scholarly journals bHLH Transcription Factor NtMYC2a Regulates Carbohydrate Metabolism during the Pollen Development of Tobacco (Nicotiana tabacum L. cv. TN90)

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 17
Author(s):  
Shiquan Bian ◽  
Tian Tian ◽  
Yongqiang Ding ◽  
Ning Yan ◽  
Chunkai Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Carbohydrate Metabolism ◽  
Pollen Development ◽  
Starch Accumulation ◽  
Bhlh Transcription Factor ◽  
Pollen Maturation ◽  
Nicotiana Tabacum L

Basic helix-loop-helix (bHLH) transcription factor MYC2 regulates plant growth and development in many aspects through the jasmonic acid (JA) signaling pathway, while the role of MYC2 in plant carbohydrate metabolism has not been reported. Here, we generated NtMYC2a-overexpressing (NtMYC2a-OE) and RNA-interference-mediated knockdown (NtMYC2a-RI) transgenic plants of tobacco (Nicotiana tabacum L. cv. TN90) to investigate the role of NtMYC2a in carbohydrate metabolism and pollen development. Results showed that NtMYC2a regulates the starch accumulation and the starch-sugar conversion of floral organs, especially in pollen. The RT-qPCR analysis showed that the expression of starch-metabolic-related genes, AGPs, SS2 and BAM1, were regulated by NtMYC2a in the pollen grain, anther wall and ovary of tobacco plants. The process of pollen maturation was accelerated in NtMYC2a-OE plants and was delayed in NtMYC2a-RI plants, but the manipulation of NtMYC2a expression did not abolish the pollen fertility of the transgenic plants. Intriguingly, overexpression of NtMYC2a also enhanced the soluble carbohydrate accumulation in tobacco ovaries. Overall, our results demonstrated that the bHLH transcription factor NtMYC2a plays an important role in regulating the carbohydrate metabolism during pollen maturation in tobacco.

scholarly journals Increased Leaf Nicotine Content by Targeting Transcription Factor Gene Expression in Commercial Flue-Cured Tobacco (Nicotiana tabacum L.)

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 930 ◽  
Author(s):  
Hai Liu ◽  
Tatyana I. Kotova ◽  
Michael P. Timko
Keyword(s):  
Transcription Factor ◽  
Nicotiana Tabacum ◽  
Gene Promoter ◽  
Bhlh Transcription Factor ◽  
Helix Loop Helix ◽  
Nicotiana Tabacum L ◽  
Meja Treatment ◽  
Transgenic Lines ◽  
Nicotine Biosynthesis ◽  
High Level

Nicotine, the most abundant pyridine alkaloid in cultivated tobacco (Nicotiana tabacum L.), is a potent inhibitor of insect and animal herbivory and a neurostimulator of human brain function. Nicotine biosynthesis is controlled developmentally and can be induced by abiotic and biotic stressors via a jasmonic acid (JA)-mediated signal transduction mechanism involving members of the APETALA 2/ethylene-responsive factor (AP2/ERF) and basic helix-loop-helix (bHLH) transcription factor (TF) families. AP2/ERF and bHLH TFs work combinatorically to control nicotine biosynthesis and its subsequent accumulation in tobacco leaves. Here, we demonstrate that overexpression of the tobacco NtERF32, NtERF221/ORC1, and NtMYC2a TFs leads to significant increases in nicotine accumulation in T2 transgenic K326 tobacco plants before topping. Up to 9-fold higher nicotine production was achieved in transgenics overexpressing NtERF221/ORC1 under the control of a constitutive GmUBI3 gene promoter compared to wild-type plants. The constitutive 2XCaMV35S promoter and a novel JA-inducible 4XGAG promoter were less effective in driving high-level nicotine formation. Methyljasmonic acid (MeJA) treatment further elevated nicotine production in all transgenic lines. Our results show that targeted manipulation of NtERF221/ORC1 is an effective strategy for elevating leaf nicotine levels in commercial tobacco for use in the preparation of reduced risk tobacco products for smoking replacement therapeutics.

A novel bHLH transcription factor, NtbHLH1, modulates iron homeostasis in tobacco (Nicotiana tabacum L.)

