scholarly journals Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 266 ◽  
Author(s):  
Céline Deblanc ◽  
Aurélie Oger ◽  
Gaëlle Simon ◽  
Marie-Frédérique Le Potier
Keyword(s):  
Western Europe ◽  
Genotype I ◽  
Asian Origin ◽  
Domestic Pigs ◽  
Glycoprotein C ◽  
Wild Fauna ◽  
Hunting Dogs ◽  
Clade B ◽  
Herpesvirus 1 ◽  
Virus Strains

Pseudorabies (PR), also known as Aujeszky’s disease, is an economically important disease for the pig industry. It has been eradicated in domestic pigs in many European countries, including France, but its causative agent—Suid Herpesvirus 1—is still circulating in wild boars. The risk of endemic PR in wild fauna lies in reintroducing the virus among domestic pigs and transmitting it to other mammals, especially hunting dogs for which the disease is rapidly fatal. As such infections are regularly reported in France, this study genetically characterized canine PR virus strains in the country to obtain information on their diversity and evolution. Partial sequencing of the glycoprotein C-encoding gene from 55 virus strains isolated from dogs between 2006 and 2018 showed that 14 strains belonged to genotype I-clade A and another 38 to genotype I-clade B, two clades usually reported in Western Europe. More surprisingly, three strains were found to belong to genotype II, suggesting an Asian origin. Genotype I-clade A strains exhibited the highest diversity as five geographically segregated genogroups were identified.

Characterization of pseudorabies virus of wild boar origin from Europe

2010 ◽  
Vol 138 (11) ◽  
pp. 1590-1600 ◽  
Author(s):  
T. MÜLLER ◽  
B. G. KLUPP ◽  
C. FREULING ◽  
B. HOFFMANN ◽  
M. MOJCICZ ◽  
...  
Keyword(s):  
Wild Boar ◽  
Pseudorabies Virus ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Glycoprotein C ◽  
Hunting Dogs ◽  
Clade B ◽  
European Wild Boar ◽  
Causative Agents

SUMMARYPseudorabies virus (PrV) infections appear to be more widely distributed in the European wild boar (Sus scrofa) population than assumed. In Europe, attempts to isolate and characterize the causative agents have been limited so far. We therefore collected and examined a total of 35 PrV isolates obtained from wild boar or hunting dogs in Germany, France, Spain, Italy, Slovakia and Hungary between 1993 and 2008. Restriction enzyme analysis of genomic DNA using BamHI showed that all isolates, except one, belonged to genogroup I but different subtypes were evident. For further investigations of the phylogenetic relationships, a 732-bp fragment of the glycoprotein C (gC) gene was amplified by PCR. Sequence analysis revealed about 40 variant positions within this fragment. Comparison of the nucleotide sequences supported the separation into a clade containing isolates from North-Rhine Westphalia, Rhineland-Palatinate (Germany), France and Spain (clade B) and an apparently more variable clade comprising isolates from Brandenburg, Baden-Wurttemberg, Saxony, Saxony-Anhalt (Germany), Slovakia, Hungary, Italy and France (clade A).

Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

2001 ◽  
Vol 78 (4) ◽  
pp. 293-305 ◽  
Author(s):  
Praveen K. Gupta ◽  
Mohini Saini ◽  
L.K. Gupta ◽  
V.D.P. Rao ◽  
S.K. Bandyopadhyay ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Recombinant Bovine Herpesvirus-1 (BHV-1) Lacking Transactivator Protein BICPO Entails Lack of Glycoprotein C and Severely Reduced Infectivity

1996 ◽  
Vol 377 (12) ◽  
pp. 787-796 ◽  
Author(s):  
René Köppel ◽  
Cornel Fraefel ◽  
Bernd Vogt ◽  
Leonard J. Bello ◽  
William C. Lawrence ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1 ◽  
Transactivator Protein

scholarly journals Cloning and expression of bovine herpesvirus-1 glycoprotein C

IUBMB Life ◽  
1999 ◽  
Vol 47 (2) ◽  
pp. 275-282
Author(s):  
Praveen Gupta ◽  
Mohini Saini ◽  
L. K. Gupta ◽  
S. K. Bandyopadhyay ◽  
S. K. Garg
Keyword(s):  
Bovine Herpesvirus ◽  
Cloning And Expression ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Asian genotypes of dengue virus 4 in Brazil

2015 ◽  
Vol 143 (14) ◽  
pp. 3114-3117 ◽  
Author(s):  
A. C. O. PINHO ◽  
S. I. SARDI ◽  
F. L. PAULA ◽  
I. B. PEIXOTO ◽  
C. J. BRANDÃO ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Dengue Virus ◽  
Health Problem ◽  
Social Impact ◽  
Public Health Problem ◽  
Genotype I ◽  
Asian Origin ◽  
Northern Brazil ◽  
Dengue Virus Serotypes

SUMMARYDengue virus, commonly transmitted by mosquitoes, causes a human disease of significant social impact and presents a serious public health problem in Brazil. This report describes the unusual emergence of DENV-4 in northern Brazil after a nearly 30-year-long absence. DENV-4 genotype I is of Asian origin and was identified in the serum of patients receiving treatment at a hospital serving the Salvador area (Brazilian state of Bahia). The identification of dengue virus serotypes through molecular and phylogenetic analysis is essential for predicting disease severity or fatal illness, principally in endemic countries such as Brazil.

