scholarly journals Daily Oral Supplementation with 60 mg of Elemental Iron for 12 Weeks Alters Blood Mitochondrial DNA Content, But Not Leukocyte Telomere Length in Cambodian Women

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1877
Author(s):  
Shannon L. Steele ◽  
Anthony Y. Y. Hsieh ◽  
Izabella Gadawski ◽  
Hou Kroeun ◽  
Susan I. Barr ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Telomere Length ◽  
Iron Supplementation ◽  
Quantitative Polymerase Chain Reaction ◽  
Cellular Damage ◽  
Leukocyte Telomere Length ◽  
Oral Supplementation ◽  
Elemental Iron ◽  
Mitochondrial Homeostasis ◽  
Percent Change

There is limited evidence regarding the potential risk of untargeted iron supplementation, especially among individuals who are iron-replete or have genetic hemoglobinopathies. Excess iron exposure can increase the production of reactive oxygen species, which can lead to cellular damage. We evaluated the effect of daily oral supplementation on relative leukocyte telomere length (rLTL) and blood mitochondrial DNA (mtDNA) content in non-pregnant Cambodian women (18–45 years) who received 60 mg of elemental iron as ferrous sulfate (n = 190) or a placebo (n = 186) for 12 weeks. Buffy coat rLTL and mtDNA content were quantified by monochrome multiplex quantitative polymerase chain reaction. Generalized linear mixed-effects models were used to predict the absolute and percent change in rLTL and mtDNA content after 12 weeks. Iron supplementation was not associated with an absolute or percent change in rLTL after 12 weeks compared with placebo (ß-coefficient: −0.04 [95% CI: −0.16, 0.08]; p = 0.50 and ß-coefficient: −0.96 [95% CI: −2.69, 0.77]; p = 0.28, respectively). However, iron supplementation was associated with a smaller absolute and percent increase in mtDNA content after 12 weeks compared with placebo (ß-coefficient: −11 [95% CI: −20, −2]; p = 0.02 and ß-coefficient: −11 [95% CI: −20, −1]; p= 0.02, respectively). Thus, daily oral iron supplementation for 12 weeks was associated with altered mitochondrial homeostasis in our study sample. More research is needed to understand the risk of iron exposure and the biological consequences of altered mitochondrial homeostasis in order to inform the safety of the current global supplementation policy.

scholarly journals Telomere Length in Patients with Transfusion-Dependent Thalassemia

2020 ◽  
Author(s):  
Nithita Nanthatanti ◽  
Adisak Tantiworawit ◽  
Pokpong Piriyakhuntorn ◽  
Thanawat Rattanathammethee ◽  
Sasinee Hantrakool ◽  
...  
Keyword(s):  
Iron Overload ◽  
Telomere Length ◽  
Major Complication ◽  
Single Copy ◽  
Cross Sectional Study ◽  
Hemoglobin Level ◽  
Cellular Damage ◽  
Repair System ◽  
Leukocyte Telomere Length ◽  
Cross Sectional

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non-transfusion dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the leukocyte telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ≥18 years. Leukocyte telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin level was 7.1±1.07 g/dL. The mean telomere to single copy gene (T/S) ratios of patients with TDT and the controls were 0.72±0.18 and 0.99±0.25, respectively (p <0.0001). There was a significant correlation between the T/S ratio and age (p = 0.0002), and hemoglobin level (p = 0.044). There was no correlation between telomere length and other factors. Conclusions: Our study showed that TDT patients had shorter leukocyte telomere length compared with controls. Leukocyte telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

scholarly journals Association Between Dietary Selenium Intake and Leukocyte Telomere Length in a Nationally Representative Sample of US Adults (OR11-06-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Buyun Liu ◽  
Yangbo Sun ◽  
Guifeng Xu ◽  
Shuang Rong ◽  
Wei Bao
Keyword(s):  
Telomere Length ◽  
Representative Sample ◽  
Quantitative Polymerase Chain Reaction ◽  
Antioxidant Properties ◽  
Biological Aging ◽  
Leukocyte Telomere Length ◽  
Telomere Shortening ◽  
Dietary Selenium ◽  
Selenium Intake ◽  
Nationally Representative

