scholarly journals Development of a Portable Detection Method for Enteric Viruses from Ambient Air and Its Application to a Wastewater Treatment Plant

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 131 ◽  
Author(s):  
Koichi Matsubara ◽  
Hiroyuki Katayama

The ambient air from wastewater treatment plants has been considered as a potential source of pathogenic microorganisms to cause an occupational risk for the workers of the plants. Existing detection methods for enteric viruses from the air using a liquid as the collection medium therefore require special care to handle on-site. Knowledge accumulation on airborne virus risks from wastewater has been hindered by a lack of portable and handy collection methods. Enteric viruses are prevalent at high concentrations in wastewater; thus, the surrounding air may also be a potential source of viral transmission. We developed a portable collection and detection method for enteric viruses from ambient air and applied it to an actual wastewater treatment plant in Japan. Materials of the collection medium and eluting methods were optimized for real-time polymerase chain reaction-based virus quantification. The method uses a 4 L/min active air sampler, which is capable of testing 0.7–1.6 m3 air after 3–7 h sampling with a detection limit of 102 copies/m3 air in the field. Among 16 samples collected at five to seven locations in three sampling trials (November 2007–January 2008), 56% (9/16) samples were positive for norovirus (NV) GII, with the highest concentration of 3.2 × 103 copies/m3 air observed at the sampling point near a grit chamber. Adenoviruses (4/16), NV GI (6/16), FRNA bacteriophages GIII (3/16), and enteroviruses (3/16) were also detected but at lower concentrations. The virus concentration in the air was associated with that of the wastewater at each process. The results imply that the air from the sewer pipes or treatment process is contaminated by enteric viruses and thus special attention is needed to avoid accidental ingestion of viruses via air.

2019 ◽  
Vol 21 (1) ◽  
pp. 74-88 ◽  
Author(s):  
Fiona Wong ◽  
Matthew Robson ◽  
Lisa Melymuk ◽  
Chubashini Shunthirasingham ◽  
Nick Alexandrou ◽  
...  

Synthetic musk compounds found in ambient air and surface tributary waters may due to emissions from indoor air and release from wastewater treatment plant.


2018 ◽  
pp. 53-61
Author(s):  
Katarzyna Piekarz ◽  
Alicja Zawadzka

Odorous substances emitted to ambient air from wastewater treatment plants cause a serious nuisance to inhabitants in the direct vicinity of such emitters. The solutions currently used to remove malodorous gases in the Wastewater Treatment Plant in Belchatow do not fulfill their function properly. This article presents the test results of the composition and concentrations of odorous compounds emitted from the above- mentioned plant. In addition, the paper introduces the concept of eliminating substances by the biofiltration process. Due to the applied method, one can expect to reduce the odor by at least 90%.


2001 ◽  
Vol 43 (5) ◽  
pp. 223-229 ◽  
Author(s):  
K. ZAtasi ◽  
G. Fujita ◽  
G. Le Platte ◽  
C. Hufnagel ◽  
G. Keeler ◽  
...  

Specialized sampling equipment and ultra-clean analytical methodology were employed to quantify the concentrations or fluxes of mercury (Hg), cadmium (Cd) and polychlorinated biphenyl (PCBs) in ambient air, precipitation, runoff, sanitary sewer, and wastewater treatment plant (WWTP) influent. The relationship between the atmospheric deposition and runoff on controlled surfaces were explored for the three pollutants. The impact of the atmospheric deposition and runoff to the headwork loading of the WWTP were investigated. Atmospheric deposition was found to be the primary source of the mass of Cd, Hg, and PCBs in runoff from the controlled surfaces. Neither atmospheric deposition nor the runoff was the main sources of the three pollutants to the Detroit Wastewater Treatment Plant (WWTP). Wet weather flow contributes the main portion of the Cd, Hg, and PCBs loading to the WWTP.


1986 ◽  
Vol 18 (10) ◽  
pp. 47-52 ◽  
Author(s):  
A. Bosch ◽  
F. Lucena ◽  
J. Jofre

A wastewater treatment plant, which treats sewage by either mechanical primary sedimentation or lime coagulation, was used for modelling the fate of human enteric viruses after these treatments. The occurrence of human rotaviruses and enteroviruses was monitored in sewage influent, effluent and sludge samples. The level of indigenous enteric viruses in raw sewage was very high (up to 10 000 I.U./l); the values for rotavxrus and enterovxrus were of the same magnitude, although no correlation was found between the levels of both kinds of human enteric viruses. Both rotaviruses and enteroviruses were removed from sewage and transferred to sludge at similar rates after mechanical sedimentation, although rotaviruses were more efficiently recovered from sludge samples than enteroviruses. After chemical coagulation by lime treatment, most human enteric viruses in sludge were inactivated. Lime coagulation should be regarded as a most effective single-step treatment for the removal of viral pathogens such as rotaviruses and enteroviruses from sewage.


1996 ◽  
Vol 91 (3-4) ◽  
pp. 327-334 ◽  
Author(s):  
F. A. Aulicino ◽  
A. Mastrantonio ◽  
P. Orsini ◽  
C. Bellucci ◽  
M. Muscillo ◽  
...  

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