scholarly journals Quinoa (Chenopodium quinoa Willd.): Genetic Diversity According to ISSR and SCoT Markers, Relative Gene Expression, and Morpho-Physiological Variation under Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2802
Author(s):  
Diaa Abd El-Moneim ◽  
Eman I. S. ELsarag ◽  
Salman Aloufi ◽  
Asmaa M. El-Azraq ◽  
Salha Mesfer ALshamrani ◽  
...  

Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic stresses, including salt. The present research examined the mechanisms of salt tolerance in five different quinoa genotypes at four different salinity levels (control (60), 80, 120, and 160 mM NaCl). ISSR and SCoT analysis revealed high polymorphism percentages of 90.91% and 85.26%, respectively. Furthermore, ISSR 1 and SCoT 7 attained the greatest number of polymorphic amplicons (27 and 26), respectively. Notably, LINE-6 and M-28 genotypes demonstrated the greatest number of unique positive and negative amplicons (50 and 42) generated from ISSR and SCoT, respectively. Protein pattern analysis detected 11 bands with a polymorphism percentage 27.27% among the quinoa genotypes, with three unique bands distinguishable for the M-28 genotype. Similarity correlation indicated that the highest similarity was between S-10 and Regeolone-3 (0.657), while the lowest similarity was between M-28 and LINE-6 (0.44). Significant variations existed among the studied salinity treatments, genotypes, and the interactions between them. The highest and lowest values for all the studied morpho-physiological and biochemical traits were recorded at 60 and 160 mM NaCl concentrations, respectively, except for the Na and proline contents, which exhibited the opposite relationship. The M-28 genotype demonstrated the highest values for all studied characteristics, while the LINE-6 genotype represented the lowest in both seasons. On the other hand, mRNA transcript levels for CqSOS1 did not exhibit differential expression in roots and leaf tissues, while the expression of CqNHX1 was upregulated more in both tissues for the M-28 genotype than for the LINE-6 genotype, and its maximum induction was seen in the leaves. Overall, the genotypes M-28 and LINE-6 were identified as the most and least salinity-tolerant, respectively.

2021 ◽  
Vol 168 ◽  
pp. 113595
Author(s):  
Marcos Vinicius Silva de Andrade ◽  
Renato Delmondez de Castro ◽  
Diego da Silva Cunha ◽  
Valdir Gomes Neto ◽  
Maria Gabriela Aparecida Carosio ◽  
...  

Aquaculture ◽  
2017 ◽  
Vol 467 ◽  
pp. 138-148 ◽  
Author(s):  
Fiddy S. Prasetiya ◽  
Luc A. Comeau ◽  
Romain Gastineau ◽  
Priscilla Decottignies ◽  
Bruno Cognie ◽  
...  

2020 ◽  
Vol 8 (5) ◽  
pp. 2277-2281
Author(s):  
KN Ravitej ◽  
P Ratna Kumar ◽  
S Narender Reddy ◽  
Praduman Yadav V ◽  
Gouri Shankar ◽  
...  

Author(s):  
Amrita Kumari ◽  
R. D. Ranjan ◽  
Chandan Roy ◽  
Awadesh Kumar Pal ◽  
S. Kumar

Heat stress, particularly the stress appears at the time of flowering to grain filling stages causing severe yield loss in wheat. Heat tolerance is complex phenomena that include adjustment in morphological, physiological and biochemical traits of the crop. Present investigation was carried out to understand the effect of terminal heat stress on different traits of wheat. The experiment was conducted in three dates of sowing as timely sown, late sown and very late sown to expose the crop to heat stress at later stages of the crop growth. Significant genetic variations for all the traits evaluated under three conditions indicated the presence of variability for the traits. Trait association analysis revealed that flag leaf chlorophyll content and MSI at seedling stage; MDA at reproductive stage had direct relationship with grain yield. While under very late sown condition MDA and RWC at seedling stages were found to be highly correlated with grain yield. It indicates that MDA, RWC at seedling stage and days to booting, days to milking plays important role in very late sown condition that can be used as selection criteria in breeding programme.


2021 ◽  
Author(s):  
Shayla Bindra ◽  
Inderjit Singh ◽  
Satinder Singh ◽  
Ashutosh Kushwah ◽  
B. S. Gill ◽  
...  

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