scholarly journals Genetic and Molecular Control of Somatic Embryogenesis

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1467
Author(s):  
Camille Salaün ◽  
Loïc Lepiniec ◽  
Bertrand Dubreucq
Keyword(s):  
Somatic Embryogenesis ◽  
Transcription Factors ◽  
Abscisic Acid ◽  
Plant Species ◽  
Asexual Reproduction ◽  
Clonal Propagation ◽  
Zygotic Embryogenesis ◽  
Molecular Control ◽  
Master Regulators ◽  
Precise Role

Somatic embryogenesis is a method of asexual reproduction that can occur naturally in various plant species and is widely used for clonal propagation, transformation and regeneration of different crops. Somatic embryogenesis shares some developmental and physiological similarities with zygotic embryogenesis as it involves common actors of hormonal, transcriptional, developmental and epigenetic controls. Here, we provide an overview of the main signaling pathways involved in the induction and regulation of somatic embryogenesis with a focus on the master regulators of seed development, LEAFY COTYLEDON 1 and 2, ABSCISIC ACID INSENSITIVE 3 and FUSCA 3 transcription factors whose precise role during both zygotic and somatic embryogenesis remains to be fully elucidated.

scholarly journals Evaluation of induction of somatic embryogenesis from cotyledonary leaves of Banana Passion fruit (Passiflora mollissima) L.H Bailey

Respuestas ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 31-38
Author(s):  
Oscar José Parra Peñalosa ◽  
Giovanni Orlando Cancino Escalante
Keyword(s):  
Somatic Embryogenesis ◽  
South America ◽  
Genetic Transformation ◽  
High Resistance ◽  
Culture Medium ◽  
Clonal Propagation ◽  
Zygotic Embryogenesis ◽  
Commercial Use ◽  
The Andes ◽  
Embryo Formation

Passiflora mollissima  L.H Bailey is an endemic species from the Andes mountain of Colombia in South America with important edible fruits and medicinal and economical properties. The development of improved micropropagation techniques is necessary to provide rapid and efficient clonal propagation of elite genotypes with high resistance and uniform production, as well as a system that can be used for genetic transformation. For this reason, the investigation focused on the evaluation of induction of somatic embryogenesis in P. mollissima from cotyledonary leaves, the effect of growth regulator concentrations and the orientation of explants on embryo production. Histological analyses of somatic embryogenesis were performed every 10 days after induction over 38 days of exposure to the medium. Results showed somatic embryo formation on Murashige and Skoog, (1962) culture medium supplemented with 4.5 μM 2,4-diclorophenoxyacetic acid plus 4.5 μM 6-benzyladenine. The results obtained are applicable to knowledge of non-zygotic embryogenesis in passionflower of the Andean region, for the purpose of improvement and commercial use. It is noteworthy that this is the first study in the induction and obtaining of embryos in P. mollissima.

scholarly journals Genetic activity during early plant embryogenesis

2020 ◽  
Vol 477 (19) ◽  
pp. 3743-3767
Author(s):  
Ran Tian ◽  
Priyanka Paul ◽  
Sanjay Joshi ◽  
Sharyn E. Perry
Keyword(s):  
Somatic Embryogenesis ◽  
Transcription Factors ◽  
Somatic Embryo ◽  
Zygotic Embryo ◽  
Zygotic Embryogenesis ◽  
Regeneration Strategy ◽  
Molecular Events ◽  
Genetic Activity ◽  
Test Gene ◽  
Plant Embryogenesis

Seeds are essential for human civilization, so understanding the molecular events underpinning seed development and the zygotic embryo it contains is important. In addition, the approach of somatic embryogenesis is a critical propagation and regeneration strategy to increase desirable genotypes, to develop new genetically modified plants to meet agricultural challenges, and at a basic science level, to test gene function. We briefly review some of the transcription factors (TFs) involved in establishing primary and apical meristems during zygotic embryogenesis, as well as TFs necessary and/or sufficient to drive somatic embryo programs. We focus on the model plant Arabidopsis for which many tools are available, and review as well as speculate about comparisons and contrasts between zygotic and somatic embryo processes.

Abscisic acid-responsive element binding transcription factors contribute to proline synthesis and stress adaptation in Arabidopsis

2021 ◽  
Vol 261 ◽  
pp. 153414
Author(s):  
Asis Shrestha ◽  
Daniel Kingsley Cudjoe ◽  
Mohammad Kamruzzaman ◽  
Shahid Siddique ◽  
Fabio Fiorani ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abscisic Acid ◽  
Stress Adaptation ◽  
Abscisic Acid Responsive Element ◽  
Responsive Element

Involvement of WRKY Transcription Factors in Abscisic-Acid-Induced Cold Tolerance of Banana Fruit

2017 ◽  
Vol 65 (18) ◽  
pp. 3627-3635 ◽  
Author(s):  
Dong-lan Luo ◽  
Liang-jie Ba ◽  
Wei Shan ◽  
Jian-fei Kuang ◽  
Wang-jin Lu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abscisic Acid ◽  
Cold Tolerance ◽  
Banana Fruit ◽  
Wrky Transcription Factors

Initiation and morphological development of somatic embryoids from barley cell cultures

1984 ◽  
Vol 62 (6) ◽  
pp. 1245-1249 ◽  
Author(s):  
L. S. Kott ◽  
K. J. Kasha
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Cell Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Morphological Development ◽  
Low Concentrations ◽  
The Media ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Barley Cell

Somatic embryogenesis was induced in callus previously initiated from immature embryos of barley. These cultures ranged in age from 6 weeks to 30 months. Embryoids were readily initiated from homogenized suspension-grown aggregates when plated on modified B5 media with 2,4-dichlorophenoxyacetic acid. Low concentrations (0.1 and 0.05 mg∙L−1) of abscisic acid promoted further maturation of embryoids, while gibberellic acid (1 mg∙L−1) and kinetin (0.1 mg∙L−1) were used in the media to encourage embryoid germination. The development of somatic embryoids from initiation through maturation and germination is described.

scholarly journals The Arabidopsis Mediator Subunit MED25 Differentially Regulates Jasmonate and Abscisic Acid Signaling through Interacting with the MYC2 and ABI5 Transcription Factors

2012 ◽  
Vol 24 (7) ◽  
pp. 2898-2916 ◽  
Author(s):  
Rong Chen ◽  
Hongling Jiang ◽  
Lin Li ◽  
Qingzhe Zhai ◽  
Linlin Qi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abscisic Acid ◽  
Abscisic Acid Signaling

scholarly journals Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Somatic Embryos ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Direct Somatic Embryogenesis ◽  
Fresh Weight ◽  
High Concentration ◽  
Globular Stage ◽  
Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

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