scholarly journals Fully Automated Cultivation of Adipose-Derived Stem Cells in the StemCellDiscovery—A Robotic Laboratory for Small-Scale, High-Throughput Cell Production Including Deep Learning-Based Confluence Estimation

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 575
Author(s):  
Jelena Ochs ◽  
Ferdinand Biermann ◽  
Tobias Piotrowski ◽  
Frederik Erkens ◽  
Bastian Nießing ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deep Learning ◽  
High Throughput ◽  
High Speed ◽  
New Technologies ◽  
Human Mesenchymal Stem Cells ◽  
Simulation Software ◽  
System Capacity ◽  
Small Scale ◽  
Production Environment

Laboratory automation is a key driver in biotechnology and an enabler for powerful new technologies and applications. In particular, in the field of personalized therapies, automation in research and production is a prerequisite for achieving cost efficiency and broad availability of tailored treatments. For this reason, we present the StemCellDiscovery, a fully automated robotic laboratory for the cultivation of human mesenchymal stem cells (hMSCs) in small scale and in parallel. While the system can handle different kinds of adherent cells, here, we focus on the cultivation of adipose-derived hMSCs. The StemCellDiscovery provides an in-line visual quality control for automated confluence estimation, which is realized by combining high-speed microscopy with deep learning-based image processing. We demonstrate the feasibility of the algorithm to detect hMSCs in culture at different densities and calculate confluences based on the resulting image. Furthermore, we show that the StemCellDiscovery is capable of expanding adipose-derived hMSCs in a fully automated manner using the confluence estimation algorithm. In order to estimate the system capacity under high-throughput conditions, we modeled the production environment in a simulation software. The simulations of the production process indicate that the robotic laboratory is capable of handling more than 95 cell culture plates per day.

scholarly journals High Throughput Transcriptome Profiling of Lithium Stimulated Human Mesenchymal Stem Cells Reveals Priming towards Osteoblastic Lineage

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55769 ◽  
Author(s):  
Neeraj Kumar Satija ◽  
Deepa Sharma ◽  
Farhat Afrin ◽  
Rajendra P. Tripathi ◽  
Gurudutta Gangenahalli
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Human Mesenchymal Stem Cells ◽  
Transcriptome Profiling ◽  
Osteoblastic Lineage

scholarly journals Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Gerrit Erdmann ◽  
Michael Suchanek ◽  
Patrick Horn ◽  
Fabian Graf ◽  
Christian Volz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Human Mesenchymal Stem Cells ◽  
Rnai Screening

An Investigation of a Relational Database Approach to a Multidisciplinary Conceptual Design for the HSCT

1996 ◽  
Author(s):  
Neil S. Hall ◽  
Robert E. Fulton
Keyword(s):  
Data Management ◽  
Design Process ◽  
Relational Database ◽  
High Speed ◽  
Data Exchange ◽  
New Technologies ◽  
Aircraft Design ◽  
Small Scale ◽  
Design Environment ◽  
New Concepts

Abstract New aerospace designs will incorporate new concepts as a result of advances made in the scientific and engineering technologies. These new concepts will afford the aircraft designer with an interesting and somewhat envious dilemma. The aircraft designer will have unprecedented flexibility in design concepts. However, this new flexibility will often be paralleled in ever increasing design complexity. Aircraft such as the High Speed Civil Transport (HSCT) will provide a design environment which will require the efficient use of new technologies in an arena which has historically proven to have stringent performance and cost goals which must be met in order to result in a successful design. The complexity of the HSCT design will dictate a close multidisciplinary effort requiring large amounts of data exchange. Moreover, with the enormous development costs associated with such a design, corporate teaming is essential. It is critical to the success of the HSCT and future aircraft design that a new approach be taken toward the management and exchange of information. A top-down data management design structure should be developed and implemented in the early stages in order to optimize the design process. A small scale multidisciplinary relational database management design has been developed for the HSCT in order to gain a better understanding of how efficient data management can optimize the aircraft design process.

Design and Evaluation of Flexible Gas Fired Forging System

1994 ◽  
Author(s):  
R. K. Clark ◽  
Pantulu V. Avasarala ◽  
Athamaram H. Soni
Keyword(s):  
Simulation Model ◽  
High Speed ◽  
Material Handling ◽  
New Technologies ◽  
Design Procedure ◽  
Simulation Software ◽  
Material Handling Systems ◽  
Automated Material Handling ◽  
Automated Material Handling Systems

Abstract The advances in the furnace and high speed press technologies for forging applications necessitate the installation of automated material handling systems. In order to study the effect of integrating all these three new technologies, a simulation model of a flexible forging system (FFS) concept is developed. In this work a design procedure of the FFS is developed. A FFS is designed and evaluated. A simulation model of the FFS is developed on a computer using AutoMod simulation software. The developed model is simulated for various scenarios and the results are presented in this paper.

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