Micromixer Synthesis Platform for a Tuneable Production of Magnetic Single-Core Iron Oxide Nanoparticles

Micromixer technology is a novel approach to manufacture magnetic single-core iron oxide nanoparticles that offer huge potential for biomedical applications. This platform allows a continuous, scalable, and highly controllable synthesis of magnetic nanoparticles with biocompatible educts via aqueous synthesis route. Since each biomedical application requires specific physical and chemical properties, a comprehensive understanding of the synthesis mechanisms is not only mandatory to control the size and shape of desired nanoparticle systems but, above all, to obtain the envisaged magnetic particle characteristics. The accurate process control of the micromixer technology can be maintained by adjusting two parameters: the synthesis temperature and the residence time. To this end, we performed a systematic variation of these two control parameters synthesizing magnetic nanoparticle systems, which were analyzed afterward by structural (transmission electron microscopy and differential sedimentation centrifugation) and, especially, magnetic characterization methods (magnetic particle spectroscopy and AC susceptibility). Furthermore, we investigated the reproducibility of the microtechnological nanoparticle manufacturing process compared to batch preparation. Our characterization demonstrated the high magnetic quality of single-core iron oxide nanoparticles with core diameters in the range of 20 nm to 40 nm synthesized by micromixer technology. Moreover, we demonstrated the high capability of a newly developed benchtop magnetic particle spectroscopy device that directly monitored the magnetic properties of the magnetic nanoparticles with the highest sensitivity and millisecond temporal resolution during continuous micromixer synthesis.

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Evaluation of Surface-modified Superparamagnetic Iron Oxide Nanoparticles to Optimize Bacterial Immobilization for Bio-separation with the Least Inhibitory Effect on Microorganism Activity

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681208666181015120346 ◽

2020 ◽

Vol 10 (2) ◽

pp. 166-174

Author(s):

Mehdi Khoshneviszadeh ◽

Sarah Zargarnezhad ◽

Younes Ghasemi ◽

Ahmad Gholami

Keyword(s):

Iron Oxide ◽

Amino Acid ◽

Magnetic Nanoparticles ◽

Iron Oxide Nanoparticles ◽

Surface Coating ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Surface Charges ◽

Surface Modified

Background: Magnetic cell immobilization has been introduced as a novel, facile and highly efficient approach for cell separation. A stable attachment between bacterial cell wall with superparamagnetic iron oxide nanoparticles (SPIONs) would enable the microorganisms to be affected by an outer magnetic field. At high concentrations, SPIONs produce reactive oxygen species in cytoplasm, which induce apoptosis or necrosis in microorganisms. Choosing a proper surface coating could cover the defects and increase the efficiency. Methods: In this study, asparagine, APTES, lipo-amino acid and PEG surface modified SPIONs was synthesized by co-precipitation method and characterized by FTIR, TEM, VSM, XRD, DLS techniques. Then, their protective effects against four Gram-positive and Gram-negative bacterial strains including Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were examined through microdilution broth and compared to naked SPION. Results: The evaluation of characterization results showed that functionalization of magnetic nanoparticles could change their MS value, size and surface charges. Also, the microbial analysis revealed that lipo-amino acid coated magnetic nanoparticles has the least adverse effect on microbial strain among tested SPIONs. Conclusion: This study showed lipo-amino acid could be considered as the most protective and even promotive surface coating, which is explained by its optimizing effect on cell penetration and negligible reductive effects on magnetic properties of SPIONs. lipo-amino acid coated magnetic nanoparticles could be used in microbial biotechnology and industrial microbiology.

