Solubility Temperature Dependence of Bio-Based Levulinic Acid, Furfural, and Hydroxymethylfurfural in Water, Nonpolar, Polar Aprotic and Protic Solvents

Bio-based levulinic acid (LA), furfural (FF), and hydroxymethylfurfural (HMF) represent key chemical intermediates when biorefining biomass resources, i.e., either cellulose, glucose, hexoses, etc. (HMF/LA), or hemicellulose, xylose, and pentose (FF). Despite their importance, their online in situ detection by process analytical technologies (PATs), solubility, and its temperature dependence are seldom available. Herein, we report their solubility and temperature dependence by examining n-hexane, cyclohexane, benzene, toluene, 1,4-dioxane, diethyl ether, dichloromethane, tetrahydrofuran, ethyl acetate, acetone, dimethylformamide, acetonitrile, dimethyl sulfoxide, formic acid, n-butanol, n-propanol, ethanol, methanol, and water. These solvents were selected as they are the most common nonpolar, polar aprotic, and polar protic solvents. Fourier-transform infrared (FTIR) spectroscopy was applied as a fast, accurate, and sensitive method to the examined solutions or mixtures. The latter also enables operando monitoring of the investigated compounds in pressurized reactors. Selected temperatures investigated were chosen, as they are within typical operating ranges. The calculated thermodynamic data are vital for designing biorefinery process intensification, e.g., reaction yield optimization by selective compound extraction. In addition to extracting, upstream or downstream unit operations that can benefit from the results include dissolution, crystallization, and precipitation.

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Tissue-based in situ detection of the clarithromycin resistance for the personalized Helicobacter pylori eradication therapy

10.26226/morressier.596dfd5ad462b8029238774d ◽

2017 ◽

Author(s):

Éva Kocsmár

Keyword(s):

Helicobacter Pylori ◽

Eradication Therapy ◽

Helicobacter Pylori Eradication ◽

Clarithromycin Resistance ◽

In Situ Detection ◽

Helicobacter Pylori Eradication Therapy

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Temperature Dependence of Double Shockley Stacking Fault Behavior in Nitrogen-Doped 4h-Sic Studied by In-Situ Synchrotron X-Ray Topography

SSRN Electronic Journal ◽

10.2139/ssrn.3542923 ◽

2020 ◽

Author(s):

Fumihiro Fujie ◽

Shunta Harada ◽

Kenji Hanada ◽

Hiromasa Suo ◽

Haruhiko Koizumi ◽

...

Keyword(s):

Temperature Dependence ◽

Stacking Fault ◽

X Ray ◽

Nitrogen Doped ◽

Fault Behavior

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Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

Water Science & Technology ◽

10.2166/wst.2000.0459 ◽

2000 ◽

Vol 41 (4-5) ◽

pp. 301-308 ◽

Cited By ~ 1

Author(s):

N. Noda ◽

H. Ikuta ◽

Y. Ebie ◽

A. Hirata ◽

S. Tsuneda ◽

...

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Fluorescent Antibody ◽

Nitrifying Bacteria ◽

Fluorescent Antibody Technique ◽

Antibody Technique ◽

Antibody Method ◽

In Situ Detection ◽

Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

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Mechanistische Untersuchungen zum reversiblen Gleichgewicht von metallorganischen Tripeldecker- und Sandwichkomplexen des Typs [Bis{(η5-CpR)Co}-μ-{η4:η4-aren}] und [(η5-CpR)Co(η6-aren)] / Mechanistic Studies towards the Reversible Equilibrium between Metal Organic Triple Decker and Sandwich Complexes [Bis{(η5 -CpR)Co}-μ-{η4 :η4-arene}] and [{(η5-CpR)Co(η6-arene)]

Zeitschrift für Naturforschung B ◽

10.1515/znb-1998-1105 ◽

1998 ◽

Vol 53 (11) ◽

pp. 1267-1272 ◽

Cited By ~ 8

Author(s):