2020 ◽  
Vol 522 (1) ◽  
pp. 233-239
Author(s):  
Yang-Yang Li ◽  
Xiao-Yan Sui ◽  
Jia-Shuo Yang ◽  
Xiao-Hua Xiang ◽  
Zun-Qiang Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nicotiana Tabacum ◽  
Iron Homeostasis ◽  
Bhlh Transcription Factor ◽  
Nicotiana Tabacum L

Goosecoid suppresses cell growth and enhances neuronal differentiation of PC12 cells

2000 ◽  
Vol 113 (15) ◽  
pp. 2705-2713
Author(s):  
K. Sawada ◽  
Y. Konishi ◽  
M. Tominaga ◽  
Y. Watanabe ◽  
J. Hirano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neurite Outgrowth ◽  
Neuronal Differentiation ◽  
Pc12 Cells ◽  
Mammalian Cells ◽  
Homeobox Gene ◽  
S Phase ◽  
Bhlh Transcription Factor ◽  
Spemann Organizer

In all vertebrate species, the homeobox gene goosecoid serves as a marker of the Spemann organizer tissue. One function of the organizer is the induction of neural tissue. To investigate the role of goosecoid in neuronal differentiation of mammalian cells, we have introduced goosecoid into PC12 cells. Expression of goosecoid resulted in reduced cell proliferation and enhanced neurite outgrowth in response to NGF. Expression of goosecoid led to a decrease in the percentage of S-phase cells and to upregulation of the expression of the neuron-specific markers MAP-1b and neurofilament-L. Analysis of goosecoid mutants revealed that these effects were independent of either DNA binding or homodimerization of Goosecoid. Coexpression of the N-terminal portion of the ets transcription factor PU.1, a protein that can bind to Goosecoid, repressed neurite outgrowth and rescued the proliferation of PC12 cultures. In contrast, expression of the bHLH transcription factor HES-1 repressed goosecoid-mediated neurite outgrowth without changing the proportion of S-phase cells. These results suggest that goosecoid is involved in neuronal differentiation in two ways, by slowing the cell cycle and stimulating neurite outgrowth, and that these two events are separately regulated.

The Possible Role of Zearalenone in the Floral Gradient in Nicotiana tabacum L.

1995 ◽  
Vol 147 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Yongfu Fu ◽  
Hongying Li ◽  
Fanjing Meng
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

Induced Accumulation and Potential Antioxidative Function of Rutin in Two Cultivars of Nicotiana tabacum L.

1994 ◽  
Vol 49 (1-2) ◽  
pp. 57-62 ◽  
Author(s):  
Thomas Steger-Hartmann ◽  
Ulrich Koch ◽  
Thomas Dunz ◽  
Edgar Wagner
Keyword(s):  
Oxidative Stress ◽  
Nicotiana Tabacum ◽  
Potential Role ◽  
Ion Leakage ◽  
The Sun ◽  
Antioxidative Function ◽  
Ozone Sensitivity ◽  
Nicotiana Tabacum L ◽  
Tert Butylhydroperoxide

The rutin (quercetin-3-rhamnosyl-glucoside) content of two tobacco cultivars (Nicotiana tabacum L.) which differ in their ozone-sensitivity was assayed after exposure to various rutininducing stimuli. In the growth-chamber, UV radiation in combination with white light led to the accumulation of similar amounts of rutin in both cultivars. Treatment with radical producing agents (tert-butylhydroperoxide and paraquat) also led to rutin accumulation. In this case, the rutin content was higher in the tolerant cultivar. The rutin content was also higher in the tolerant cultivar upon exposure of the plants on an out-door stand, even when the UV-part of the sun spectrum was excluded by cut-off filters. The potential role of rutin as antioxidant was tested with an ion leakage assay. Plants with relatively high rutin content were less sensitive towards paraquat-induced ion leakage than plants without rutin. Thus, the higher rutin content of the ozone-tolerant cultivar Bel B may well contribute to its tolerance against oxidative stress.