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Effect of aluminum doped iron oxide nanoparticles on magnetic properties of the polyacrylonitrile nanofibers

Journal of Polymer Engineering ◽

10.1515/polyeng-2015-0303 ◽

2017 ◽

Vol 37 (2) ◽

pp. 135-141

Author(s):

Armin Ourang ◽

Soheil Pilehvar ◽

Mehrzad Mortezaei ◽

Roya Damircheli

Keyword(s):

Magnetic Properties ◽

Iron Oxide ◽

Magnetic Nanoparticles ◽

Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Negative Interaction ◽

Magnetic Nanofibers

Abstract In this work, polyacrylonitrile (PAN) was electrospun with and without magnetic nanoparticles (aluminum doped iron oxide) and was turned into magnetic nanofibers. The results showed that nanofibers diameter decreased from 700 nm to 300 nm by adding nanoparticles. Furthermore, pure PAN nanofibers were indicated to have low magnetic ability due to polar bonds that exist in their acrylonitrile groups. Obviously by adding only 4 wt% of the nanoparticles to PAN nanofibers, magnetic ability soared by more than 10 times, but at a higher percentage, it was shown to change just a little due to negative interaction among nanoparticles. This event relates to antiferromagnetically coupling of nanoparticles due to incomplete dispersion at higher percentage.

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Changes in the behavior of magnetic iron oxide nanoparticles during cellular uptake detected by magnetic particle spectroscopy

2015 5th International Workshop on Magnetic Particle Imaging (IWMPI) ◽

10.1109/iwmpi.2015.7107009 ◽

2015 ◽

Author(s):

F. Wiekhorst ◽

N. Lowa ◽

W. Poller ◽

S. Metzkow ◽

A. Ludwig ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Cellular Uptake ◽

Magnetic Particle ◽

Oxide Nanoparticles ◽

Magnetic Iron Oxide Nanoparticles ◽

Magnetic Iron Oxide

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The Effect of Focused Ultrasound on Magnetic Polyelectrolyte Capsules Loaded with Dye When Suspended in Tissue-Mimicking Gel

Current Drug Delivery ◽

10.2174/1567201816666190103121313 ◽

2019 ◽

Vol 16 (4) ◽

pp. 355-363 ◽

Cited By ~ 2

Author(s):

Carmen Stavarache ◽

Mircea Vinatoru ◽

Timothy Mason

Keyword(s):

Iron Oxide ◽

Magnetic Nanoparticles ◽

Iron Oxide Nanoparticles ◽

Exposure Time ◽

Ultrasonic Irradiation ◽

Focused Ultrasound ◽

Relative Degree ◽

Oxide Nanoparticles ◽

Dye Release ◽

Iron Oxide Magnetic Nanoparticles

Background: Capsules containing a dye were prepared by the LbL method with iron oxide nanoparticles (50 nm) in different layers of the shell. Method: The capsules were dispersed in a gel and subjected to focused ultrasonic irradiation at three different powers and exposure times. Result: It was found that the inclusion of iron oxide magnetic nanoparticles in any of the polyelectrolyte shells (4, 6, 8 and 10) strengthened the capsules with respect to capsules without nanoparticles. Incorporation of nanoparticles in shell 8 provided the most resistance to fragmentation under focused ultrasonic irradiation. The relative degree of capsule stability is dependent on both the power of the ultrasound and the exposure time. Conclusion: The presence of iron oxide nanoparticles not only conferred more resistance to fragmentation but also provided a route to protein labelled dye release through sonoporation that was not present for capsules without nanoparticles.

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Preparation and characterization of bio-functionalized iron oxide nanoparticles for biomedical application

Thin Solid Films ◽

10.1016/j.tsf.2010.08.072 ◽

2010 ◽

Vol 519 (3) ◽

pp. 1219-1223 ◽

Cited By ~ 16

Author(s):

Bharat Bajaj ◽

B.D. Malhotra ◽

Sunju Choi

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Biomedical Application ◽

Oxide Nanoparticles

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Intracellular dynamics of superparamagnetic iron oxide nanoparticles for magnetic particle imaging

Nanoscale ◽

10.1039/c9nr01395d ◽

2019 ◽

Vol 11 (16) ◽

pp. 7771-7780 ◽

Cited By ~ 14

Author(s):

Eric Teeman ◽

Carolyn Shasha ◽

James E. Evans ◽

Kannan M. Krishnan

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Environmental Conditions ◽

Magnetic Particle ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Magnetic Response ◽

Magnetic Particle Imaging ◽

Particle Imaging

An examination of the effects of intracellular environmental conditions on the dynamic magnetic response of superparamagnetic iron oxide nanoparticles.

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