Jörg J. Schneider ◽

Dirk Wolf

Keyword(s):

Ligand Exchange ◽

Sandwich Complexes ◽

Valuable Metal ◽

H Nmr ◽

Synthetic Utility ◽

Metal Organic ◽

Arene Ligand ◽

Benzene Toluene

The arene ligand exchange mechanism of slipped arene triple deckers [Bis{(η5-CpR)Co}-μ-{η4:η4-arene}] (R = Me5, 1,2,4 tri-tert butyl, arene = benzene, toluene) 1 was studied by 1H-NMR spectroscopy for different concentrations and solvents. It has been found that triple deckers of type 1 decompose slowly in solution. A unique equilibrium, between these triple deckers and the mixed sandwich complexes [(η6-arene)Co(η5-CpR)] and 14 e [(η5-Cp)Co]solv fragments generated in situ by decomposition o f 1 exists. In addition to this equilibrium arene lability of the thus formed mixed sandwich complex type has been detected by NMR making slipped triple deckers 1 ideal single source compounds for the generation of two [(η5-Cp)Co] fragments in one reaction step. Such fragments are valuable metal ligand components with high synthetic utility in organometallic chemistry.

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Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

Journal of Clinical Medicine ◽

10.3390/jcm10020319 ◽

2021 ◽

Vol 10 (2) ◽

pp. 319

Author(s):

Hee Cheol Yang ◽

Won Jong Rhee

Keyword(s):

Extracellular Vesicles ◽

Liquid Biopsy ◽

Molecular Beacon ◽

Disease Diagnosis ◽

Single Step ◽

Flow Cytometer ◽

Surface Proteins ◽

Biomarker Detection ◽

In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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In Situ Detection of Local Structure Transformation of 2D SnSe Nanosheets through Nanothermomechanical Behavior

physica status solidi (RRL) - Rapid Research Letters ◽

10.1002/pssr.202100121 ◽

2021 ◽

pp. 2100121

Author(s):

Zhibin Liu ◽

Kunqi Xu ◽

Huarong Zeng ◽

Zhifu Liu ◽

Kunyu Zhao ◽

...

Keyword(s):

Local Structure ◽

Structure Transformation ◽

In Situ Detection

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Renewable Biomass Utilization: A Way Forward to Establish Sustainable Chemical and Processing Industries

Clean Technologies ◽

10.3390/cleantechnol3010014 ◽

2021 ◽

Vol 3 (1) ◽

pp. 243-259

Author(s):

Yadhu N. Guragain ◽

Praveen V. Vadlani

Keyword(s):

Fossil Fuels ◽

Process Intensification ◽

Raw Material ◽

Biomass Composition ◽

Biomass Feedstocks ◽

Renewable Biomass ◽

Sustainability Criteria ◽

Biomass Component ◽

Biomass Resources ◽

Multiple Challenges

Lignocellulosic biomass feedstocks are promising alternatives to fossil fuels for meeting raw material needs of processing industries and helping transit from a linear to a circular economy and thereby meet the global sustainability criteria. The sugar platform route in the biochemical conversion process is one of the promising and extensively studied methods, which consists of four major conversion steps: pretreatment, hydrolysis, fermentation, and product purification. Each of these conversion steps has multiple challenges. Among them, the challenges associated with the pretreatment are the most significant for the overall process because this is the most expensive step in the sugar platform route and it significantly affects the efficiency of all subsequent steps on the sustainable valorization of each biomass component. However, the development of a universal pretreatment method to cater to all types of feedstock is nearly impossible due to the substantial variations in compositions and structures of biopolymers among these feedstocks. In this review, we have discussed some promising pretreatment methods, their processing and chemicals requirements, and the effect of biomass composition on deconstruction efficiencies. In addition, the global biomass resources availability and process intensification ideas for the lignocellulosic-based chemical industry have been discussed from a circularity and sustainability standpoint.

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