Ectopic Expression of the Homeotic MADS-Box Gene HAM31 (Helianthus annuus L.) in Transgenic Plants Nicotiana tabacum L. Affects the Gynoecium Identity

2018 ◽  
Vol 483 (1) ◽  
pp. 363-368
Author(s):  
A. V. Shchennikova ◽  
O. A. Shulga ◽  
K. G. Skryabin
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Helianthus Annuus ◽  
Ectopic Expression ◽  
Mads Box ◽  
Helianthus Annuus L ◽  
Nicotiana Tabacum L ◽  
Mads Box Gene

scholarly journals Genome-Wide Identification, Gene Structure and Expression Analysis of the MADS-Box Gene Family Indicate Their Function in the Development of Tobacco (Nicotiana tabacum L.)

2019 ◽  
Vol 20 (20) ◽  
pp. 5043 ◽  
Author(s):  
Bai ◽  
Yang ◽  
Cao ◽  
Yao ◽  
Zhang ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Expression Profiles ◽  
Mads Box ◽  
In Planta ◽  
Genome Wide ◽  
Nicotiana Tabacum L ◽  
Tobacco Genome ◽  
Mads Box Gene ◽  
Gene Structures ◽  
Gene Structure And Expression

MADS-box genes play a pivotal role in various processes, including floral and seed development, controlling flowering time, regulation of fruits ripening, and respond to abiotic and biotic stressors in planta. Tobacco (Nicotiana tabacum) has been widely used as a model plant for analyzing the gene function, however, there has been less information on the regulation of flowering, and the associated genes. In the present study, a total of 168 NtMADS-box genes were identified from tobacco, and their phylogenetic relationship, chromosome locations, and gene structures were further analyzed. NtMADS-box genes can be clustered into four sub-families of Mα, Mγ, MIKC*, and MIKCC. A total of 111 NtMADS-box genes were distributed on 20 chromosomes, and 57 NtMADS-box genes were located on the unanchored scaffolds due to the complex and incomplete assembly of the tobacco genome. Expression profiles of NtMADS-box genes by microarray from 23 different tissues indicated that members in different NtMADS-box gene subfamilies might play specific roles in the growth and flower development, and the transcript levels of 24 NtMADS-box genes were confirmed by quantitative real-time PCR. Importantly, overexpressed NtSOC1/NtMADS133 could promote early flowering and dwarfism in transgenic tobacco plants. Therefore, our findings provide insights on the characterization of NtMADS-box genes to further study their functions in plant development.

scholarly journals Utilizing MIKC-type MADS-box protein SOC1 for yield potential enhancement in maize

2021 ◽  
Author(s):  
Guo-qing Song ◽  
Xue Han ◽  
John T. Ryner ◽  
Addie Thompson ◽  
Kan Wang
Keyword(s):  
Transgenic Plants ◽  
Plant Height ◽  
No Reduction ◽  
Planting Density ◽  
Yield Potential ◽  
Grain Production ◽  
Mads Box ◽  
Young Leaves ◽  
Mads Box Gene ◽  
Transcriptome Comparison

Abstract Key message Overexpression of Zea mays SOC gene promotes flowering, reduces plant height, and leads to no reduction in grain production per plant, suggesting enhanced yield potential, at least, through increasing planting density. Abstract MIKC-type MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) is an integrator conserved in the plant flowering pathway. In this study, the maize SOC1 (ZmSOC1) gene was cloned and overexpressed in transgenic maize Hi-II genotype. The T0 plants were backcrossed with nontransgenic inbred B73 to produce first generation backcross (BC1) seeds. Phenotyping of both transgenic and null segregant (NT) BC1 plants was conducted in three independent experiments. The BC1 transgenic plants showed new attributes such as increased vegetative growth, accelerated flowering time, reduced overall plant height, and increased grain weight. Second generation backcross (BC2) plants were evaluated in the field using two planting densities. Compared to BC2 NT plants, BC2 transgenic plants, were 12–18% shorter, flowered 5 days earlier, and showed no reduction in grain production per plant and an increase in fat, starch, and simple sugars in the grain. Transcriptome comparison in young leaves of 56-day-old BC1 plants revealed that the overexpressed ZmSOC1 resulted in 107 differentially expressed genes. The upregulated transcription factor DNA BINDING WITH ONE FINGER 5.4 (DOF5.4) was among the genes responsible for the reduced plant height. Modulating expression of SOC1 opens a new and effective approach to promote flowering and reduce plant height, which may have potential to enhance crop yield and improve grain quality.

scholarly journals The free proline levels in transgenic sunflower (Helianthus annuus L.) T3 plants with double-stranded proline dehydrogenase gene RNA-suppressor

1970 ◽  
pp. 211-214 ◽  
Author(s):  
A. G. Komisarenko ◽  
S. I. Mykhalska
Keyword(s):  
Transgenic Plants ◽  
Helianthus Annuus ◽  
High Elevation ◽  
Stress Conditions ◽  
Free Proline ◽  
Wild Type ◽  
Helianthus Annuus L ◽  
Osmotic Tolerance ◽  
Dehydrogenase Gene ◽  
Plant Shoots

Aim. The investigation of the T3 transgenic sunflower plants osmotic tolerance there were developed. The levels of free proline in plant shoots and roots were estimated. Methods. Mature sunflower plants (T3 and wild type) were cultured in standard pots. Those genotypes were tested during 12-day artificial drying. The levels of free proline in plant shoots and roots were measured. Results. The proline contents in transgenic plants preferred those parameters of control plants both under normal and stress conditions. The proline levels in shoots and roots increased in all genotypes cultivated under stress conditions. The shoot/root proline ratio of control plants was constant during whole experiment, while in T3 plants this parameter changed due to high elevation in roots. Conclusions. The changes of shoot/root proline ratio of T3 plants were the result of free proline transfer among plant organs. Keywords: Helianthus annuus L., transgenic plants, L-proline, shoot/root proline ratio.

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