scholarly journals Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 589
Author(s):  
Fionna M. D. Samuels ◽  
Dominik G. Stich ◽  
Remi Bonnart ◽  
Gayle M. Volk ◽  
Nancy E. Levinger

Cryoprotectants allow cells to be frozen in liquid nitrogen and cryopreserved for years by minimizing the damage that occurs in cooling and warming processes. Unfortunately, how the specific cryoprotectants keep the cells viable through the cryopreservation process is not entirely evident. This contributes to the arduous process of optimizing cryoprotectant formulations for each new cell line or species that is conserved. Coherent anti-Stokes Raman scattering microscopy facilitates the visualization of deuterated cryoprotectants within living cells. Using this technique, we directly imaged the location of fully deuterated dimethyl sulfoxide (d6-DMSO), the deuterated form of a commonly used cryoprotectant, DMSO, within rice suspension cells. This work showed that d6-DMSO does not uniformly distribute throughout the cells, rather it enters the cell and sequesters within organelles, changing our understanding of how DMSO concentration varies within the cellular compartments. Variations in cryoprotectant concentration within different cells and tissues will likely lead to differing protection from liquid nitrogen exposure. Expanding this work to include different cryoprotectants and mixtures of cryoprotectants is vital to create a robust understanding of how the distributions of these molecules change when different cryoprotectants are used.

2017 ◽  
Vol 19 (21) ◽  
pp. 13438-13442 ◽  
Author(s):  
Ryo Furuta ◽  
Naoyuki Kurake ◽  
Kenji Ishikawa ◽  
Keigo Takeda ◽  
Hiroshi Hashizume ◽  
...  

Interactions between non-equilibrium atmospheric-pressure plasma (NEAPP) and living cells were examined using multiplex coherent anti-Stokes Raman scattering (CARS) microscopy.


2017 ◽  
Vol 523 (1) ◽  
pp. 270-280 ◽  
Author(s):  
Jukka Saarinen ◽  
Erkan Sözeri ◽  
Sara J. Fraser-Miller ◽  
Leena Peltonen ◽  
Hélder A. Santos ◽  
...  

2009 ◽  
Vol 17 (12) ◽  
pp. 9526 ◽  
Author(s):  
Takeo Minamikawa ◽  
Mamoru Hashimoto ◽  
Katsumasa Fujita ◽  
Satoshi Kawata ◽  
Tsutomu Araki

ChemPhysChem ◽  
2018 ◽  
Vol 19 (9) ◽  
pp. 1048-1055 ◽  
Author(s):  
Fisseha Bekele Legesse ◽  
Jan Rüger ◽  
Tobias Meyer ◽  
Christoph Krafft ◽  
Michael Schmitt ◽  
...  

2006 ◽  
Vol 31 (12) ◽  
pp. 1872 ◽  
Author(s):  
Feruz Ganikhanov ◽  
Conor L. Evans ◽  
Brian G. Saar ◽  
X. Sunney Xie

2017 ◽  
Vol 22 (7) ◽  
pp. 070502 ◽  
Author(s):  
Sixian You ◽  
Yuan Liu ◽  
Zane Arp ◽  
Youbo Zhao ◽  
Eric J. Chaney ◽  
...  

2020 ◽  
Vol 74 (7) ◽  
pp. 751-757
Author(s):  
Wallance M. Pazin ◽  
Leonardo N. Furini ◽  
Vita Solovyeva ◽  
Tibebe Lemma ◽  
Rafael J. G. Rubira ◽  
...  

In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm−1, assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action.


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