Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66

Fusarium head blight (FHB) is a destructive disease of wheat (Triticum aestivum L.), which not only significantly reduces grain yield, but also affects end-use quality. Breeding wheat cultivars with high FHB resistance is the most effective way to control the disease. The Chinese wheat cultivar Jingzhou 66 (JZ66) shows moderately high FHB resistance; however, the genetic basis of its resistance is unknown. A doubled haploid (DH) population consisting 209 lines was developed from a cross of JZ66 and Aikang 58 (AK58), a FHB susceptible wheat cultivar, to identify quantitative trait loci (QTL) that contribute to the FHB resistance. Five field experiments were established across two consecutive crop seasons (2018 and 2019) to evaluate the DH lines and parents for FHB response. The parents and DH population were genotyped with the wheat 55K single-nucleotide polymorphism (SNP) assay. Six QTLs associated with FHB resistance in JZ66 were mapped on chromosome 2DS, 3AS, 3AL, 3DL, 4DS, and 5DL, respectively. Four of the QTL (QFhb.hbaas-2DS, QFhb.hbaas-3AL, QFhb.hbaas-4DS, and QFhb.hbaas-5DL) were detected in at least two environments, and the QTL on 3AL and 5DL might be new. The QTL with major effects, QFhb.hbaas-2DS and QFhb.hbaas-4DS, explained up to 36.2% and 17.6% of the phenotypic variance, and were co-localized with the plant semi-dwarfing loci Rht8 and Rht-D1. The dwarfing Rht8 allele significantly increased spike compactness (SC) and FHB susceptibility causing a larger effect on FHB response than Rht-D1 observed in this study. PCR–based SNP markers for QFhb.hbaas-2DS, QFhb.hbaas-3AL, QFhb.hbaas-4DS, and QFhb.hbaas-5DL, were developed to facilitate their use in breeding for FHB resistance by marker-assisted selection.

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A spontaneous segmental deletion from chromosome arm 3DL enhances Fusarium head blight resistance in wheat

Genome ◽

10.1139/gen-2015-0088 ◽

2015 ◽

Vol 58 (11) ◽

pp. 479-488 ◽

Cited By ~ 7

Author(s):

David F. Garvin ◽

Hedera Porter ◽

Zachary J. Blankenheim ◽

Shiaoman Chao ◽

Ruth Dill-Macky

Keyword(s):

Molecular Markers ◽

Fusarium Head Blight ◽

Molecular Mapping ◽

Wheat Cultivar ◽

Fusarium Head Blight Resistance ◽

Disease Spread ◽

Resistance Locus ◽

Head Blight ◽

Chromosome Arm ◽

Fhb Resistance

Much effort has been directed at identifying sources of resistance to Fusarium head blight (FHB) in wheat. We sought to identify molecular markers for what we hypothesized was a new major FHB resistance locus originating from the wheat cultivar ‘Freedom’ and introgressed into the susceptible wheat cultivar ‘USU-Apogee’. An F2:3 mapping population from a cross between Apogee and A30, its BC4 near-isoline exhibiting improved FHB resistance, was evaluated for resistance. The distribution of FHB resistance in the population approximated a 1:3 moderately resistant : moderately susceptible + susceptible ratio. Separate disease evaluations established that A30 accumulated less deoxynivalenol and yielded a greater proportion of sound grain than Apogee. Molecular mapping revealed that the FHB resistance of A30 is associated with molecular markers on chromosome arm 3DL that exhibit a null phenotype in A30 but are present in both Apogee and Freedom, indicating a spontaneous deletion occurred during the development of A30. Aneuploid analysis revealed that the size of the deleted segment is approximately 19% of the arm’s length. Our results suggest that the deleted interval of chromosome arm 3DL in Apogee may harbor FHB susceptibility genes that promote disease spread in infected spikes, and that their elimination increases FHB resistance in a novel manner.

