scholarly journals Mg-BGNs/DCECM Composite Scaffold for Cartilage Regeneration: A Preliminary In Vitro Study

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1550
Author(s):  
Zhiguo Yuan ◽  
Zhuocheng Lyu ◽  
Xin Liu ◽  
Jue Zhang ◽  
You Wang
Keyword(s):  
Tissue Engineering ◽  
Subchondral Bone ◽  
Composite Scaffold ◽  
In Vitro Study ◽  
Cartilage Regeneration ◽  
Osteochondral Lesions ◽  
Dual Function ◽  
Dependent Manner ◽  
Engineering Applications

Cartilage lesions can lead to progressive cartilage degeneration; moreover, they involve the subchondral bone, resulting in osteoarthritis (OA) onset and progression. Bioactive glasses, with the dual function of supporting both bone and cartilage regeneration, have become a promising biomaterial for cartilage/bone engineering applications. This is especially true for those containing therapeutic ions, which act as ion delivery systems and may further promote cartilage repair. In this study, we successfully fabricated Mg-containing bioactive glass nanospheres (Mg-BGNs) and constructed three different scaffolds, DCECM, Mg-BGNs-1/DCECM (1% Mg-BGNs), and Mg-BGNs-2/DCECM (10% Mg-BGNs) scaffold, by incorporating Mg-BGNs into decellularized cartilage extracellular matrix (DCECM). All three scaffolds showed favorable microarchitectural and ion controlled-release properties within the ideal range of pore size for tissue engineering applications. Furthermore, all scaffolds showed excellent biocompatibility and no signs of toxicity. Most importantly, the addition of Mg-BGNs to the DCECM scaffolds significantly promoted cell proliferation and enhanced chondrogenic differentiation induction of mesenchymal stem cells (MSCs) in pellet culture in a dose-dependent manner. Collectively, the multifunctional Mg-BGNs/DCECM composite scaffold not only demonstrated biocompatibility but also a significant chondrogenic response. Our study suggests that the Mg-BGNs/DCECM composite scaffold would be a promising tissue engineering tool for osteochondral lesions, with the ability to simultaneously stimulate articular cartilage and subchondral bone regeneration.

scholarly journals Cyclodextrin-Polypyrrole Coatings of Scaffolds for Tissue Engineering

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 459 ◽  
Author(s):  
Jan Lukášek ◽  
Šárka Hauzerová ◽  
Kristýna Havlíčková ◽  
Kateřina Strnadová ◽  
Karel Mašek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Conductive Polymers ◽  
Composite Scaffold ◽  
Cell Types ◽  
Hexanoic Acid ◽  
Spectroscopic Techniques ◽  
In Vitro Experiments ◽  
Engineering Applications ◽  
Different Cell Types

Polypyrrole is one of the most investigated conductive polymers used for tissue engineering applications because of its advantageous properties and the ability to promote different cell types’ adhesion and proliferation. Together with β-cyclodextrin, which is capable of accommodating helpful biomolecules in its cavity, it would make a perfect couple for use as a scaffold for tissue engineering. Such scaffolds were prepared by the polymerisation of 6-(pyrrol-3-yl)hexanoic acid on polycaprolactone microfibres with subsequent attachment of β-cyclodextrin on the polypyrrole layer. The materials were deeply characterised by several physical and spectroscopic techniques. Testing of the cyclodextrin enriched composite scaffold revealed its better performance in in vitro experiments compared with pristine polycaprolactone or polypyrrole covered polycaprolactone scaffolds.

scholarly journals In vitro study of novel microparticle based silk fibroin scaffold with osteoblast-like cells for load-bearing osteo-regenerative applications

RSC Advances ◽  
2017 ◽  
Vol 7 (43) ◽  
pp. 26551-26558 ◽  
Author(s):  
Nimisha Parekh ◽  
Chandni Hushye ◽  
Saniya Warunkar ◽  
Sayam Sen Gupta ◽  
Anuya Nisal
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
In Vitro Study ◽  
Vitro Study ◽  
Engineering Applications ◽  
Load Bearing ◽  
Silk Fibroin Scaffold

Silk Fibroin microparticle scaffolds show promise in bone tissue engineering applications.

