scholarly journals Near-Infrared Spectroscopy (NIRS) as a Method for Biological Sex Discrimination in the Endangered Houston Toad (Anaxyrus houstonensis)

2021 ◽  
Vol 5 (1) ◽  
pp. 4
Author(s):  
Li-Dunn Chen ◽  
Mariana Santos-Rivera ◽  
Isabella J. Burger ◽  
Andrew J. Kouba ◽  
Diane M. Barber ◽  
...  

Biological sex is one of the more critically important physiological parameters needed for managing threatened animal species because it is crucial for informing several of the management decisions surrounding conservation breeding programs. Near-infrared spectroscopy (NIRS) is a non-invasive technology that has been recently applied in the field of wildlife science to evaluate various aspects of animal physiology and may have potential as an in vivo technique for determining biological sex in live amphibian species. This study investigated whether NIRS could be used as a rapid and non-invasive method for discriminating biological sex in the endangered Houston toad (Anaxyrus houstonensis). NIR spectra (N = 396) were collected from live A. houstonensis individuals (N = 132), and distinct spectral patterns between males and females were identified using chemometrics. Linear discriminant analysis (PCA-LDA) classified the spectra from each biological sex with accuracy ≥ 98% in the calibration and internal validation datasets and 94% in the external validation process. Through the use of NIRS, we have determined that unique spectral signatures can be holistically captured in the skin of male and female anurans, bringing to light the possibility of further application of this technique for juveniles and sexually monomorphic species, whose sex designation is important for breeding-related decisions.

2019 ◽  
pp. 346-352 ◽  
Author(s):  
John-Lewis Zaukuu ◽  
Zsanett Bodor ◽  
Flora Vitalis ◽  
Victoria Zsom-Muha ◽  
Zoltan Kovacs

Paprika powder is a spice of culinary importance in many homes but it?s powdered form, has been targeted for fraudulent activities intended at consumer deception. Diverse methods have been used to investigate some of these adulterations but there is no report of paprika adulteration with corn flour, although it remains a suspicion. Technologies such as the near infrared spectroscopy (NIRS) possess non-invasive and rapid advantages that could be explored to monitor this type of adulteration. The study aimed to discriminate and quantify different levels of paprika powder adulterated with corn flour, using NIRS. Two authentic paprika types (DP and SP) were purchased from reputable sources in Hungary and artificially adulterated in the laboratory. Three repeats of each adulteration level (40%, 30%, 25%, 20%, 15%, 10%, 5%, 3%, 1%) were prepared and scanned with the Metri NIRS respectively, then, analysed with chemometrics: Linear discriminant analysis (LDA) and partial least squares regression (PLSR). LDA showed 100% recognition and prediction accuracies respectively when DP and SP were analyzed separately to discriminate different concentrations of paprika adulteration. LDA models with NIRS recognize corn flour adulteration with 95.55% and predict it with 95.02% accuracy irrespective of the paprika type used in this experiment. PLSR prediction of 40%, 30%, 25%, 20%, 15%, 10%, 5%, 3%, 1% corn flour adulteration yielded an R2CV of 0.98 (high accuracy) and a low RMSECV of 1.71 g/100g (low error). Near infrared as a non-invasive technique exhibited good potentials for paprika powder authentication when corn flour is used as an adulterant.


2021 ◽  
Vol 11 (23) ◽  
pp. 11379
Author(s):  
Alberto Ortiz ◽  
Lucía León ◽  
Rebeca Contador ◽  
David Tejerina

The ability of Near Infrared Spectroscopy (NIRS) to classify pre-sliced Iberian chorizo modified atmosphere packaged (MAP) according to the animal material used in their production (Black, Red, White) in their production in accordance with the official trade categories (which includes the handling system and the different inter-racial crossbreeds) without opening the package was assayed. Furthermore, various spectra pre-treatments and supervised classification chemometric tools; Partial least square-discriminant analysis (PLS-DA), soft independent modelling of class analogies (SIMCA) and linear discriminant analysis (LDA), were assessed. The highest sensitivity values in both calibration and external validation were achieved with SIMCA followed by PLS-DA approaches, while LDA had more provided values among sensitivity and specificity and between the different commercial categories in both sample sets, thus yielding the highest discriminant ability. These results could be a resource to support the traceability and authentication control of individual pre-sliced MAP Iberian chorizo according to the commercial category of the raw material in a non-destructive way.


2021 ◽  
Vol 3 (1) ◽  
pp. 73-91
Author(s):  
João Serrano ◽  
Shakib Shahidian ◽  
Ângelo Carapau ◽  
Ana Elisa Rato