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Screening of an EMS mutagenized population of a wheat cultivar susceptible to Fusarium head blight identifies resistant variants

Plant Disease ◽

10.1094/pdis-03-21-0670-re ◽

2021 ◽

Author(s):

Bhavit Chhabra ◽

Lovepreet Singh ◽

Sydney Wallace ◽

Adam Schoen ◽

Yanhong Dong ◽

...

Keyword(s):

Fusarium Head Blight ◽

Wheat Cultivar ◽

Genetic Resistance ◽

Field Testing ◽

Head Blight ◽

Point Of Sale ◽

Alternative Approaches ◽

Small Grains ◽

Fhb Resistance ◽

Mutant Lines

Fusarium head blight (FHB) primarily caused by Fusarium graminearum is a key disease of small grains. Diseased spikes show symptoms of premature bleaching shortly after infection and have aborted or shriveled seeds, resulting in reduced yields. The fungus also deteriorates quality and safety of the grain due to production of mycotoxins, especially deoxynivalenol (DON), which can result in grain being docked or rejected at the point of sale. Genetic host resistance to FHB is quantitative and no complete genetic resistance against this devastating disease is available. Alternative approaches to develop new sources of FHB resistance are needed. In this study, we performed extensive forward genetic screening of the M4 generation of an EMS induced mutagenized population of cultivar Jagger to isolate variants with FHB resistance. In field testing, 74 mutant lines were found to have resistance against FHB spread and 30 lines out of these also had low DON content. Subsequent testing over two years in controlled greenhouse conditions revealed ten M6 lines showing significantly lower FHB spread. Seven and six lines out of those 10 lines also had reduced DON content and lower FDKs, respectively. Future endeavors will include identification of the mutations that led to resistance in these variants.

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Molecular mapping of a recessive stripe rust resistance gene yrMY37 in Chinese wheat cultivar Mianmai 37

Molecular Breeding ◽

10.1007/s11032-015-0293-6 ◽

2015 ◽

Vol 35 (3) ◽

Cited By ~ 11

Author(s):

Yong Ren ◽

Shengrong Li ◽

Xianchun Xia ◽

Qiang Zhou ◽

Yuanjiang He ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Molecular Mapping ◽

Wheat Cultivar ◽

Rust Resistance Gene ◽

Stripe Rust Resistance ◽

Stripe Rust Resistance Gene ◽

Chinese Wheat Cultivar ◽

Chinese Wheat

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Genetics of Fusarium head blight resistance in three wheat genotypes

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.3.11 ◽

2019 ◽

Vol 79 (3) ◽

Author(s):

Satish . Kumar ◽

M. S. Saharan ◽

Vipin . Panwar ◽

Ravish . Chatrath ◽

G. P. Singh

Keyword(s):

Fusarium Head Blight ◽

Animal Health ◽

Fusarium Head Blight Resistance ◽

Plant Diseases ◽

Complex Nature ◽

Head Blight ◽

Sources Of Resistance ◽

Chinese Wheat Cultivar ◽

Chinese Wheat ◽

Sumai 3

Fusarium head blight (FHB) is global concern as recent outbreaks reported in Canada, Europe, Asia, Australia and South America. The disease has emerged as one of the most important plant diseases worldwide in 21st century. One of the major threats posed by FHB fungus is the mycotoxin production which is harmful to human and animal health. Development of disease resistant cultivars is the only effective method for managing the disease. Control of these pathogen / Fusarium spp. is also challenging due to limited sources of known resistance. The famous Chinese wheat cultivar Sumai 3 and Frontana are the main sources of resistance to this disease. For genetic analysis and incorporation of FHB resistance into recently released high yielding wheat cultivars, HD 2967 and DPW 621-50, crosses were made with Sumai 3, Frontana and Aldan. The F2 plants from the crosses HD 2967/Frontana (140), HD 2967/Aldan (150), HD 2967/Sumai 3 (169) and DPW 621-50/Sumai 3 (182) were screened for resistance under controlled conditions. Disease score was recorded to identify resistant, moderately resistant and susceptible plants. The genetic ratios for resistance to FHB indicated a complex nature of resistance in all the three donors.