Abstract Objectives DNA damage induced by oxidative stress is implicated in accelerated telomere shortening, a biomarker of biological aging. Although selenium has antioxidant properties, its impact on telomere length is largely unknown. This study aimed to examine the association between dietary selenium intake and leukocyte telomere length in a nationally representative sample of US adults. Methods We included 7409 adults aged 20 years or older who participated in the National Health and Nutrition Examination Survey (NHANES) 1999–2002. Dietary selenium intake was calculated using data collected in the 24-hour dietary recall. Leukocyte telomere length was assayed using the quantitative polymerase chain reaction method. The association between selenium intake and telomere length was estimated by weighted linear regression models adjusting for demographic, socioeconomic and lifestyle factors, body mass index, supplements intake, and leukocyte cell type composition. Results The average dietary selenium intake was 109.1 mg/d (standard error [SE] 1.15). We didn't find a significant association between dietary selenium intake and telomere length in US adults. The average telomere length (SE) was 1.01 (0.02), 1.01 (0.01), and 1.04 (0.01) across increasing tertiles of dietary selenium intake. However, a significant interaction was observed for age (P = 0.02). Among individuals aged 20–44 years, the β coefficient of log-transformed telomere length, compared to lowest tertile of dietary selenium intake, was −0.041 (SE 0.012, P = 0.002) and −0.033 (SE 0.018, P = 0.07) for middle tertile and the highest tertile of selenium intake, respectively. The corresponding β coefficient was 0.009 (SE 0.016, P = 0.59) and −0.001 (SE 0.012, P = 0.95), respectively, for adults 45–64 years old, and 0.017 (SE 0.015, P = 0.28) and 0.059 (SE 0.021, P = 0.01), respectively, for those aged 65 years or older. The results were not appreciably changed even after additionally adjustment for dietary intake of vitamin A, vitamin E, and zinc. Conclusions The association between dietary selenium intake and telomere length differed significantly by age groups, indicating that higher selenium intake may prevent telomere shortening in older adults but not in younger or middle-aged adults. Further studies about the underlying mechanisms are warranted. Funding Sources NA.

Association of Leukocyte Telomere Length and Mitochondrial DNA Copy Number in Children from Salamanca, Mexico

2016 ◽  
Vol 20 (11) ◽  
pp. 654-659 ◽  
Author(s):  
Jorge Alejandro Alegría-Torres ◽  
Marion Velázquez-Villafaña ◽  
Juan Manuel López-Gutiérrez ◽  
Marcela M. Chagoyán-Martínez ◽  
Diana O. Rocha-Amador ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Telomere Length ◽  
Copy Number ◽  
Leukocyte Telomere Length ◽  
Dna Copy Number ◽  
Mitochondrial Dna Copy Number

Abstract P213: Association Between Depression and Leukocyte Telomere Length in Patients With Stable Coronary Heart Disease: Data From the Heart and Soul Study

2011 ◽  
Vol 4 (suppl_1) ◽  
Author(s):  
Petra Hoen ◽  
Peter de Jonge ◽  
Beeya Na ◽  
Ramin Farzaneh-Far ◽  
Elissa Epel ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Major Depressive Disorder ◽  
Heart Disease ◽  
Major Depression ◽  
Depressive Disorder ◽  
Telomere Length ◽  
Quantitative Polymerase Chain Reaction ◽  
Leukocyte Telomere Length ◽  
Major Depressive ◽  
Stable Coronary Heart Disease

