scholarly journals The Effect of Endophytic Bacteria Bacillus subtilis and Salicylic Acid on Some Resistance and Quality Traits of Stored Solanum tuberosum L. Tubers Infected with Fusarium Dry Rot

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 738 ◽  
Author(s):  
Oksana Lastochkina ◽  
Liudmila Pusenkova ◽  
Darya Garshina ◽  
Ruslan Yuldashev ◽  
Irina Shpirnaya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Salicylic Acid ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Quality Traits ◽  
Dry Rot ◽  
Long Term Storage ◽  
Fusarium Dry Rot ◽  
Term Storage

The effect of endophytic Bacillus subtilis (strains 10-4, 26D) and their compositions with salicylic acid (SA) on some resistance and quality traits of stored potatoes infected with Fusarium dry rot were studied. The experiments were carried out on hydroponically grown Solanum tuberosum L. tubers that were infected before storage with Fusarium oxysporum and coated with B. subtilis 10-4, 26D with and without exogenous SA, and then stored for six months. It has been shown that 10-4, 26D, 10-4 + SA, and 26D + SA reduced in different levels (up to 30–50%) the incidence of F. oxysporum-caused dry rot (with the highest effect for 10-4 + SA). SA notably enhanced the positive effect of 10-4, while for 26D, such an effect was not observed. All of the tested treatments increased amylase (AMY) and AMY inhibitors activity in infected tubers, while decreased Fusarium-induced protease activity (except in the case of 10-4 + SA, which promoted a slight increase) was revealed. 10-4, 26D, and their compositions with SA decreased (in different degrees) the pathogen-caused lipid peroxidation, proline, and reducing sugars accumulation in potatoes after long-term storage. It was also discovered 10-4 and 26D, regardless of SA presence, decrease pathogen-induced glycoalkaloids α-Solanine and α-Chaconine accumulation and preserved increased levels of starch and total dry matter in infected stored potatoes. The findings indicate endophytic B. subtilis and its compositions with SA is a promising eco-friendly and bio-safe approach to cope with postharvest decays of potato during long-term storage; however, when developing preparations-compositions it should take into account the strain-dependent manner of B. subtilis action together with SA.

scholarly journals Degradation of chlorobenzene and 2-chlorotoluene by immobilized bacteria strains Comamonas testosterone kt5 and Bacillus subtilis dkt

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Ha Danh Duc ◽  
Nguyen Thi Oanh
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Strain ◽  
Immobilized Cells ◽  
Resting Cells ◽  
Long Term Storage ◽  
Degradation Rates ◽  
Contributory Negligence ◽  
Biofilm Bacteria ◽  
Term Storage

Chlorobenzenes and chlorotoluenes have been used to produce a number of industrial products. They are toxic and widely detected in environments due to human contributory negligence. In this article, the mixed culture of a toluenes-degrading bacterial strain, Comamonas testosterone KT5 (a Gram-positive, catalase-positive bacterium) and a chlorobenzenes-degrading bacterial strain, Bacillus subtilis DKT (a Gram-negative soil bacterium) effectively degraded both chemical compounds co-contaminating in liquid media. In addition, the degradations of mixed compounds by biofilm, bacteria immobilized in polyurethane foam (PUF) and alginate were determined. The results showed that the degradation of both compounds by cells in alginate was significantly higher than that by suspended cells. Moreover, cells immobilized in these materials showed lower adverse effects than those of non-immobilized cells for long-term storage. For examples, the degradation rates for chlorobenzine and 2-chlorotoluene by resting cells reduced by 39.5% and 37.3% after storage for 4 months at 4°C, while the degradation rates by immobilized cells decreased by from 16.3% to 19.8% respectively. 

scholarly journals Isolation, Identification and Preservation of Fusarium Spp. Causing Dry Rot of Potato Tubers

2017 ◽  
Vol 76 (1) ◽  
pp. 45-51
Author(s):  
Emil Stefańczyk ◽  
Sylwester Sobkowiak
Keyword(s):  
Crop Rotation ◽  
Potato Tubers ◽  
Fusarium Spp ◽  
Single Spore ◽  
Dry Rot ◽  
Crop Losses ◽  
Genus Fusarium ◽  
Long Term Storage ◽  
Term Storage

Abstract Fungi of the genus Fusarium cause dry rot, a potato disease which develops during long-term storage of tubers. The disease-inducing capabilities differ among Fusarium spp., but may also vary within species universally considered main dry rot agents. Identification of Fusarium spp. present on diseased tubers in a surveyed area can help minimize crop losses and mycotoxin contamination by, for example, applying proper fungicides or planning crop rotation. Here, we present a procedure of obtaining single spore colonies of Fusarium spp. from potato tubers infected by dry rot, their identification using molecular methods and ways of preservation.

scholarly journals Thermoadaptation-Directed Enzyme Evolution in an Error-Prone Thermophile Derived from Geobacillus kaustophilus HTA426

2014 ◽  
Vol 81 (1) ◽  
pp. 149-158 ◽  
Author(s):  
Hirokazu Suzuki ◽  
Jyumpei Kobayashi ◽  
Keisuke Wada ◽  
Megumi Furukawa ◽  
Katsumi Doi
Keyword(s):  
Bacillus Subtilis ◽  
Parent Strain ◽  
Enzyme Evolution ◽  
Denaturation Temperature ◽  
Content Type ◽  
Practical Applications ◽  
Long Term Storage ◽  
Geobacillus Kaustophilus ◽  
Term Storage

ABSTRACTThermostability is an important property of enzymes utilized for practical applications because it allows long-term storage and use as catalysts. In this study, we constructed an error-prone strain of the thermophileGeobacillus kaustophilusHTA426 and investigated thermoadaptation-directed enzyme evolution using the strain. A mutation frequency assay using the antibiotics rifampin and streptomycin revealed thatG. kaustophilushad substantially higher mutability thanEscherichia coliandBacillus subtilis. The predominant mutations inG. kaustophiluswere A · T→G · C and C · G→T · A transitions, implying that the high mutability ofG. kaustophiluswas attributable in part to high-temperature-associated DNA damage during growth. Among the genes that may be involved in DNA repair inG. kaustophilus, deletions of themutSL,mutY,ung, andmfdgenes markedly enhanced mutability. These genes were subsequently deleted to construct an error-prone thermophile that showed much higher (700- to 9,000-fold) mutability than the parent strain. The error-prone strain was auxotrophic for uracil owing to the fact that the strain was deficient in the intrinsicpyrFgene. Although the strain harboringBacillus subtilispyrFwas also essentially auxotrophic, cells became prototrophic after 2 days of culture under uracil starvation, generatingB. subtilisPyrF variants with an enhanced half-denaturation temperature of >10°C. These data suggest that this error-prone strain is a promising host for thermoadaptation-directed evolution to generate thermostable variants from thermolabile enzymes.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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