scholarly journals Antibiotic Resistance and Plasmid Profile Analysis of Salmonella Enteritidis Isolated in Siberia and the Far East of Russia between 1990 and 2017

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1240
Author(s):  
Alexey V. Rakov ◽  
Natalya A. Kuznetsova
Keyword(s):  
Salmonella Enteritidis ◽  
Profile Analysis ◽  
Molecular Genetic ◽  
Far East ◽  
Plasmid Profile ◽  
Diffusion Method ◽  
Far East Of Russia ◽  
Disk Diffusion Method ◽  
Alkaline Lysis ◽  
The Far East

Salmonella is one of the major causes of foodborne disease outbreaks globally. Specifically, Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the major causes of zoonotic Salmonella infection in humans worldwide. In this study, we present data on antimicrobial resistance (AMR) and plasmid profiles of S. Enteritidis strains isolated from patients, food, and the environment in Siberia and the Far East of Russia obtained during Salmonella monitoring between 1990 and 2017. A total of 345 S. Enteritidis isolates were tested by the disk diffusion method with a set of 15 antibiotics using EUCAST breakpoints v. 10 and by plasmid profile analysis using the alkaline lysis method. The results have shown a substantial decrease in susceptibility to aminoglycosides and quinolones during the study period. No significant differences were found in the susceptibility of strains between regions as well as in the its correlation with different plasmid types of the pathogen. Several S. Enteritidis strains were found to be resistant to ampicillin, kanamycin, tetracycline, chloramphenicol, and cephalosporins. All tested S. Enteritidis strains were susceptible only to imipenem. In this study, we observed a relatively low level of AMR in S. Enteritidis strains isolated in Siberia and the Far East of Russia. Nevertheless, it is important to continue the molecular genetic monitoring and AMR surveillance of S. Enteritidis to track further increases in AMR using conventional phenotypic susceptibility testing and by introducing whole-genome sequencing to identify AMR mechanisms.

scholarly journals Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis

2008 ◽  
Vol 65 (4) ◽  
pp. 303-307
Author(s):  
Biljana Miljkovic-Selimovic ◽  
Zorica Lepsanovic ◽  
Tatjana Babic ◽  
Branislava Kocic ◽  
Gordana Randjelovic
Keyword(s):  
Profile Analysis ◽  
Plasmid Profile ◽  
Geographic Region ◽  
Diffusion Method ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Discrimination Power ◽  
Alkaline Lysis ◽  
Plasmid Profiles ◽  
Correlation Degree ◽  
Sporadic Cases

Background/Aim. As illness caused by Sallmonella enterica serovar Enteritidis (S. Enteritidis) occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. To determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of S. Enteritidis, recovered from patient's stools and food associated with outbreaks and those isolated from sporadic cases of diarrhea, were investigated. Methods. Investigation of antibiotic resistance was performed by Kirby - Bauer disc-diffusion method. Isolation of plasmid DNA was carried out by Birnboim and Dolly alkaline lysis method, modified by Ish-Horovitz. Results. Out of 276 izolates of S. Enteritidis 94 were isolated from patient's stools and food associated with outbreaks and 182 were isolated from sporadic cases of diarrhea. The presence of 12 plasmid profiles was established. An average correlation degree of plasmid profiles between the strains was 0.84, that implies high degree of similarity of plasmid profiles of epidemic and non- epidemic strains isolated at our geographic region for the given period of time. Conclusion. The strains of S. Enteritidis, isolated in outbreaks of enterocolitis as well as from spordic cases of diarrhea in the same period of time and at the same area, frequently exhibit the same plasmid profile characterized by a single plasmid of 38 MDa. Therefore, in most cases plasmid profile analysis is not valuable in the identification of epidemic strains of S. Enteritidis. However, for this purpose plasmid profile analysis could be used when drug-resistant strains of S. Enteritidis are isolated, as they often possess additional resistant plasmids what increases discrimination power of this method.

ECONOMIC SECURITY OF THE FAR EAST OF RUSSIA IN CONDITIONS OF OPERATION OF ADVANCED DEVELOPMENT SYSTEMS

10.22250/esfe ◽  
2020 ◽  
Author(s):  
V. A. YAKIMOVA ◽  
◽  
V.V. LAZAREVA ◽  
V.N. DYACHENKO ◽  
S.V. KHMURA ◽  
...  
Keyword(s):  
Far East ◽  
Economic Security ◽  
Far East Of Russia ◽  
The Far East ◽  
Advanced Development

Two new Palaearctic species of the genus Bucculatrix (Lepidoptera: Bucculatricidae)

2007 ◽  
Vol 16 (2) ◽  
pp. 285-288
Author(s):  
S.V. Baryshnikova
Keyword(s):  
Far East ◽  
Far East Of Russia ◽  
Palaearctic Species ◽  
The Far East ◽  
Middle Asia

Bucculatrix malivorella sp. n. from Middle Asia and B. pectinifera sp. n. from the Far East of Russia are described.

STECCHERINUM AURANTILAETUM (CORNER) BERNICCHIA ET GORJÓN (BASIDIOMYCOTA) IN THE FAR EAST OF RUSSIA

2021 ◽  
Vol 69 ◽  
Author(s):  
N.V. Bukharova ◽  
Keyword(s):  
Nature Reserve ◽  
East Asian ◽  
Far East ◽  
Original Description ◽  
General Distribution ◽  
Far East Of Russia ◽  
Autonomous Region ◽  
The Far East ◽  
Far Eastern ◽  
First Time

Steccherinum aurantilaetum is a predominantly East Asian polyporoid fungus from the Steccherinaceae. It was first discovered in the Krasnoarmeisky District of the Primorye and in the Khabarovsk Territory. Previously, it was known only in the «Kedrovaya Pad» Nature Reserve in the Primorye and in the «Bastak» Nature Reserve in the Jewish Autonomous Region (for the territory of Russia). An original description of the species based on Far Eastern material is given, and a map of the general distribution of S. aurantilaetum is presented for the first time.

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