Background Shortened telomere length has been associated with mortality in patients with coronary heart disease (CHD) and is considered an emerging marker of biological age. Whether short telomere length is associated with depression in patients with CHD has not been evaluated. Methods In a cross-sectional study of 952 outpatients with stable CHD, we ascertained the presence of major depressive disorder using the Computerized Diagnostic Interview Schedule. Relative mean telomere length was measured from leukocyte DNA using a quantitative polymerase chain reaction assay. We examined the association between depression and leukocyte telomere length using linear and logistic regression models. Results Of the 952 participants, 206 (22%) had current (past month) depression. Patients with depression had lower mean ± SE telomere length than those without depression ( Table ). Likewise, patients with MDD had a 70% greater odds of having telomere length in the lowest vs. highest quartile (adjusted OR 1.70. 95% CI, 1.05-2.76; p=0.03). Conclusion Major depressive disorder is associated with reduced leukocyte telomere length in patients with stable CHD. The potential mechanisms underlying this association deserve further study. Mean +/- SE telomere length by presence of major depressive disorder Adjusted for Current major depression N=206 No current major depression N=746 P value age, sex 0.86±0.02 0.90±0.1 .02 age, sex, diabetes, BMI, smoking 0.86±0.02 0.89±0.01 .04 age, sex, diabetes, BMI, smoking, LVEF, statin use 0.85±0.02 0.89±0.01 .03

scholarly journals Inverse relationship between leukocyte telomere length attrition and blood mitochondrial DNA content loss over time

Aging ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 15196-15221
Author(s):  
Anthony Y.Y. Hsieh ◽  
Elana Kimmel ◽  
Neora Pick ◽  
Laura Sauvé ◽  
Jason Brophy ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Telomere Length ◽  
Dna Content ◽  
Inverse Relationship ◽  
Leukocyte Telomere Length ◽  
Mitochondrial Dna Content ◽  
Over Time

scholarly journals Leukocyte Telomere Length in Patients with Transfusion-Dependent Thalassemia

2020 ◽  
Author(s):  
Nithita Nanthatanti ◽  
Adisak Tantiworawit ◽  
Pokpong Piriyakhuntorn ◽  
Thanawat Rattanathammethee ◽  
Sasinee Hantrakool ◽  
...  
Keyword(s):  
Iron Overload ◽  
Telomere Length ◽  
Major Complication ◽  
Single Copy ◽  
Cross Sectional Study ◽  
Hemoglobin Level ◽  
Cellular Damage ◽  
Repair System ◽  
Leukocyte Telomere Length ◽  
Cross Sectional

Abstract Background: Thalassemia is a hereditary hemolytic anemia with a severity ranging from mild, non-transfusion dependent to severe chronic anemia requiring lifelong transfusion. Transfusional iron overload is a major complication in patients with transfusion-dependent thalassemia (TDT). Telomeres are sequences of nucleotides forming the end caps of chromosomes that act as a DNA repair system. Iron overload in thalassemia can cause increased oxidative stress which leads to cellular damage and senescence. This may result in telomere length shortening. The degree of telomere length shortening may reflect the severity of thalassemia. Methods: This research aimed to study the leukocyte telomere length in patients with TDT in comparison to non-thalassemic individuals and to identify the clinical and laboratory parameters that are associated with telomere length. We conducted a cross-sectional study in patients with TDT aged ³18 years. Leukocyte telomere length was measured by real-time quantitative PCR. Results: Sixty-five patients with TDT were enrolled onto the study. There were 37 female patients (54.4%). The median age was 27 (18-57) years, and mean pre-transfusion hemoglobin level was 7.1 (± 1.07) g/dL. The mean telomere to single copy gene (T/S) ratios of patients with TDT and the controls were 0.72±0.18 and 0.99±0.25, respectively (p <0.0001). There was a significant correlation between the T/S ratio and age (p = 0.0002), and hemoglobin level (p = 0.044). There was no correlation between telomere length and other factors. Conclusions: Our study showed that TDT patients had shorter leukocyte telomere length compared with controls. Leukocyte telomere shortening in TDT was an aging-dependent process and associated with lower hemoglobin level.

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