarek ◽  
Monika Toma ◽  
Laurent Picot ◽  
Marzena Wielanek ◽  
...  
Keyword(s):  
Cell Line ◽  
Hairy Root ◽  
Hairy Roots ◽  
Betulinic Acid ◽  
Lymphoblastic Leukemia ◽  
Leukemia Cell ◽  
Biologically Active ◽  
Leukemia Cell Line ◽  
Root Extract ◽  
Senna Obtusifolia

Many biologically-active plant-derived compounds have therapeutic or chemopreventive effects. The use of plant in vitro cultures in conjunction with modern genetic engineering techniques allows greater amounts of valuable secondary metabolites to be obtained without interfering with the natural environment. This work presents the first findings concerning the acquisition of transgenic hairy roots of Senna obtusifolia overexpressing the gene encoding squalene synthase 1 from Panax ginseng (PgSS1) (SOPSS hairy loot lines) involved in terpenoid biosynthesis. Our results confirm that one of PgSS1-overexpressing hairy root line extracts (SOPSS2) possess a high cytotoxic effect against a human acute lymphoblastic leukemia (NALM6) cell line. Further analysis of the cell cycle, the expression of apoptosis-related genes (TP53, PUMA, NOXA, BAX) and the observed decrease in mitochondrial membrane potential also confirmed that the SOPSS2 hairy root extract displays the highest effects; similar results were also obtained for this extract combined with doxorubicin. The high cytotoxic activity, observed both alone or in combination with doxorubicin, may be due to the higher content of betulinic acid as determined by HPLC analysis. Our results suggest synergistic effects of tested extract (betulinic acid in greater amount) with doxorubicin which may be used in the future to develop new effective strategies of cancer chemosensitization.

scholarly journals Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 975
Author(s):  
Ye-Eun Park ◽  
Chang-Ha Park ◽  
Hyeon-Ji Yeo ◽  
Yong-Suk Chung ◽  
Sang-Un Park
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Root ◽  
Hairy Roots ◽  
Seed Coat ◽  
Biological Properties ◽  
Fresh Weight ◽  
Good Source ◽  
Plant Parts ◽  
Seedling Roots

Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.

scholarly journals Erratum: Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate [Chem. Pharm. Bull. 58(7): 934-938 (2010)]

2010 ◽  
Vol 58 (9) ◽  
pp. 1281
Author(s):  
Moriyuki Kawauchi ◽  
Toshihide Arima ◽  
Osamu Shirota ◽  
Setsuko Sekita ◽  
Takahisa Nakane ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Roots ◽  
Hyoscyamus Albus

Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

2008 ◽  
Vol 19 (1) ◽  
pp. 76-78 ◽  
Author(s):  
Rong Min Yu ◽  
Liang Bin Zhou ◽  
Chun Yan Yan ◽  
Guo Yan Duan ◽  
Yu Zhao
Keyword(s):  
Hairy Roots ◽  
Polygonum Multiflorum ◽  
Transgenic Hairy Roots

scholarly journals Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida

1995 ◽  
Vol 8 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Peter E. Urwin ◽  
Howard J. Atkinson ◽  
David A. Waller ◽  
Michael J. McPherson
Keyword(s):  
Hairy Roots ◽  
Globodera Pallida ◽  
Transgenic Hairy Roots

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

scholarly journals Chemoenzymatic Synthesis of Two New Halogenated Coumarin Glycosides as Potential Antifungal Agents

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Liangbin Zhou ◽  
Ling Liu ◽  
Tian Tian ◽  
Bailin Xue ◽  
Rongmin Yu
Keyword(s):  
Hairy Roots ◽  
Antifungal Agents ◽  
Chemoenzymatic Synthesis ◽  
Polygonum Multiflorum ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Transgenic Hairy Roots ◽  
Exogenous Sugar

Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7- O- β-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5- O- β-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.

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