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Planta ◽  
2006 ◽  
Vol 225 (2) ◽  
pp. 341-351 ◽  
Author(s):  
Chinnathambi Srinivasan ◽  
Zongrang Liu ◽  
Iris Heidmann ◽  
Ence Darmo Jaya Supena ◽  
Hiro Fukuoka ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nicotiana Tabacum ◽  
Heterologous Expression ◽  
Baby Boom ◽  
Regeneration Capacity ◽  
Nicotiana Tabacum L ◽  
Erf Transcription Factor

Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of SnRK2.8 in the drought-stress response

2012 ◽  
Vol 448 (3) ◽  
pp. 353-363 ◽  
Author(s):  
Mi Jung Kim ◽  
Mi-Jeong Park ◽  
Pil Joon Seo ◽  
Jin-Su Song ◽  
Hie-Joon Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Drought Resistance ◽  
Nuclear Import ◽  
Proteolytic Processing ◽  
Transcriptional Responses ◽  
Resistance Response ◽  
Proteolytic Activation

Controlled proteolytic activation of membrane-anchored transcription factors provides an adaptation strategy that guarantees rapid transcriptional responses to abrupt environmental stresses in both animals and plants. NTL6 is a plant-specific NAC [NAM/ATAF1/2/CUC2] transcription factor that is expressed as a dormant plasma membrane-associated form in Arabidopsis. Proteolytic processing of NTL6 is triggered by abiotic stresses and ABA (abscisic acid). In the present study, we show that NTL6 is linked directly with SnRK (Snf1-related protein kinase) 2.8-mediated signalling in inducing a drought-resistance response. SnRK2.8 phosphorylates NTL6 primarily at Thr142. NTL6 phosphorylation by SnRK2.8 is required for its nuclear import. Accordingly, a mutant NTL6 protein, in which Thr142 was mutated to an alanine, was poorly phosphorylated and failed to enter the nucleus. In accordance with the role of SnRK2.8 in drought-stress signalling, transgenic plants overproducing either NTL6 or its active form 6ΔC (35S:NTL6 and 35S:6ΔC) exhibited enhanced resistance to water-deficit conditions such as those overproducing SnRK2.8 (35S:SnRK2.8). In contrast, NTL6 RNAi (RNA interference) plants were susceptible to dehydration as observed in the SnRK2.8-deficient snrk2.8-1 mutant. Furthermore, the dehydration-resistant phenotype of 35S:NTL6 transgenic plants was compromised in 35S:NTL6 X snrk2.8-1 plants. These observations indicate that SnRK2.8-mediated protein phosphorylation, in addition to a proteolytic processing event, is important for NTL6 function in inducing a drought-resistance response.

scholarly journals Merkel Cell Polyomavirus T Antigens Induce Merkel Cell-Like Differentiation in GLI1-Expressing Epithelial Cells

Cancers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1989
Author(s):  
Thibault Kervarrec ◽  
Mahtab Samimi ◽  
Sonja Hesbacher ◽  
Patricia Berthon ◽  
Marion Wobser ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Human Keratinocytes ◽  
Merkel Cell Polyomavirus ◽  
Bhlh Transcription Factor ◽  
Merkel Cell ◽  
T Antigens ◽  
Differentiation Potential ◽  
Transcription Factor 1

Merkel cell carcinoma (MCC) is an aggressive skin cancer frequently caused by the Merkel cell polyomavirus (MCPyV). It is still under discussion, in which cells viral integration and MCC development occurs. Recently, we demonstrated that a virus-positive MCC derived from a trichoblastoma, an epithelial neoplasia bearing Merkel cell (MC) differentiation potential. Accordingly, we hypothesized that MC progenitors may represent an origin of MCPyV-positive MCC. To sustain this hypothesis, phenotypic comparison of trichoblastomas and physiologic human MC progenitors was conducted revealing GLI family zinc finger 1 (GLI1), Keratin 17 (KRT 17), and SRY-box transcription factor 9 (SOX9) expressions in both subsets. Furthermore, GLI1 expression in keratinocytes induced transcription of the MC marker SOX2 supporting a role of GLI1 in human MC differentiation. To assess a possible contribution of the MCPyV T antigens (TA) to the development of an MC-like phenotype, human keratinocytes were transduced with TA. While this led only to induction of KRT8, an early MC marker, combined GLI1 and TA expression gave rise to a more advanced MC phenotype with SOX2, KRT8, and KRT20 expression. Finally, we demonstrated MCPyV-large T antigens’ capacity to inhibit the degradation of the MC master regulator Atonal bHLH transcription factor 1 (ATOH1). In conclusion, our report suggests that MCPyV TA contribute to the acquisition of an MC-like phenotype in epithelial cells.

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