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Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana

Theoretical and Applied Genetics ◽

10.1007/s00122-004-1620-1 ◽

2004 ◽

Vol 109 (1) ◽

pp. 215-224 ◽

Cited By ~ 154

Author(s):

B. Steiner ◽

M. Lemmens ◽

M. Griesser ◽

U. Scholz ◽

J. Schondelmaier ◽

...

Keyword(s):

Fusarium Head Blight ◽

Spring Wheat ◽

Molecular Mapping ◽

Wheat Cultivar ◽

Head Blight ◽

Spring Wheat Cultivar

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Molecular mapping of novel genes controllingFusariumhead blight resistance and deoxynivalenol accumulation in spring wheat

Genome ◽

10.1139/g03-033 ◽

2003 ◽

Vol 46 (4) ◽

pp. 555-564 ◽

Cited By ~ 184

Author(s):

Daryl J Somers ◽

George Fedak ◽

Marc Savard

Keyword(s):

Triticum Aestivum ◽

Fusarium Head Blight ◽

Molecular Mapping ◽

Field Trials ◽

Disease Spread ◽

Head Blight ◽

Sources Of Resistance ◽

Spike Morphology ◽

Spray Inoculation ◽

Fhb Resistance

Fusarium head blight of wheat is an extremely damaging disease, causing severe losses in seed yield and quality. The objective of the current study was to examine and characterize alternate sources of resistance to Fusarium head blight (FHB). Ninety-one F1-derived doubled haploid lines from the cross Triticum aestivum 'Wuhan-1' × Triticum aestivum 'Maringa' were examined for disease reaction to Fusarium graminearum by single-floret injection in replicated greenhouse trials and by spray inoculation in replicated field trials. Field and greenhouse experiments were also used to collect agronomic and spike morphology characteristics. Seed samples from field plots were used for deoxynivalenol (DON) determination. A total of 328 polymorphic microsatellite loci were used to construct a genetic linkage map in this population and together these data were used to identify QTL controlling FHB resistance, accumulation of DON, and agronomic and spike morphology traits. The analysis identified QTL for different types of FHB resistance in four intervals on chromosomes 2DL, 3BS, and 4B. The QTLs on 4B and 3BS proximal to the centromere are novel and not reported elsewhere. QTL controlling accumulation of DON independent of FHB resistance were located on chromosomes 2DS and 5AS. Lines carrying FHB resistance alleles on 2DL and 3BS showed a 32% decrease in disease spread after single-floret injection. Lines carrying FHB resistance alleles on 3BS and 4B showed a 27% decrease from the mean in field infection. Finally, lines carrying favourable alleles on 3BS and 5AS, showed a 17% reduction in DON accumulation. The results support a polygenic and quantitative mode of inheritance and report novel FHB resistance loci. The data also suggest that resistance to FHB infection and DON accumulation may be controlled, in part, by independent loci and (or) genes.Key words: marker-assisted selection, Fusarium, wheat, microsatellite.

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Molecular Mapping of QTL for Fusarium Head Blight Resistance in a Doubled Haploid Population of Chinese Bread Wheat

Plant Disease ◽

10.1094/pdis-06-20-1186-re ◽

2020 ◽

Author(s):

Zhanwang Zhu ◽

Xiaoting Xu ◽

Luping Fu ◽

Fengju Wang ◽

Yachao Dong ◽

...