scholarly journals Epizootic process of demodicosis and sarcoptosis of pet dogs in the metropolis

2021 ◽  
pp. 140-146
Author(s):  
D. Feshchenko ◽  
O. Zghozinska ◽  
O. Dubova ◽  
T. Bakhur ◽  
T. Redko ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
Diagnostic Procedures ◽  
Sarcoptes Scabiei ◽  
Sarcoptic Mange ◽  
Domestic Dogs ◽  
Hunting Season ◽  
Veterinary Clinic ◽  
Demodex Canis ◽  
Wild Fauna ◽  
Hunting Dogs

Scabies mites, in particular Sarcoptes scabei and Demodex canis, remain undesirable but permanent members of zoocenoses in metropolis around the world. This is due to the contacts of domestic animals with wild relatives (foxes, raccoon dogs, coyotes), which are massively infected with various types of ticks. The purpose of our research was to find out the epizootic situation with demodicosis and sarcoptic mange of domestic dogs in Kyiv, as well as to establish the sources of their infection. The actual results were obtained on the basis of the "Veterinary Clinic of Doctor Medvedev" (Kyiv) according to the data of routine work with patients for 2009-2020. Diagnostic procedures consisted of clinical and dermatological examination of dogs, examination of skin scrapings, trichoscopy, "Scotch tape" test, a Wood's lamp examination and others. Over 11 years, 1473 dermatological examinations of domestic dogs were carried out. Of these, 230 dogs (15.6%) suffered from demodicosis at the age of 5 months to 7 years. Sarcoptic mange was much less common: a total of 59 sick dogs (4.0%) were identified. Both of acaroses were seasonal, with an increase in the incidence in fall and winter. Regarding the breed factor: Pugs (10.43%), Staffordshire Terriers (10.0%) and French Bulldogs (9.56%) were more prone to demodicosis; to sarcoptic mange – German wirehaired pointer (13.6%), German hunt terrier (11.9%) and Shorthaired pointer (10.2%). The stray dogs and cats were source most often of infection of domestic decorative dogs with mites Sarcoptes scabei var canis. Hunting dogs mainly became infected during the hunting season during contact with animals of the wild fauna, most often – foxes. Clinical manifestations of acaroses occurred mainly in young animals not older than 3 years (70.5 and 76.7% for demodicosis and sarcoptic mange, respectively). In addition, in male dogs sarcoptic mange is more often recorded (59.3%), in female dogs – demodicosis (53.9%). Key words: scabies, mites, Sarcoptes scabiei, Demodex canis, prevalence, dogs, foxes.

scholarly journals Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1910
Author(s):  
Jessica Navero-Castillejos ◽  
Rosa Benitez ◽  
Nuria Torner ◽  
José Muñoz ◽  
Daniel Camprubí-Ferrer ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Genotype I ◽  
Related Case ◽  
Geographical Regions ◽  
Surveillance Programs ◽  
Endemic Areas ◽  
Virus Strains

Dengue is the most significant arbovirus worldwide and a public health threat to non-endemic areas in which Aedes vectors are present. Autochthonous dengue transmission has been reported in several European countries in the last decade. Infected travelers from endemic regions arriving to areas colonized by Aedes albopictus in Europe need to be monitored in surveillance and control programs. We aimed to perform molecular characterization of RT-PCR-positive dengue cases detected in Catalonia, northeastern Spain, from 2013 to 2018. The basic demographic information and the geographical regions of importation were also analyzed. One-hundred four dengue cases were studied (103 imported infections and the first autochthonous case in our region). The dengue virus strains detected were serotyped and genotyped using molecular methods, and phylogenetic analyses were conducted. All four dengue serotypes were detected in travelers, including up to 10 different genotypes, reflecting the global circulation of dengue in endemic areas. The primary travel-related case of the 2018 autochthonous transmission was not identified, but the molecular analysis revealed dengue serotype 1, genotype I of Asian origin. Our results highlight the diversity of imported dengue virus strains and the role of molecular epidemiology in supporting arbovirus surveillance programs.

scholarly journals The impact of super-spreaders in COVID-19: mapping genome variation worldwide

2020 ◽  
Author(s):  
Alberto Gómez-Carballa ◽  
Xabier Bello ◽  
Jacobo Pardo-Seco ◽  
Federico Martinón-Torres ◽  
Antonio Salas
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Founder Effect ◽  
Human Pathogen ◽  
High Sequence Identity ◽  
Asian Origin ◽  
Sequence Identity ◽  
Public Repositories ◽  
The Impact ◽  
Bat Coronavirus ◽  
Virus Strains

AbstractThe human pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the major pandemic of the 21st century. We analyzed >4,700 SARS-CoV-2 genomes and associated meta-data retrieved from public repositories. SARS-CoV-2 sequences have a high sequence identity (>99.9%), which drops to >96% when compared to bat coronavirus. We built a mutation-annotated reference SARS-CoV-2 phylogeny with two main macro-haplogroups, A and B, both of Asian origin, and >160 sub-branches representing virus strains of variable geographical origins worldwide, revealing a uniform mutation occurrence along branches that could complicate the design of future vaccines. The root of SARS-CoV-2 genomes locates at the Chinese haplogroup B1, with a TMRCA dating to 12 November 2019 - thus matching epidemiological records. Sub-haplogroup A2a originates in China and represents the major non-Asian outbreak. Multiple founder effect episodes, most likely associated with super-spreader hosts, explain COVID-19 pandemic to a large extent.

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