In Vitro Osteogenic, Angiogenic, and Inflammatory Effects of Copper in β-Tricalcium Phosphate

MRS Advances ◽  
2019 ◽  
Vol 4 (21) ◽  
pp. 1253-1259
Author(s):  
Weiguo Han ◽  
Haley Cummings ◽  
Murali Krishna Duvuuru ◽  
Sarah Fleck ◽  
Sahar Vahabzadeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tricalcium Phosphate ◽  
Physical And Mechanical Properties ◽  
Early Time ◽  
Collagen Type I ◽  
Type I ◽  
Dependent Manner ◽  
Dental Tissue ◽  
Engineering Applications

AbstractTricalcium phosphate (TCP) is a promising candidate in bone and dental tissue engineering applications. Though osteoconductive, its low osteoinductivity is a major concern. Trace elements addition at low concentrations are known for their impact on not only the osteoinductivity, but also physical and mechanical properties of TCP. Copper (Cu) is known for its role in vascularization and angiogenesis in biological systems. Here, we studied the effects of Cu addition on phase composition, porosity, microstructure and in vitro interaction with osteoblast (OB) cells. Our results showed that Cu stabilized the TCP structure, while no significant effect of microstructure and porosity was found. Cu at concentrations less than 1 wt.% did not have any cytotoxic effect while decreased proliferation of OBs were observed at 1 wt.% Cu doped TCP. Addition of Cu upregulated collagen type I and vascular endothelial growth factor expression in a dose dependent manner at early time-point. Furthermore, Cu reduced inflammatory gene expression by human osteoblasts. These findings show that addition of Cu to TCP may provide a therapeutic strategy that can be applied in bone tissue engineering applications.

scholarly journals In vitro study of directly bioprinted perfusable vasculature conduits

2015 ◽  
Vol 3 (1) ◽  
pp. 134-143 ◽  
Author(s):  
Yahui Zhang ◽  
Yin Yu ◽  
Adil Akkouch ◽  
Amer Dababneh ◽  
Farzaneh Dolati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vitro Study ◽  
Vitro Study ◽  
Engineering Applications

This paper highlight characterization of directly bioprinted perfusable vascular conduits for tissue engineering applications.

Bio-Hybrid Composite Scaffold from Silk Fibroin/Chitosan/Mesoporous Bioactive Glass Microspheres for Tissue Engineering Applications

2015 ◽  
Vol 1131 ◽  
pp. 79-83 ◽  
Author(s):  
Ratiya Phetnin ◽  
Sirirat Tubsungnoen Rattanachan
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Silk Fibroin ◽  
Hybrid Composite ◽  
Composite Scaffold ◽  
Composite Scaffolds ◽  
Bioactive Glasses ◽  
Engineering Applications ◽  
Mesoporous Bioactive Glass

This research aims to fabricate the novel bio-hybrid composite scaffold from mesoporous bioactive glasses/silk fibroin/chitosan (MBGs/SF/CS) for use in tissue engineering applications. MBGs/SF/CS composite scaffolds were successfully fabricated using freezing and lyophilization process. Two types of mesoporous bioactive glasses which were irregular and spherical shape were dispersed in the silk fibroin/chitosan based scaffolds in order to improve the mechanical strength and bioactivity. SEM observation showed the interconnected pores with pore size from 100 to 300 µm. XRD and FTIR exhibited the present of silk fibroin, chitosan, and MBGs in composite scaffolds. The incorporation of MBGs in SF/CS scaffolds significantly increased the compressive strength of scaffolds. The composite scaffolds were immersed in the simulated body fluid (SBF) for in vitro bioactivity test. The in vitro bioactivity results indicated that the MBGs/SF/CS induced hydroxycarbonate apatite (HCA) formation while there was no change for SF/CS scaffolds. Furthermore, mesoporous bioactive glass with micro-spherical particles (MBGMs) which easily dispersed in SF/CS solution during the fabrication of scaffolds as compared to mesoporous bioactive glass with irregular shape (MBGs). The results showed that MBGs/SF/CS composite scaffolds could be useful composite scaffolds for tissue engineering applications.

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