scholarly journals CRISPR-cas3 of Salmonella Upregulates Bacterial Biofilm Formation and Virulence to Host Cells by Targeting Quorum-Sensing Systems

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 53 ◽  
Author(s):  
Luqing Cui ◽  
Xiangru Wang ◽  
Deyu Huang ◽  
Yue Zhao ◽  
Jiawei Feng ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Host Cells ◽  
Microbial Pathogens ◽  
Bacterial Invasion ◽  
Type I ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Sensing Systems ◽  
Type Three Secretion System

Salmonella is recognized as one of the most common microbial pathogens worldwide. The bacterium contains the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems, providing adaptive immunity against invading foreign nucleic acids. Previous studies suggested that certain bacteria employ the Cas proteins of CRISPR-Cas systems to target their own genes, which also alters the virulence during invasion of mammals. However, whether CRISPR-Cas systems in Salmonella have similar functions during bacterial invasion of host cells remains unknown. Here, we systematically analyzed the genes that are regulated by Cas3 in a type I-E CRISPR-Cas system and the virulence changes due to the deletion of cas3 in Salmonella enterica serovar Enteritidis. Compared to the cas3 gene wild-type (cas3 WT) Salmonella strain, cas3 deletion upregulated the lsrFGBE genes in lsr (luxS regulated) operon related to quorum sensing (QS) and downregulated biofilm-forming-related genes and Salmonella pathogenicity island 1 (SPI-1) genes related to the type three secretion system (T3SS). Consistently, the biofilm formation ability was downregulated in the cas3 deletion mutant (Δcas3). The bacterial invasive and intracellular capacity of Δcas3 to host cells was also reduced, thereby increasing the survival of infected host cells and live chickens. By the transcriptome-wide screen (RNA-Seq), we found that the cas3 gene impacts a series of genes related to QS, the flagellum, and SPI-1-T3SS system, thereby altering the virulence phenotypes. As QS SPI-1-T3SS and CRISPR-Cas systems are widely distributed in the bacteria kingdom, our findings extend our understanding of virulence regulation and pathogenicity in mammalian hosts for Salmonella and potentially other bacteria.

scholarly journals Selenium and tellurium-based nanoparticles as interfering factors in quorum sensing-regulated processes: violacein production and bacterial biofilm formation

Metallomics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1104-1114 ◽  
Author(s):  
Beatriz Gómez-Gómez ◽  
Lucia Arregui ◽  
Susana Serrano ◽  
Antonio Santos ◽  
Teresa Pérez-Corona ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Persistent Infections ◽  
Violacein Production ◽  
New Strategies

The effect of SeNPs and TeNPs on different processes regulated by QS such as violacein production and biofilm formation is presented. The data open new strategies for controlling persistent infections.

scholarly journals Temporal Quorum-Sensing Induction Regulates Vibrio cholerae Biofilm Architecture

2006 ◽  
Vol 75 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Zhi Liu ◽  
Fiona R. Stirling ◽  
Jun Zhu
Keyword(s):  
Quorum Sensing ◽  
Vibrio Cholerae ◽  
Biofilm Formation ◽  
High Cell Density ◽  
Intestinal Colonization ◽  
Signal Molecule ◽  
Biofilm Thickness ◽  
Sensing Systems ◽  
Biofilm Detachment ◽  
Biofilm Architecture

ABSTRACT Vibrio cholerae, the pathogen that causes cholera, also survives in aqueous reservoirs, probably in the form of biofilms. Quorum sensing negatively regulates V. cholerae biofilm formation through HapR, whose expression is induced at a high cell density. In this study, we show that the concentration of the quorum-sensing signal molecule CAI-1 is higher in biofilms than in planktonic cultures. By measuring hapR expression and activity, we found that the induction of quorum sensing in biofilm-associated cells occurs earlier. We further demonstrate that the timing of hapR expression is crucial for biofilm thickness, biofilm detachment rates, and intestinal colonization efficiency. These results suggest that V. cholerae is able to regulate its biofilm architecture by temporal induction of quorum-sensing systems.

scholarly journals Bicyclic brominated furanones: A new class of quorum sensing modulators that inhibit bacterial biofilm formation

2014 ◽  
Vol 22 (4) ◽  
pp. 1313-1317 ◽  
Author(s):  
Sijie Yang ◽  
Osama A. Abdel-Razek ◽  
Fei Cheng ◽  
Debjyoti Bandyopadhyay ◽  
Gauri S. Shetye ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
New Class ◽  
Brominated Furanones

scholarly journals Quorum-Sensing Systems in the Plant Growth-Promoting Bacterium Paraburkholderia phytofirmans PsJN Exhibit Cross-Regulation and Are Involved in Biofilm Formation

2017 ◽  
Vol 30 (7) ◽  
pp. 557-565 ◽  
Author(s):  
Ana Zúñiga ◽  
Raúl A. Donoso ◽  
Daniela Ruiz ◽  
Gonzalo A. Ruz ◽  
Bernardo González
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Type Strain ◽  
Wild Type Strain ◽  
Homoserine Lactone ◽  
Wild Type ◽  
Transcript Levels ◽  
Sensing Systems ◽  
Plant Growth Promoting ◽  
Growth Promoting