Dryland pastures provide the basis for animal sustenance in extensive production systems in Iberian Peninsula. These systems have temporal and spatial variability of pasture quality resulting from the diversity of soil fertility and pasture floristic composition, the interaction with trees, animal grazing, and a Mediterranean climate characterized by accentuated seasonality and interannual irregularity. Grazing management decisions are dependent on assessing pasture availability and quality. Conventional analytical determination of crude protein (CP) and fiber (neutral detergent fiber, NDF) by reference laboratory methods require laborious and expensive procedures and, thus, do not meet the needs of the current animal production systems. The aim of this study was to evaluate two alternative approaches to estimate pasture CP and NDF, namely one based on near-infrared spectroscopy (NIRS) combined with multivariate data analysis and the other based on the Normalized Difference Vegetation Index (NDVI) measured in the field by a proximal active optical sensor (AOS). A total of 232 pasture samples were collected from January to June 2020 in eight fields. Of these, 96 samples were processed in fresh form using NIRS. All 232 samples were dried and subjected to reference laboratory and NIRS analysis. For NIRS, fresh and dry samples were split in two sets: a calibration set with half of the samples and an external validation set with the remaining half of the samples. The results of this study showed significant correlation between NIRS calibration models and reference methods for quantifying pasture quality parameters, with greater accuracy in dry samples (R2 = 0.936 and RPD = 4.01 for CP and R2 = 0.914 and RPD = 3.48 for NDF) than fresh samples (R2 = 0.702 and RPD = 1.88 for CP and R2 = 0.720 and RPD = 2.38 for NDF). The NDVI measured by the AOS shows a similar coefficient of determination to the NIRS approach with pasture fresh samples (R2 = 0.707 for CP and R2 = 0.648 for NDF). The results demonstrate the potential of these technologies for estimating CP and NDF in pastures, which can facilitate the farm manager’s decision making in terms of the dynamic management of animal grazing and supplementation needs.


2016 ◽  
Vol 56 (9) ◽  
pp. 1504 ◽  
Author(s):  
J. P. Keim ◽  
H. Charles ◽  
D. Alomar

An important constraint of in situ degradability studies is the need to analyse a high number of samples and often with insufficient amount of residue, especially after the longer incubations of high-quality forages, that impede the study of more than one nutritional component. Near-infrared spectroscopy (NIRS) has been established as a reliable method for predicting composition of many entities, including forages and other animal feedstuffs. The objective of this work was to evaluate the potential of NIRS for predicting the crude protein (CP) and neutral detergent fibre (NDF) concentration in rumen incubation residues of permanent and sown temperate pastures in a vegetative stage. In situ residues (n = 236) from four swards were scanned for their visible-NIR spectra and analysed for CP and NDF. Selected equations developed by partial least-squares multivariate regression presented high coefficients of determination (CP = 0.99, NDF = 0.95) and low standard errors (CP = 4.17 g/kg, NDF = 7.91 g/kg) in cross-validation. These errors compare favourably to the average concentrations of CP and NDF (146.5 and 711.2 g/kg, respectively) and represent a low fraction of their standard deviation (CP = 38.2 g/kg, NDF = 34.4 g/kg). An external validation was not as successful, with R2 of 0.83 and 0.82 and a standard error of prediction of 14.8 and 15.2 g/kg, for CP and NDF, respectively. It is concluded that NIRS has the potential to predict CP and NDF of in situ incubation residues of leafy pastures typical of humid temperate zones, but more robust calibrations should be developed.


2010 ◽  
Vol 03 (01) ◽  
pp. 69-74 ◽  
Author(s):  
YE ZHU ◽  
TIANZI JIANG ◽  
YUAN ZHOU ◽  
LISHA ZHAO

Functional near-infrared spectroscopy (fNIRS) is a neuroimaging technology which is suitable for psychiatric patients. Several fNIRS studies have found abnormal brain activations during cognitive tasks in elderly depression. In this paper, we proposed a discriminative model of multivariate pattern classification based on fNIRS signals to distinguish elderly depressed patients from healthy controls. This model used the brain activation patterns during a verbal fluency task as features of classification. Then Pseudo-Fisher Linear Discriminant Analysis was performed on the feature space to generate discriminative model. Using leave-one-out (LOO) cross-validation, our results showed a correct classification rate of 88%. The discriminative model showed its ability to identify people with elderly depression and suggested that fNIRS may be an efficient clinical tool for diagnosis of depression. This study may provide the first step for the development of neuroimaging biomarkers based on fNIRS in psychiatric disorders.


2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Noman Naseer ◽  
Nauman Khalid Qureshi ◽  
Farzan Majeed Noori ◽  
Keum-Shik Hong

We analyse and compare the classification accuracies of six different classifiers for a two-class mental task (mental arithmetic and rest) using functional near-infrared spectroscopy (fNIRS) signals. The signals of the mental arithmetic and rest tasks from the prefrontal cortex region of the brain for seven healthy subjects were acquired using a multichannel continuous-wave imaging system. After removal of the physiological noises, six features were extracted from the oxygenated hemoglobin (HbO) signals. Two- and three-dimensional combinations of those features were used for classification of mental tasks. In the classification, six different modalities, linear discriminant analysis (LDA), quadratic discriminant analysis (QDA),k-nearest neighbour (kNN), the Naïve Bayes approach, support vector machine (SVM), and artificial neural networks (ANN), were utilized. With these classifiers, the average classification accuracies among the seven subjects for the 2- and 3-dimensional combinations of features were 71.6, 90.0, 69.7, 89.8, 89.5, and 91.4% and 79.6, 95.2, 64.5, 94.8, 95.2, and 96.3%, respectively. ANN showed the maximum classification accuracies: 91.4 and 96.3%. In order to validate the results, a statistical significance test was performed, which confirmed that thepvalues were statistically significant relative to all of the other classifiers (p< 0.005) using HbO signals.


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