Keyword(s):

Fusarium Head Blight ◽

Plant Height ◽

Doubled Haploid ◽

Agronomic Traits ◽

Natural Infection ◽

Snp Array ◽

Head Blight ◽

Dh Population ◽

Anther Extrusion ◽

Fhb Resistance

Fusarium head blight (FHB) is a destructive disease of wheat worldwide, particularly in China. To map genetic loci underlying FHB resistance, a doubled haploid (DH) population consisting of 174 lines was developed from a cross between widely grown Chinese cultivars Yangmai 16 and Zhongmai 895. The DH population and parents were evaluated in field nurseries at Wuhan in 2016–2017 and 2017–2018 with both spray inoculation and natural infection, and at Jingzhou in 2017–2018 with grain-spawn inoculation. The DH lines were genotyped with a wheat 660K SNP array. FHB index, plant height, anther extrusion, and days to anthesis were recorded and used for QTL analysis. Seven QTL for FHB resistance were mapped to chromosome arms 3BL, 4AS, 4BS, 4DS, 5AL, 6AL, and 6BS, in at least two environments. QFhb.caas-4BS and QFhb.caas-4DS co-located with semi-dwarfing alleles Rht-B1b and Rht-D1b, respectively, and were also associated with anther extrusion. The other five QTL were genetically independent of the agronomic traits, indicating their potential value in breeding for FHB resistance. Based on correlations between FHB indices and agronomic traits in this population, we concluded that increasing plant height to some extent would enhance FHB resistance, anther extrusion played a more important role in environments with less severe FHB, and days to anthesis was independent of FHB response when viewed across years. PCR-based markers were developed for the 3BL and 5AL QTL, which were detected in more than three environments. The InDel marker InDel_AX-89588684 for QFhb.caas-5AL was also validated on a wheat panel, confirming its effectiveness for marker-assisted breeding for improvement of FHB resistance.

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Relationship between Fusarium Head Blight, Kernel Damage, Concentration of Fusarium Biomass, and Fusarium Toxins in Grain of Winter Wheat Inoculated with Fusarium culmorum

Toxins ◽

10.3390/toxins11010002 ◽

2018 ◽

Vol 11 (1) ◽

pp. 2 ◽

Cited By ~ 5

Author(s):

Tomasz Góral ◽

Halina Wiśniewska ◽

Piotr Ochodzki ◽

Linda Nielsen ◽

Dorota Walentyn-Góral ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Field Experiments ◽

Fusarium Culmorum ◽

Fusarium Toxins ◽

Head Blight ◽

Different Types ◽

Wide Variability ◽

Fhb Resistance ◽

Wheat Lines

Winter wheat lines were evaluated for their reaction to Fusarium head blight (FHB) after inoculation with Fusarium culmorum in two field experiments. A mixture of two F. culmorum chemotypes was applied (3ADON—deoxynivalenol producing, NIV—nivalenol producing). Different types of resistance were evaluated, including head infection, kernel damage, Fusarium biomass content and trichothecenes B (deoxynivalenol (DON), and nivalenol (NIV)) accumulation in grain. The aim of the study was to find relationships between different types of resistance. Head infection (FHB index) and Fusarium damaged kernels (FDK) were visually scored. Fusarium biomass was analysed using real-time PCR. Trichothecenes B accumulation was analysed using gas chromatography. Wheat lines differ in their reaction to inoculation for all parameters describing FHB resistance. We found a wide variability of FHB indexes, FDK, and Fusarium biomass content. Both toxins were present. DON content was about 60% higher than NIV and variability of this proportion between lines was observed. Significant correlation was found between head infection symptoms and FDK. Head infection was correlated with F. culmorum biomass and NIV concentration in grain. No correlation was found between the FHB index and DON concentration. Similarly, FDK was not correlated with DON content, but it was with NIV content; however, the coefficients were higher than for the FHB index. Fusarium biomass amount was positively correlated with both toxins as well as with the FHB index and FDK. Environmental conditions significantly influenced the DON/NIV ratio in grain. In locations where less F. culmorum biomass was detected, the DON amount was higher than NIV, while in locations where more F. culmorum biomass was observed, NIV prevailed over DON.

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