Quorum-sensing systems play important roles in host colonization and host establishment of Burkholderiales species. Beneficial Paraburkholderia species share a conserved quorum-sensing (QS) system, designated BraI/R, that controls different phenotypes. In this context, the plant growth-promoting bacterium Paraburkholderia phytofirmans PsJN possesses two different homoserine lactone QS systems BpI.1/R.1 and BpI.2/R.2 (BraI/R-like QS system). The BpI.1/R.1 QS system was previously reported to be important to colonize and produce beneficial effects in Arabidopsis thaliana plants. Here, we analyzed the temporal variations of the QS gene transcript levels in the wild-type strain colonizing plant roots. The gene expression patterns showed relevant differences in both QS systems compared with the wild-type strain in the unplanted control treatment. The gene expression data were used to reconstruct a regulatory network model of QS systems in P. phytofirmans PsJN, using a Boolean network model. Also, we examined the phenotypic traits and transcript levels of genes involved in QS systems, using P. phytofirmans mutants in homoserine lactone synthases genes. We observed that the BpI.1/R.1 QS system regulates biofilm formation production in strain PsJN and this phenotype was associated with the lower expression of a specific extracytoplasmic function sigma factor ecf26.1 gene (implicated in biofilm formation) in the bpI.1 mutant strain.

scholarly journals Mutation of luxS Affects Biofilm Formation in Streptococcus mutans

2003 ◽  
Vol 71 (4) ◽  
pp. 1972-1979 ◽  
Author(s):  
Justin Merritt ◽  
Fengxia Qi ◽  
Steven D. Goodman ◽  
Maxwell H. Anderson ◽  
Wenyuan Shi
Keyword(s):  
Quorum Sensing ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Microscopic Analysis ◽  
Homoserine Lactone ◽  
Good Evidence ◽  
Genome Project ◽  
Bacterial Biofilm ◽  
Wild Type

ABSTRACT Quorum sensing is a bacterial mechanism for regulating gene expression in response to changes in population density. Many bacteria are capable of acyl-homoserine lactone-based or peptide-based intraspecies quorum sensing and luxS-dependent interspecies quorum sensing. While there is good evidence about the involvement of intraspecies quorum sensing in bacterial biofilm, little is known about the role of luxS in biofilm formation. In this study, we report for the first time that luxS-dependent quorum sensing is involved in biofilm formation of Streptococcus mutans. S. mutans is a major cariogenic bacterium in the multispecies bacterial biofilm commonly known as dental plaque. An ortholog of luxS for S. mutans was identified using the data available in the S. mutans genome project (http://www.genome.ou.edu/smutans.html ). Using an assay developed for the detection of the LuxS-associated quorum sensing signal autoinducer 2 (AI-2), it was demonstrated that this ortholog was able to complement the luxS negative phenotype of Escherichia coli DH5α. It was also shown that AI-2 is indeed produced by S. mutans. AI-2 production is maximal during mid- to late-log growth in batch culture. Mutant strains devoid of the luxS gene were constructed and found to be defective in producing the AI-2 signal. There are also marked phenotypic differences between the wild type and the luxS mutants. Microscopic analysis of in vitro-grown biofilm structure revealed that the luxS mutant biofilms adopted a much more granular appearance, rather than the relatively smooth, confluent layer normally seen in the wild type. These results suggest that LuxS-dependent signal may play an important role in biofilm formation of S. mutans.

scholarly journals Two quorum sensing systems control biofilm formation and virulence in members of theBurkholderia cepaciacomplex

Virulence ◽  
2013 ◽  
Vol 4 (5) ◽  
pp. 400-409 ◽  
Author(s):  
Angela Suppiger ◽  
Nadine Schmid ◽  
Claudio Aguilar ◽  
Gabriella Pessi ◽  
Leo Eberl
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Sensing Systems ◽  
Systems Control

Disruption in Quorum-Sensing Systems and Bacterial Biofilm Inhibition by Cembranoid Diterpenes Isolated from the Octocoral Eunicea knighti

2012 ◽  
Vol 75 (9) ◽  
pp. 1637-1642 ◽  
Author(s):  
Edisson Tello ◽  
Leonardo Castellanos ◽  
Catalina Arévalo-Ferro ◽  
Carmenza Duque
Keyword(s):  
Quorum Sensing ◽  
Bacterial Biofilm ◽  
Biofilm Inhibition ◽  
Sensing Systems

scholarly journals Inhibition of Quorum Sensing in Pseudomonas aeruginosa by N-Acyl Cyclopentylamides

2007 ◽  
Vol 73 (10) ◽  
pp. 3183-3188 ◽  
Author(s):  
Takenori Ishida ◽  
Tsukasa Ikeda ◽  
Noboru Takiguchi ◽  
Akio Kuroda ◽  
Hisao Ohtake ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Homoserine Lactone ◽  
Ring Structure ◽  
Response Regulators ◽  
Maximal Inhibition ◽  
Sensing Systems ◽  
Reporter Assays

ABSTRACT N-Octanoyl cyclopentylamide (C8-CPA) was found to moderately inhibit quorum sensing in Pseudomonas aeruginosa PAO1. To obtain more powerful inhibitors, a series of structural analogs of C8-CPA were synthesized and examined for their ability to inhibit quorum sensing in P. aeruginosa PAO1. The lasB-lacZ and rhlA-lacZ reporter assays revealed that the chain length and the ring structure were critical for C8-CPA analogs to inhibit quorum sensing. N-Decanoyl cyclopentylamide (C10-CPA) was found to be the strongest inhibitor, and its concentrations required for half-maximal inhibition for lasB-lacZ and rhlA-lacZ expression were 80 and 90 μM, respectively. C10-CPA also inhibited production of virulence factors, including elastase, pyocyanin, and rhamnolipid, and biofilm formation without affecting growth of P. aeruginosa PAO1. C10-CPA inhibited induction of both lasI-lacZ by N-(3-oxododecanoyl)-l-homoserine lactone (PAI1) and rhlA-lacZ by N-butanoyl-l-homoserine lactone (PAI2) in the lasI rhlI mutant of P. aeruginosa PAO1, indicating that C10-CPA interferes with the las and rhl quorum-sensing systems via inhibiting interaction between their response regulators (LasR and RhlR) and autoinducers.

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